Study of superoxide dismutase's expression in the colon produced by azoxymethane and inositol hexaphosfate's paper, in mice
PURPOSE: To investigate the expression of superoxide dismutase (SOD), with use of antioxidant inositol hexaphosfate, in the presence of the carcinogen azoxymethane, in FCA of colon rats. METHODS: Wistar rats (n=48) were distributed in four groups of 12 mice. Divided in control (n=12); with azoxymethane administration AOM (n=12); administration of IP6 (n=12) and with administration of IP6/AOM (n=12). The subcutaneous administration of azoxymethane happened in the week 3 and 4 of the experiment, in dose 20mg/Kg, weekly; and administration of IP6 to 1% in water of drinking for 6 weeks in the group 3 and 4. The identification of the expression SOD-1 was accomplished through the quantification imunohistochemistry by the image processing attended by computer in crypts and focus of aberrant crypts in right colon. RESULTS: The group control presented expression of SOD1, on average 16,0%; group AOM, 26,7%; group IP6, 16,9%; group IP6/AOM, 20,9%. Variance analysis among the groups, was calculated 0,0078. CONCLUSION: The azoxymethane increase expression SOD1, while inositol hexaphosphate decreases in a significant way the expression of SOD1 promoted by the administration of the carcinogen azoxymethane.
Main Authors: | Amaral,Eva Glória Abrão Siufi do, Fagundes,Djalma José, Marks,Guido, Inouye,Celso Massachi |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2006
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502006001000007 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
The role of Fas ligand protein in the oxidative stress induced by azoxymethane on crypt colon of rats
by: Bussuan,Luiz Antonio Maksoud, et al.
Published: (2010) -
Modulation of transforming growth factor beta2 (TGF-beta2) by inositol hexaphosphate in colon carcinogenesis in rats
by: Marks,Guido, et al.
Published: (2006) -
Apoptotic effects of inositol hexaphosphate on biomarker Itpr3 in induced colon rat carcinogenesis
by: Guido,Marks, et al.
Published: (2008) -
Enzyme activity of superoxide dismutase and zincemia in women with preeclampsia
by: Araùjo Brito,José, et al.
Published: (2013) -
Identification of excreted iron superoxide dismutase for the diagnosis of Phtytomonas
by: Marín,Clotilde, et al.
Published: (2006)