Pentoxifylline and prostaglandin E1 action on ischemia and reperfusion of small intestine tissue in rats. An immunohistochemical study
PURPOSE: To investigate the action of pentoxifylline (PTX) and prostaglandin E1 (PGE1) on ischemia and reperfusion of small intestine tissue in rats, using immunohistochemical analysis. METHODS: Thirty-five Wistar rats were distributed as follows: group A (n=10): subjected to intestinal ischemia and reperfusion for 60 min, with no drugs; group B (n=10): PTX given during tissue ischemia and reperfusion; group C (n=10): PGE1 given during tissue ischemia and reperfusion; group D (n=5): sham. A segment of the small intestine was excised from each euthanized animal and subjected to immunohistochemical examination. RESULTS: Mean number of cells expressing anti-FAS ligand in the crypts was highest in Group A (78.9 ± 17.3), followed by groups B (16.7 ± 2.8), C (11.3 ± 1.8), and D (2.5 ± 0.9), with very significant differences between groups (p<0.0001). CONCLUSIONS: The use of pentoxifylline or prostaglandin E1 proved beneficial during tissue reperfusion. The immunohistochemical results demonstrated a decrease in apoptotic cells, while protecting other intestinal epithelium cells against death after reperfusion, allowing these cells to renew the epithelial tissue.
Main Authors: | Brasileiro,José Lacerda, Ramalho,Rondon Tosta, Aydos,Ricardo Dutra, Silva,Iandara Schettert, Takita,Luis Carlos, Marks,Guido, Assis,Peterson Vieira de |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2015
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502015000200115 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Ischemia and reperfusion of rat small intestine using pentoxyfilline and prostaglandin E1
by: Brasileiro,José Lacerda, et al.
Published: (2013) -
The role of ischemic preconditioning and pentoxifylline in intestinal ischemia/reperfusion injury of rats
by: Oliveira,Teresinha Regina Ribeiro de, et al.
Published: (2017) -
Pretreatment with pentoxifylline attenuates lung injury induced by intestinal ischemia/reperfusion in rats
by: Marqui,Carlos Eduardo, et al.
Published: (2011) -
The effects of pentoxifylline into the kidneys of rats in a model of unilateral hindlimb ischemia/reperfusion injury
by: Teruya,Roberto, et al.
Published: (2008) -
Pentoxifylline associated to hypertonic saline solution attenuates inflammatory process and apoptosis after intestinal ischemia/reperfusion in rats
by: Marques,Geraldo Magela Nogueira, et al.
Published: (2014)