Genotypic and phenotypic analysis of S. mutans isolated from dental biofilms formed in vivo under high cariogenic conditions
The oral cavity harbors several Streptococcus mutans genotypes, which could present distinct virulence properties. However, little is known about the diversity and virulence traits of S. mutans genotypes isolated in vivo under controlled conditions of high cariogenic challenge. This study evaluated the genotypic diversity of S. mutans isolated from dental biofilms formed in vivo under sucrose exposure, as well as their acidogenicity and aciduricity. To form biofilms, subjects rinsed their mouths with distilled water or sucrose solution 8 times/day for 3 days. S. mutans collected from saliva and biofilms were genotyped by arbitrarily-primed PCR. Genotypes identified in the biofilms were evaluated regarding their ability to lower the suspension pH through glycolysis and their acid susceptibility and F-ATPase activity. Most subjects harbored only one genotype in saliva, which was detected in almost all biofilm samples at high proportions. Genotypes isolated only in the presence of sucrose had higher acidogenicity than those isolated only in the presence of water. Genotypes from biofilms formed with sucrose were more aciduric after 30 and 60 min of incubation at pH 2.8 and 5.0, respectively. The present results suggest that biofilms formed under high cariogenic conditions may harbor more aciduric and acidogenic S. mutans genotypes.
Main Authors: | Arthur,Rodrigo Alex, Cury,Altair Antoninha Del Bel, Graner,Renata Oliveira Mattos, Rosalen,Pedro Luiz, Vale,Gláuber Campos, Leme,Adriana Franco Paes, Cury,Jaime Aparecido, Tabchoury,Cínthia Pereira Machado |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Fundação Odontológica de Ribeirão Preto
2011
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-64402011000400001 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Genotypic diversity of S. mutans in dental biofilm formed in situ under sugar stress exposure
by: Arthur,Rodrigo Alex, et al.
Published: (2007) -
Recolonization of Mutans Streptococci after Application of Chlorhexidine Gel
by: Vale,Glauber Campos, et al.
Published: (2014) -
Evaluation of genotypic diversity of Streptococcus mutans using distinct arbitrary primers
by: Tabchoury,Cínthia Pereira Machado, et al.
Published: (2008) -
Composition of dental plaque formed in the presence of sucrose and after its interruption
by: Cury,Jaime Aparecido, et al.
Published: (2003) -
The cariogenic dental biofilm: good, bad or just something to control?
by: Wolff,Mark Steven, et al.
Published: (2009)