Hepatitis B virus DNA stability in plasma samples under short-term storage at 42°C
We evaluated the stability of hepatitis B virus (HBV) DNA in plasma samples stored at 42°C for external quality assessment (EQA) panels of viral load. To assess the stability of plasma samples containing different concentrations of HBV DNA, serial dilutions of HBV-infected samples with a viral load of 6.40 log(10) IU/mL were made to yield viral loads of 5, 4, and 3 log(10) IU/mL. These were incubated at 42°C for up to 7 days and then frozen at -70°C. Viral load testing for HBV DNA was performed for all samples using COBAS¯ AmpliPrep/COBAS¯ TaqMan¯ HBV Test (v.2.0, Roche, Switzerland). Results were compared with fresh frozen plasma samples as a benchmark to establish acceptable measurements on the days following sample collection. Although the results of this study demonstrated a decrease in HBV DNA viral load ranging from 0.005 to 0.30 log(10) IU/mL after storage at 42°C for up to 7 days, these values did not exceed 0.5 log(10), which is the estimated intra-assay variation for molecular tests. Thus, the insignificant decrease in viral load suggests that shipment of HBV in plasma samples at temperatures of up to 42°C is permissible if they are frozen within 7 days.
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Associação Brasileira de Divulgação Científica
2015
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oai:scielo:S0100-879X20150006005532019-03-19Hepatitis B virus DNA stability in plasma samples under short-term storage at 42°Cde Almeida,R.W.Espírito-Santo,M.P.Sousa,P.S.F.de Almeida,A.J.Lampe,E.Lewis-Ximenez,L.L. HBV Stability studies Real-time PCR We evaluated the stability of hepatitis B virus (HBV) DNA in plasma samples stored at 42°C for external quality assessment (EQA) panels of viral load. To assess the stability of plasma samples containing different concentrations of HBV DNA, serial dilutions of HBV-infected samples with a viral load of 6.40 log(10) IU/mL were made to yield viral loads of 5, 4, and 3 log(10) IU/mL. These were incubated at 42°C for up to 7 days and then frozen at -70°C. Viral load testing for HBV DNA was performed for all samples using COBAS¯ AmpliPrep/COBAS¯ TaqMan¯ HBV Test (v.2.0, Roche, Switzerland). Results were compared with fresh frozen plasma samples as a benchmark to establish acceptable measurements on the days following sample collection. Although the results of this study demonstrated a decrease in HBV DNA viral load ranging from 0.005 to 0.30 log(10) IU/mL after storage at 42°C for up to 7 days, these values did not exceed 0.5 log(10), which is the estimated intra-assay variation for molecular tests. Thus, the insignificant decrease in viral load suggests that shipment of HBV in plasma samples at temperatures of up to 42°C is permissible if they are frozen within 7 days.info:eu-repo/semantics/openAccessAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research v.48 n.6 20152015-06-01info:eu-repo/semantics/reporttext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000600553en10.1590/1414-431x20144040 |
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de Almeida,R.W. Espírito-Santo,M.P. Sousa,P.S.F. de Almeida,A.J. Lampe,E. Lewis-Ximenez,L.L. |
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de Almeida,R.W. Espírito-Santo,M.P. Sousa,P.S.F. de Almeida,A.J. Lampe,E. Lewis-Ximenez,L.L. Hepatitis B virus DNA stability in plasma samples under short-term storage at 42°C |
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de Almeida,R.W. Espírito-Santo,M.P. Sousa,P.S.F. de Almeida,A.J. Lampe,E. Lewis-Ximenez,L.L. |
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de Almeida,R.W. |
title |
Hepatitis B virus DNA stability in plasma samples under short-term storage at 42°C |
title_short |
Hepatitis B virus DNA stability in plasma samples under short-term storage at 42°C |
title_full |
Hepatitis B virus DNA stability in plasma samples under short-term storage at 42°C |
title_fullStr |
Hepatitis B virus DNA stability in plasma samples under short-term storage at 42°C |
title_full_unstemmed |
Hepatitis B virus DNA stability in plasma samples under short-term storage at 42°C |
title_sort |
hepatitis b virus dna stability in plasma samples under short-term storage at 42°c |
description |
We evaluated the stability of hepatitis B virus (HBV) DNA in plasma samples stored at 42°C for external quality assessment (EQA) panels of viral load. To assess the stability of plasma samples containing different concentrations of HBV DNA, serial dilutions of HBV-infected samples with a viral load of 6.40 log(10) IU/mL were made to yield viral loads of 5, 4, and 3 log(10) IU/mL. These were incubated at 42°C for up to 7 days and then frozen at -70°C. Viral load testing for HBV DNA was performed for all samples using COBAS¯ AmpliPrep/COBAS¯ TaqMan¯ HBV Test (v.2.0, Roche, Switzerland). Results were compared with fresh frozen plasma samples as a benchmark to establish acceptable measurements on the days following sample collection. Although the results of this study demonstrated a decrease in HBV DNA viral load ranging from 0.005 to 0.30 log(10) IU/mL after storage at 42°C for up to 7 days, these values did not exceed 0.5 log(10), which is the estimated intra-assay variation for molecular tests. Thus, the insignificant decrease in viral load suggests that shipment of HBV in plasma samples at temperatures of up to 42°C is permissible if they are frozen within 7 days. |
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Associação Brasileira de Divulgação Científica |
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2015 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000600553 |
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