On-line LC/UV/MS analysis of flavonols in the three apple varieties most widely cultivated in Brazil
This work describes the first detailed investigation into the chemical structures of flavonols present in the three apple varieties most commonly cultivated in Brazil: Gala, Golden and Fuji. The analyses were carried out by LC/UV/MS (high-performance liquid chromatography coupled to diode array UV detection and mass spectrometry), using post-column addition of UV shift reagents, as well as multiple stage mass spectrometry (MSn) with electrospray ionization in the negative ion mode. Rutin, hyperoside, isoquercitrin, quercitrin, quercetin and three other quercetin-3-pentoside derivatives were identified through the characterization of on-line-based LC/UV/MS data. LC/UV/MS analysis also revealed that the three apple cultivars have similar chromatographic profiles.
Main Authors: | , , , |
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Format: | Digital revista |
Language: | English |
Published: |
Sociedade Brasileira de Química
2007
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Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532007000100011 |
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