Effect of low-level laser therapy on the membrane induced by the Masquelet technique at an orthotopic site in rabbits
ABSTRACT Purpose: To evaluate the low-level laser therapy (LLLT) on the membrane induced by the Masquelet technique in rabbits. Methods: Twelve Norfolk rabbits at approximately 3 months of age were used. A 1-cm segmental defect was induced in both radii, which were filled with polymethylmethacrylate cylinder. LLLT was used postoperatively in the bone defect of one of the forelimbs every 48 hours for 15 days. Six rabbits were euthanatized on third and sixth postoperative weeks. Results: In both forelimbs, radiographs showed new bone growth from radius cut ends on the third postoperative week and more advanced stage on the sixth postoperative week. Ultrasound showed induced membrane one week after the surgery. Histologically, there were no significant differences in the semi-quantitative score of inflammation intensity, total number of blood vessels, bone metaplasia, and collagen. The average thicknesses were 2,050.17 and 1,451.96 μm for control membranes and 2,724.26 and 2,081.03 μm for irradiated membranes, respectively, on third and sixth postoperative weeks. Vascular endothelial growth factor A (VEGF-A) and platelet derived growth factor (PDGF) expression were present in the induced membranes of control and irradiated forelimbs, but there was no significant difference. Conclusions: Based on assessment methods, it was not possible to demonstrate the effect of LLLT on the induced membrane.
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Digital revista |
| Language: | English |
| Published: |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2021
|
| Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502021001000202 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
oai:scielo:S0102-86502021001000202 |
|---|---|
| record_format |
ojs |
| spelling |
oai:scielo:S0102-865020210010002022021-11-19Effect of low-level laser therapy on the membrane induced by the Masquelet technique at an orthotopic site in rabbitsCoris,Jeniffer Gabriela FigueroaRahal,Sheila CaneveseFonseca-Alves,Carlos EduardoMamprim,Maria JaquelineInamassu,Letícia RochaSampaio,Alexandre Botelho de AbreuKano,Washington TakashiMesquita,Luciane dos ReisSilva Junior,José Ivaldo de Siqueira Orthopedics Infrared Rays Low-Level Light Therapy Rabbits ABSTRACT Purpose: To evaluate the low-level laser therapy (LLLT) on the membrane induced by the Masquelet technique in rabbits. Methods: Twelve Norfolk rabbits at approximately 3 months of age were used. A 1-cm segmental defect was induced in both radii, which were filled with polymethylmethacrylate cylinder. LLLT was used postoperatively in the bone defect of one of the forelimbs every 48 hours for 15 days. Six rabbits were euthanatized on third and sixth postoperative weeks. Results: In both forelimbs, radiographs showed new bone growth from radius cut ends on the third postoperative week and more advanced stage on the sixth postoperative week. Ultrasound showed induced membrane one week after the surgery. Histologically, there were no significant differences in the semi-quantitative score of inflammation intensity, total number of blood vessels, bone metaplasia, and collagen. The average thicknesses were 2,050.17 and 1,451.96 μm for control membranes and 2,724.26 and 2,081.03 μm for irradiated membranes, respectively, on third and sixth postoperative weeks. Vascular endothelial growth factor A (VEGF-A) and platelet derived growth factor (PDGF) expression were present in the induced membranes of control and irradiated forelimbs, but there was no significant difference. Conclusions: Based on assessment methods, it was not possible to demonstrate the effect of LLLT on the induced membrane.info:eu-repo/semantics/openAccessSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira v.36 n.10 20212021-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502021001000202en10.1590/acb361003 |
| institution |
SCIELO |
| collection |
OJS |
| country |
Brasil |
| countrycode |
BR |
| component |
Revista |
| access |
En linea |
| databasecode |
rev-scielo-br |
| tag |
revista |
| region |
America del Sur |
| libraryname |
SciELO |
| language |
English |
| format |
Digital |
| author |
Coris,Jeniffer Gabriela Figueroa Rahal,Sheila Canevese Fonseca-Alves,Carlos Eduardo Mamprim,Maria Jaqueline Inamassu,Letícia Rocha Sampaio,Alexandre Botelho de Abreu Kano,Washington Takashi Mesquita,Luciane dos Reis Silva Junior,José Ivaldo de Siqueira |
| spellingShingle |
Coris,Jeniffer Gabriela Figueroa Rahal,Sheila Canevese Fonseca-Alves,Carlos Eduardo Mamprim,Maria Jaqueline Inamassu,Letícia Rocha Sampaio,Alexandre Botelho de Abreu Kano,Washington Takashi Mesquita,Luciane dos Reis Silva Junior,José Ivaldo de Siqueira Effect of low-level laser therapy on the membrane induced by the Masquelet technique at an orthotopic site in rabbits |
| author_facet |
Coris,Jeniffer Gabriela Figueroa Rahal,Sheila Canevese Fonseca-Alves,Carlos Eduardo Mamprim,Maria Jaqueline Inamassu,Letícia Rocha Sampaio,Alexandre Botelho de Abreu Kano,Washington Takashi Mesquita,Luciane dos Reis Silva Junior,José Ivaldo de Siqueira |
| author_sort |
Coris,Jeniffer Gabriela Figueroa |
| title |
Effect of low-level laser therapy on the membrane induced by the Masquelet technique at an orthotopic site in rabbits |
| title_short |
Effect of low-level laser therapy on the membrane induced by the Masquelet technique at an orthotopic site in rabbits |
| title_full |
Effect of low-level laser therapy on the membrane induced by the Masquelet technique at an orthotopic site in rabbits |
| title_fullStr |
Effect of low-level laser therapy on the membrane induced by the Masquelet technique at an orthotopic site in rabbits |
| title_full_unstemmed |
Effect of low-level laser therapy on the membrane induced by the Masquelet technique at an orthotopic site in rabbits |
| title_sort |
effect of low-level laser therapy on the membrane induced by the masquelet technique at an orthotopic site in rabbits |
| description |
ABSTRACT Purpose: To evaluate the low-level laser therapy (LLLT) on the membrane induced by the Masquelet technique in rabbits. Methods: Twelve Norfolk rabbits at approximately 3 months of age were used. A 1-cm segmental defect was induced in both radii, which were filled with polymethylmethacrylate cylinder. LLLT was used postoperatively in the bone defect of one of the forelimbs every 48 hours for 15 days. Six rabbits were euthanatized on third and sixth postoperative weeks. Results: In both forelimbs, radiographs showed new bone growth from radius cut ends on the third postoperative week and more advanced stage on the sixth postoperative week. Ultrasound showed induced membrane one week after the surgery. Histologically, there were no significant differences in the semi-quantitative score of inflammation intensity, total number of blood vessels, bone metaplasia, and collagen. The average thicknesses were 2,050.17 and 1,451.96 μm for control membranes and 2,724.26 and 2,081.03 μm for irradiated membranes, respectively, on third and sixth postoperative weeks. Vascular endothelial growth factor A (VEGF-A) and platelet derived growth factor (PDGF) expression were present in the induced membranes of control and irradiated forelimbs, but there was no significant difference. Conclusions: Based on assessment methods, it was not possible to demonstrate the effect of LLLT on the induced membrane. |
| publisher |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
| publishDate |
2021 |
| url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502021001000202 |
| work_keys_str_mv |
AT corisjeniffergabrielafigueroa effectoflowlevellasertherapyonthemembraneinducedbythemasquelettechniqueatanorthotopicsiteinrabbits AT rahalsheilacanevese effectoflowlevellasertherapyonthemembraneinducedbythemasquelettechniqueatanorthotopicsiteinrabbits AT fonsecaalvescarloseduardo effectoflowlevellasertherapyonthemembraneinducedbythemasquelettechniqueatanorthotopicsiteinrabbits AT mamprimmariajaqueline effectoflowlevellasertherapyonthemembraneinducedbythemasquelettechniqueatanorthotopicsiteinrabbits AT inamassuleticiarocha effectoflowlevellasertherapyonthemembraneinducedbythemasquelettechniqueatanorthotopicsiteinrabbits AT sampaioalexandrebotelhodeabreu effectoflowlevellasertherapyonthemembraneinducedbythemasquelettechniqueatanorthotopicsiteinrabbits AT kanowashingtontakashi effectoflowlevellasertherapyonthemembraneinducedbythemasquelettechniqueatanorthotopicsiteinrabbits AT mesquitalucianedosreis effectoflowlevellasertherapyonthemembraneinducedbythemasquelettechniqueatanorthotopicsiteinrabbits AT silvajuniorjoseivaldodesiqueira effectoflowlevellasertherapyonthemembraneinducedbythemasquelettechniqueatanorthotopicsiteinrabbits |
| _version_ |
1756401071121498112 |