Oxacillin magnetically targeted for the treatment of Methicillin-Resistant S. aureus infection in rats
Abstract Purpose: To evaluate the effect of oxacillin bonded to magnetic nanoparticles in local infection model in rat. Methods: Twelve Wistar rats weighing 290±18g were randomly divided into four groups (n=6, each) and all rats had a magnet ring sutured on their right thighs. In the biodistribution group rats 0.1mL of 99mTc-magnetite (0.66 MBq) was injected i.v and after 30 minutes, biodistribution of 99mTc-magnetite was evaluated in right and left thighs. The other groups were inoculated with MRSA in each thigh muscles. Group 1 rats were injected i.v. with magnetite, group 2 with Magnetite + Oxacillin, group 3 with saline twice a day. After 24 hours samples of muscle secretion were harvested for microbiological analysis; muscle, lungs and kidneys for histology. Results: 99mTc-magnetite uptake was three-fold higher in right thigh muscles (with external magnet) than in the left. In magnetite and oxacillin-magnetite groups, bacterial/CFU was significantly lower in thigh muscles than in saline-controls. The inflammatory reaction in muscles and lungs was significantly lower in oxacillin-magnetite group-rats than in other groups (p<0.001) . Conclusion: This study confirms the potential antimicrobial activity of magnetic nanoparticles for Methicillin-Resistant S. aureus strains, which in addition to concentrate the antibiotic at the infection site, positively influenced the treatment.
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2017
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oai:scielo:S0102-865020170001000462017-02-14Oxacillin magnetically targeted for the treatment of Methicillin-Resistant S. aureus infection in ratsCarvalho,Juliana Fernandes deAzevedo,Ítalo Medeiros deRocha,Keyla Borges FerreiraMedeiros,Aldo CunhaCarriço,Artur da Silva Magnetic Field Therapy Magnetite Nanoparticles Methicillin-Resistant Staphylococcus aureus Rats Abstract Purpose: To evaluate the effect of oxacillin bonded to magnetic nanoparticles in local infection model in rat. Methods: Twelve Wistar rats weighing 290±18g were randomly divided into four groups (n=6, each) and all rats had a magnet ring sutured on their right thighs. In the biodistribution group rats 0.1mL of 99mTc-magnetite (0.66 MBq) was injected i.v and after 30 minutes, biodistribution of 99mTc-magnetite was evaluated in right and left thighs. The other groups were inoculated with MRSA in each thigh muscles. Group 1 rats were injected i.v. with magnetite, group 2 with Magnetite + Oxacillin, group 3 with saline twice a day. After 24 hours samples of muscle secretion were harvested for microbiological analysis; muscle, lungs and kidneys for histology. Results: 99mTc-magnetite uptake was three-fold higher in right thigh muscles (with external magnet) than in the left. In magnetite and oxacillin-magnetite groups, bacterial/CFU was significantly lower in thigh muscles than in saline-controls. The inflammatory reaction in muscles and lungs was significantly lower in oxacillin-magnetite group-rats than in other groups (p<0.001) . Conclusion: This study confirms the potential antimicrobial activity of magnetic nanoparticles for Methicillin-Resistant S. aureus strains, which in addition to concentrate the antibiotic at the infection site, positively influenced the treatment.info:eu-repo/semantics/openAccessSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira v.32 n.1 20172017-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502017000100046en10.1590/s0102-865020170106 |
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Carvalho,Juliana Fernandes de Azevedo,Ítalo Medeiros de Rocha,Keyla Borges Ferreira Medeiros,Aldo Cunha Carriço,Artur da Silva |
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Carvalho,Juliana Fernandes de Azevedo,Ítalo Medeiros de Rocha,Keyla Borges Ferreira Medeiros,Aldo Cunha Carriço,Artur da Silva Oxacillin magnetically targeted for the treatment of Methicillin-Resistant S. aureus infection in rats |
author_facet |
Carvalho,Juliana Fernandes de Azevedo,Ítalo Medeiros de Rocha,Keyla Borges Ferreira Medeiros,Aldo Cunha Carriço,Artur da Silva |
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Carvalho,Juliana Fernandes de |
title |
Oxacillin magnetically targeted for the treatment of Methicillin-Resistant S. aureus infection in rats |
title_short |
Oxacillin magnetically targeted for the treatment of Methicillin-Resistant S. aureus infection in rats |
title_full |
Oxacillin magnetically targeted for the treatment of Methicillin-Resistant S. aureus infection in rats |
title_fullStr |
Oxacillin magnetically targeted for the treatment of Methicillin-Resistant S. aureus infection in rats |
title_full_unstemmed |
Oxacillin magnetically targeted for the treatment of Methicillin-Resistant S. aureus infection in rats |
title_sort |
oxacillin magnetically targeted for the treatment of methicillin-resistant s. aureus infection in rats |
description |
Abstract Purpose: To evaluate the effect of oxacillin bonded to magnetic nanoparticles in local infection model in rat. Methods: Twelve Wistar rats weighing 290±18g were randomly divided into four groups (n=6, each) and all rats had a magnet ring sutured on their right thighs. In the biodistribution group rats 0.1mL of 99mTc-magnetite (0.66 MBq) was injected i.v and after 30 minutes, biodistribution of 99mTc-magnetite was evaluated in right and left thighs. The other groups were inoculated with MRSA in each thigh muscles. Group 1 rats were injected i.v. with magnetite, group 2 with Magnetite + Oxacillin, group 3 with saline twice a day. After 24 hours samples of muscle secretion were harvested for microbiological analysis; muscle, lungs and kidneys for histology. Results: 99mTc-magnetite uptake was three-fold higher in right thigh muscles (with external magnet) than in the left. In magnetite and oxacillin-magnetite groups, bacterial/CFU was significantly lower in thigh muscles than in saline-controls. The inflammatory reaction in muscles and lungs was significantly lower in oxacillin-magnetite group-rats than in other groups (p<0.001) . Conclusion: This study confirms the potential antimicrobial activity of magnetic nanoparticles for Methicillin-Resistant S. aureus strains, which in addition to concentrate the antibiotic at the infection site, positively influenced the treatment. |
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
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2017 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502017000100046 |
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