Impact of light quality on flavonoid production and growth of Hyptis marrubioides seedlings cultivated in vitro
ABSTRACT Hyptis marrubioides Epling, Lamiaceae, a species from Brazilian Cerrado, has been used against gastrointestinal infections, skin infections, pain, and cramps. Herein, H. marrubioides seedlings were cultured in vitro under different wavelengths (white, blue, green, red, and yellow) with 50 µmol m-2 s-1 irradiance and a 16-h photoperiod. After 20 and 30 days of cultivation, shoot length, leaf number, fresh mass, and dry mass were evaluated. The flavonoid rutin content was determined by the HPLC-DAD method. The shoots were longer in plants cultivated under yellow (16.603 ± 0.790 cm, 1.8-fold), red (15.465 ± 0.461 cm, 1.7-fold), and green (14.677 ± 0.737 cm, 1.6-fold) lights than in control plants exposed to white light (9.203 ± 0.388 cm). The number of leaves increased in plants exposed to red (23.425 ± 1.138, 1.1-fold) and green (22.725 ± 1.814, 1.1-fold) lights, compared to control plants (20.133 ± 0.827). Fresh (0.665 ± 0.048 g, 1.2-fold) and dry (0.066 ± 0.005 g, 1.3-fold) mass of seedlings were the highest in seedlings grown under red light, compared to seedlings grown under white light (0.553 ± 0.048 and 0.028 ± 0.004, respectively). However, rutin production was higher under white (0.308 mg g-1 of dry weight) and blue lights (0.298 mg g-1 of dry weight). Thus, red light induces plant growth and increases leaf number and dry weight in in vitro-cultivated H. marrubioides, whereas blue and white lights promote the greatest rutin accumulation.
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Sociedade Brasileira de Farmacognosia
2017
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oai:scielo:S0102-695X20170004004662017-08-31Impact of light quality on flavonoid production and growth of Hyptis marrubioides seedlings cultivated in vitroPedroso,Rita Cassia N.Branquinho,Núbia Angelica A.Hara,Alessandra C.B.A.M.Costa,Alan CarlosSilva,Fabiano GuimarãesPimenta,Leticia P.Silva,Marcio Luiz A.Cunha,Wilson RobertoPauletti,Patricia M.Januario,Ana Helena Flavonoid Hyptis marrubioides In vitro culture Light quality ABSTRACT Hyptis marrubioides Epling, Lamiaceae, a species from Brazilian Cerrado, has been used against gastrointestinal infections, skin infections, pain, and cramps. Herein, H. marrubioides seedlings were cultured in vitro under different wavelengths (white, blue, green, red, and yellow) with 50 µmol m-2 s-1 irradiance and a 16-h photoperiod. After 20 and 30 days of cultivation, shoot length, leaf number, fresh mass, and dry mass were evaluated. The flavonoid rutin content was determined by the HPLC-DAD method. The shoots were longer in plants cultivated under yellow (16.603 ± 0.790 cm, 1.8-fold), red (15.465 ± 0.461 cm, 1.7-fold), and green (14.677 ± 0.737 cm, 1.6-fold) lights than in control plants exposed to white light (9.203 ± 0.388 cm). The number of leaves increased in plants exposed to red (23.425 ± 1.138, 1.1-fold) and green (22.725 ± 1.814, 1.1-fold) lights, compared to control plants (20.133 ± 0.827). Fresh (0.665 ± 0.048 g, 1.2-fold) and dry (0.066 ± 0.005 g, 1.3-fold) mass of seedlings were the highest in seedlings grown under red light, compared to seedlings grown under white light (0.553 ± 0.048 and 0.028 ± 0.004, respectively). However, rutin production was higher under white (0.308 mg g-1 of dry weight) and blue lights (0.298 mg g-1 of dry weight). Thus, red light induces plant growth and increases leaf number and dry weight in in vitro-cultivated H. marrubioides, whereas blue and white lights promote the greatest rutin accumulation.info:eu-repo/semantics/openAccessSociedade Brasileira de FarmacognosiaRevista Brasileira de Farmacognosia v.27 n.4 20172017-08-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2017000400466en10.1016/j.bjp.2016.12.004 |
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Pedroso,Rita Cassia N. Branquinho,Núbia Angelica A. Hara,Alessandra C.B.A.M. Costa,Alan Carlos Silva,Fabiano Guimarães Pimenta,Leticia P. Silva,Marcio Luiz A. Cunha,Wilson Roberto Pauletti,Patricia M. Januario,Ana Helena |
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Pedroso,Rita Cassia N. Branquinho,Núbia Angelica A. Hara,Alessandra C.B.A.M. Costa,Alan Carlos Silva,Fabiano Guimarães Pimenta,Leticia P. Silva,Marcio Luiz A. Cunha,Wilson Roberto Pauletti,Patricia M. Januario,Ana Helena Impact of light quality on flavonoid production and growth of Hyptis marrubioides seedlings cultivated in vitro |
author_facet |
Pedroso,Rita Cassia N. Branquinho,Núbia Angelica A. Hara,Alessandra C.B.A.M. Costa,Alan Carlos Silva,Fabiano Guimarães Pimenta,Leticia P. Silva,Marcio Luiz A. Cunha,Wilson Roberto Pauletti,Patricia M. Januario,Ana Helena |
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Pedroso,Rita Cassia N. |
title |
Impact of light quality on flavonoid production and growth of Hyptis marrubioides seedlings cultivated in vitro |
title_short |
Impact of light quality on flavonoid production and growth of Hyptis marrubioides seedlings cultivated in vitro |
title_full |
Impact of light quality on flavonoid production and growth of Hyptis marrubioides seedlings cultivated in vitro |
title_fullStr |
Impact of light quality on flavonoid production and growth of Hyptis marrubioides seedlings cultivated in vitro |
title_full_unstemmed |
Impact of light quality on flavonoid production and growth of Hyptis marrubioides seedlings cultivated in vitro |
title_sort |
impact of light quality on flavonoid production and growth of hyptis marrubioides seedlings cultivated in vitro |
description |
ABSTRACT Hyptis marrubioides Epling, Lamiaceae, a species from Brazilian Cerrado, has been used against gastrointestinal infections, skin infections, pain, and cramps. Herein, H. marrubioides seedlings were cultured in vitro under different wavelengths (white, blue, green, red, and yellow) with 50 µmol m-2 s-1 irradiance and a 16-h photoperiod. After 20 and 30 days of cultivation, shoot length, leaf number, fresh mass, and dry mass were evaluated. The flavonoid rutin content was determined by the HPLC-DAD method. The shoots were longer in plants cultivated under yellow (16.603 ± 0.790 cm, 1.8-fold), red (15.465 ± 0.461 cm, 1.7-fold), and green (14.677 ± 0.737 cm, 1.6-fold) lights than in control plants exposed to white light (9.203 ± 0.388 cm). The number of leaves increased in plants exposed to red (23.425 ± 1.138, 1.1-fold) and green (22.725 ± 1.814, 1.1-fold) lights, compared to control plants (20.133 ± 0.827). Fresh (0.665 ± 0.048 g, 1.2-fold) and dry (0.066 ± 0.005 g, 1.3-fold) mass of seedlings were the highest in seedlings grown under red light, compared to seedlings grown under white light (0.553 ± 0.048 and 0.028 ± 0.004, respectively). However, rutin production was higher under white (0.308 mg g-1 of dry weight) and blue lights (0.298 mg g-1 of dry weight). Thus, red light induces plant growth and increases leaf number and dry weight in in vitro-cultivated H. marrubioides, whereas blue and white lights promote the greatest rutin accumulation. |
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Sociedade Brasileira de Farmacognosia |
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2017 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2017000400466 |
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