In vitro radioautographic studies of the biodistribution of radiopharmaceuticals on blood elements
In the present study we evaluated the binding of the radiopharmaceuticals sodium pertechnetate (Na 99mTcO4), methylenediphosphonic acid (99mTc-MDP) and glucoheptonate acid (99mTc-GHA) to blood elements using centrifugation and radioautographic techniques. Heparinized blood was incubated with the labelled compounds for 0, 1, 2, 3, 4, 6 and 24 h. Plasma (P) and blood cells (BC) were isolated and precipitated with 5% trichloroacetic acid (TCA), and soluble (SF) and insoluble fractions (IF) were separated. Blood samples were prepared (0 and 24 h) and coated with LM-1 radioautographic emulsions and percent radioactivity (%rad) in P and BC was determined. The binding of Na 99mTcO4 (%rad) to P was 61.2% (0 h) and 46.0% (24 h), and radioautography showed 63.7% (0 h) and 43.3% (24 h). The binding to BC was 38.8% (0 h) and 54.0% (24 h), and radioautography showed 36.3% (0 h) and 56.7% (24 h). 99mTc-MDP study presented 91.1% (0 h) to P and 87.2% (24 h), and radioautography showed 67.9% (0 h) and 67.4% (24 h). The binding to BC was 8.9% (0 h) and 12.8% (24 h), and radioautography showed 32.1% (0 h) and 32.6% (24 h). 99mTc-GHA study was 90.1% (0 h) to P and 79.9% (24 h), and radioautography showed 67.2% (0 h) and 60.1% (24 h). The binding to BC was 9.9% (0 h) and 20.1% (24 h), and radioautography showed 32.8% (0 h) and 39.9% (24 h). The comparison of the obtained results suggests that the binding to plasma and blood cells in the two techniques used (radioautography and centrifugation) is qualitatively in accordance
Main Authors: | , , , , , , , , |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Associação Brasileira de Divulgação Científica
1998
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000200014 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
oai:scielo:S0100-879X1998000200014 |
---|---|
record_format |
ojs |
spelling |
oai:scielo:S0100-879X19980002000141998-10-07In vitro radioautographic studies of the biodistribution of radiopharmaceuticals on blood elementsRipoll-Hamer,E.De-Paula,E.F.Freitas,L.C.Pereira,M.J.S.Fonseca,L.M.Gutfilen,B.Carvalho,J.J.Pôrto,L.C.M.S.Bernardo-Filho,M. radioautography radiopharmaceuticals blood elements In the present study we evaluated the binding of the radiopharmaceuticals sodium pertechnetate (Na 99mTcO4), methylenediphosphonic acid (99mTc-MDP) and glucoheptonate acid (99mTc-GHA) to blood elements using centrifugation and radioautographic techniques. Heparinized blood was incubated with the labelled compounds for 0, 1, 2, 3, 4, 6 and 24 h. Plasma (P) and blood cells (BC) were isolated and precipitated with 5% trichloroacetic acid (TCA), and soluble (SF) and insoluble fractions (IF) were separated. Blood samples were prepared (0 and 24 h) and coated with LM-1 radioautographic emulsions and percent radioactivity (%rad) in P and BC was determined. The binding of Na 99mTcO4 (%rad) to P was 61.2% (0 h) and 46.0% (24 h), and radioautography showed 63.7% (0 h) and 43.3% (24 h). The binding to BC was 38.8% (0 h) and 54.0% (24 h), and radioautography showed 36.3% (0 h) and 56.7% (24 h). 99mTc-MDP study presented 91.1% (0 h) to P and 87.2% (24 h), and radioautography showed 67.9% (0 h) and 67.4% (24 h). The binding to BC was 8.9% (0 h) and 12.8% (24 h), and radioautography showed 32.1% (0 h) and 32.6% (24 h). 99mTc-GHA study was 90.1% (0 h) to P and 79.9% (24 h), and radioautography showed 67.2% (0 h) and 60.1% (24 h). The binding to BC was 9.9% (0 h) and 20.1% (24 h), and radioautography showed 32.8% (0 h) and 39.9% (24 h). The comparison of the obtained results suggests that the binding to plasma and blood cells in the two techniques used (radioautography and centrifugation) is qualitatively in accordanceinfo:eu-repo/semantics/openAccessAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research v.31 n.2 19981998-02-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000200014en10.1590/S0100-879X1998000200014 |
institution |
SCIELO |
collection |
OJS |
country |
Brasil |
countrycode |
BR |
component |
Revista |
access |
En linea |
databasecode |
rev-scielo-br |
tag |
revista |
region |
America del Sur |
libraryname |
SciELO |
language |
English |
format |
Digital |
author |
Ripoll-Hamer,E. De-Paula,E.F. Freitas,L.C. Pereira,M.J.S. Fonseca,L.M. Gutfilen,B. Carvalho,J.J. Pôrto,L.C.M.S. Bernardo-Filho,M. |
spellingShingle |
Ripoll-Hamer,E. De-Paula,E.F. Freitas,L.C. Pereira,M.J.S. Fonseca,L.M. Gutfilen,B. Carvalho,J.J. Pôrto,L.C.M.S. Bernardo-Filho,M. In vitro radioautographic studies of the biodistribution of radiopharmaceuticals on blood elements |
author_facet |
Ripoll-Hamer,E. De-Paula,E.F. Freitas,L.C. Pereira,M.J.S. Fonseca,L.M. Gutfilen,B. Carvalho,J.J. Pôrto,L.C.M.S. Bernardo-Filho,M. |
author_sort |
Ripoll-Hamer,E. |
title |
In vitro radioautographic studies of the biodistribution of radiopharmaceuticals on blood elements |
title_short |
In vitro radioautographic studies of the biodistribution of radiopharmaceuticals on blood elements |
title_full |
In vitro radioautographic studies of the biodistribution of radiopharmaceuticals on blood elements |
title_fullStr |
In vitro radioautographic studies of the biodistribution of radiopharmaceuticals on blood elements |
title_full_unstemmed |
In vitro radioautographic studies of the biodistribution of radiopharmaceuticals on blood elements |
title_sort |
in vitro radioautographic studies of the biodistribution of radiopharmaceuticals on blood elements |
description |
In the present study we evaluated the binding of the radiopharmaceuticals sodium pertechnetate (Na 99mTcO4), methylenediphosphonic acid (99mTc-MDP) and glucoheptonate acid (99mTc-GHA) to blood elements using centrifugation and radioautographic techniques. Heparinized blood was incubated with the labelled compounds for 0, 1, 2, 3, 4, 6 and 24 h. Plasma (P) and blood cells (BC) were isolated and precipitated with 5% trichloroacetic acid (TCA), and soluble (SF) and insoluble fractions (IF) were separated. Blood samples were prepared (0 and 24 h) and coated with LM-1 radioautographic emulsions and percent radioactivity (%rad) in P and BC was determined. The binding of Na 99mTcO4 (%rad) to P was 61.2% (0 h) and 46.0% (24 h), and radioautography showed 63.7% (0 h) and 43.3% (24 h). The binding to BC was 38.8% (0 h) and 54.0% (24 h), and radioautography showed 36.3% (0 h) and 56.7% (24 h). 99mTc-MDP study presented 91.1% (0 h) to P and 87.2% (24 h), and radioautography showed 67.9% (0 h) and 67.4% (24 h). The binding to BC was 8.9% (0 h) and 12.8% (24 h), and radioautography showed 32.1% (0 h) and 32.6% (24 h). 99mTc-GHA study was 90.1% (0 h) to P and 79.9% (24 h), and radioautography showed 67.2% (0 h) and 60.1% (24 h). The binding to BC was 9.9% (0 h) and 20.1% (24 h), and radioautography showed 32.8% (0 h) and 39.9% (24 h). The comparison of the obtained results suggests that the binding to plasma and blood cells in the two techniques used (radioautography and centrifugation) is qualitatively in accordance |
publisher |
Associação Brasileira de Divulgação Científica |
publishDate |
1998 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000200014 |
work_keys_str_mv |
AT ripollhamere invitroradioautographicstudiesofthebiodistributionofradiopharmaceuticalsonbloodelements AT depaulaef invitroradioautographicstudiesofthebiodistributionofradiopharmaceuticalsonbloodelements AT freitaslc invitroradioautographicstudiesofthebiodistributionofradiopharmaceuticalsonbloodelements AT pereiramjs invitroradioautographicstudiesofthebiodistributionofradiopharmaceuticalsonbloodelements AT fonsecalm invitroradioautographicstudiesofthebiodistributionofradiopharmaceuticalsonbloodelements AT gutfilenb invitroradioautographicstudiesofthebiodistributionofradiopharmaceuticalsonbloodelements AT carvalhojj invitroradioautographicstudiesofthebiodistributionofradiopharmaceuticalsonbloodelements AT portolcms invitroradioautographicstudiesofthebiodistributionofradiopharmaceuticalsonbloodelements AT bernardofilhom invitroradioautographicstudiesofthebiodistributionofradiopharmaceuticalsonbloodelements |
_version_ |
1756390904563761152 |