Three new amides from Streptomyces sp. H7372
Three new amides, methyl phenatate A (1), actiphenamide (2) and actiphenol 1-β-D-glucopyranoside (3), along with thirteen known compounds, were isolated from the organic extract of a fermentation culture of Streptomyces sp. H7372. The structures were elucidated by spectroscopic methods including 1D- and 2D-NMR techniques, and MS analyses. Cycloheximide (6) and cyclo(ΔAla-L-Val) (8) gave a clear zone of inhibition of Ras-Raf-1 interaction in the yeast two-hybrid assay which showed high potency with 10 and 25 µm clear ZOIs on SD His- and inactive on SD His+ at 2.5 µg per disk, respectively.
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Digital revista |
| Language: | English |
| Published: |
Sociedade Brasileira de Química
2011
|
| Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532011000200006 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Three new amides, methyl phenatate A (1), actiphenamide (2) and actiphenol 1-β-D-glucopyranoside (3), along with thirteen known compounds, were isolated from the organic extract of a fermentation culture of Streptomyces sp. H7372. The structures were elucidated by spectroscopic methods including 1D- and 2D-NMR techniques, and MS analyses. Cycloheximide (6) and cyclo(ΔAla-L-Val) (8) gave a clear zone of inhibition of Ras-Raf-1 interaction in the yeast two-hybrid assay which showed high potency with 10 and 25 µm clear ZOIs on SD His- and inactive on SD His+ at 2.5 µg per disk, respectively. |
|---|