Aloe vera na criopreservação do sêmen de tambaqui (Colossoma macropomum)

This study aimed to evaluate the extract of Aloe vera (AV) associated or not with 10% Dimethylsulfoxide (DMSO) in cryopreservation of tambaqui semen. For the formation of the pools (n= 14), 30 males were hormonally induced twice. Each pool had the objective motility, curvilinear velocity, straight-line velocity, average path velocity and morphology analyzed before and after cryopreservation of semen. The means for cryopreservation were constituted of Powder Coconut Water-104 diluent added DMSO and/or AV (5 or 10%). After cryopreservation, motility, velocities and morphology were reduced significantly when compared to fresh semen. For sperm motility the best treatment was that using only DMSO (20,86±8,31) and DMSO + 5% AV (15.71±9.77). For the velocities, the worse treatment was DMSO+10% AV. Treatment with only the addition of DMSO had a significantly higher effect than others on percentage of morphologically normal sperm. The mean correlation found was between motilityand the rate of morphologically normal sperm (r = 0.687). In conclusion, the addition of AV does not provide greater protection for spermatozoa during cryopreservation.

Bibliographic Details

Main Authors:	Melo-Maciel,M.A.P., Leite-Castro,L.V., Leite,J.S., Oliveira,M.S., Almeida-Monteiro,P.S., Nunes,J.F., Salmito-Vanderley,C.S.B.
Format:	Digital revista
Language:	Portuguese
Published:	Universidade Federal de Minas Gerais, Escola de Veterinária 2015
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000300945
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