In vitro radioautographic studies of the biodistribution of radiopharmaceuticals on blood elements

In the present study we evaluated the binding of the radiopharmaceuticals sodium pertechnetate (Na 99mTcO4), methylenediphosphonic acid (99mTc-MDP) and glucoheptonate acid (99mTc-GHA) to blood elements using centrifugation and radioautographic techniques. Heparinized blood was incubated with the labelled compounds for 0, 1, 2, 3, 4, 6 and 24 h. Plasma (P) and blood cells (BC) were isolated and precipitated with 5% trichloroacetic acid (TCA), and soluble (SF) and insoluble fractions (IF) were separated. Blood samples were prepared (0 and 24 h) and coated with LM-1 radioautographic emulsions and percent radioactivity (%rad) in P and BC was determined. The binding of Na 99mTcO4 (%rad) to P was 61.2% (0 h) and 46.0% (24 h), and radioautography showed 63.7% (0 h) and 43.3% (24 h). The binding to BC was 38.8% (0 h) and 54.0% (24 h), and radioautography showed 36.3% (0 h) and 56.7% (24 h). 99mTc-MDP study presented 91.1% (0 h) to P and 87.2% (24 h), and radioautography showed 67.9% (0 h) and 67.4% (24 h). The binding to BC was 8.9% (0 h) and 12.8% (24 h), and radioautography showed 32.1% (0 h) and 32.6% (24 h). 99mTc-GHA study was 90.1% (0 h) to P and 79.9% (24 h), and radioautography showed 67.2% (0 h) and 60.1% (24 h). The binding to BC was 9.9% (0 h) and 20.1% (24 h), and radioautography showed 32.8% (0 h) and 39.9% (24 h). The comparison of the obtained results suggests that the binding to plasma and blood cells in the two techniques used (radioautography and centrifugation) is qualitatively in accordance

Saved in:
Bibliographic Details
Main Authors: Ripoll-Hamer,E., De-Paula,E.F., Freitas,L.C., Pereira,M.J.S., Fonseca,L.M., Gutfilen,B., Carvalho,J.J., Pôrto,L.C.M.S., Bernardo-Filho,M.
Format: Digital revista
Language:English
Published: Associação Brasileira de Divulgação Científica 1998
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000200014
Tags: Add Tag
No Tags, Be the first to tag this record!
id oai:scielo:S0100-879X1998000200014
record_format ojs
spelling oai:scielo:S0100-879X19980002000141998-10-07In vitro radioautographic studies of the biodistribution of radiopharmaceuticals on blood elementsRipoll-Hamer,E.De-Paula,E.F.Freitas,L.C.Pereira,M.J.S.Fonseca,L.M.Gutfilen,B.Carvalho,J.J.Pôrto,L.C.M.S.Bernardo-Filho,M. radioautography radiopharmaceuticals blood elements In the present study we evaluated the binding of the radiopharmaceuticals sodium pertechnetate (Na 99mTcO4), methylenediphosphonic acid (99mTc-MDP) and glucoheptonate acid (99mTc-GHA) to blood elements using centrifugation and radioautographic techniques. Heparinized blood was incubated with the labelled compounds for 0, 1, 2, 3, 4, 6 and 24 h. Plasma (P) and blood cells (BC) were isolated and precipitated with 5% trichloroacetic acid (TCA), and soluble (SF) and insoluble fractions (IF) were separated. Blood samples were prepared (0 and 24 h) and coated with LM-1 radioautographic emulsions and percent radioactivity (%rad) in P and BC was determined. The binding of Na 99mTcO4 (%rad) to P was 61.2% (0 h) and 46.0% (24 h), and radioautography showed 63.7% (0 h) and 43.3% (24 h). The binding to BC was 38.8% (0 h) and 54.0% (24 h), and radioautography showed 36.3% (0 h) and 56.7% (24 h). 99mTc-MDP study presented 91.1% (0 h) to P and 87.2% (24 h), and radioautography showed 67.9% (0 h) and 67.4% (24 h). The binding to BC was 8.9% (0 h) and 12.8% (24 h), and radioautography showed 32.1% (0 h) and 32.6% (24 h). 99mTc-GHA study was 90.1% (0 h) to P and 79.9% (24 h), and radioautography showed 67.2% (0 h) and 60.1% (24 h). The binding to BC was 9.9% (0 h) and 20.1% (24 h), and radioautography showed 32.8% (0 h) and 39.9% (24 h). The comparison of the obtained results suggests that the binding to plasma and blood cells in the two techniques used (radioautography and centrifugation) is qualitatively in accordanceinfo:eu-repo/semantics/openAccessAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research v.31 n.2 19981998-02-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000200014en10.1590/S0100-879X1998000200014
institution SCIELO
collection OJS
country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Ripoll-Hamer,E.
De-Paula,E.F.
Freitas,L.C.
Pereira,M.J.S.
Fonseca,L.M.
Gutfilen,B.
Carvalho,J.J.
Pôrto,L.C.M.S.
Bernardo-Filho,M.
spellingShingle Ripoll-Hamer,E.
De-Paula,E.F.
Freitas,L.C.
Pereira,M.J.S.
Fonseca,L.M.
Gutfilen,B.
Carvalho,J.J.
Pôrto,L.C.M.S.
Bernardo-Filho,M.
In vitro radioautographic studies of the biodistribution of radiopharmaceuticals on blood elements
author_facet Ripoll-Hamer,E.
De-Paula,E.F.
Freitas,L.C.
Pereira,M.J.S.
Fonseca,L.M.
Gutfilen,B.
Carvalho,J.J.
Pôrto,L.C.M.S.
Bernardo-Filho,M.
author_sort Ripoll-Hamer,E.
title In vitro radioautographic studies of the biodistribution of radiopharmaceuticals on blood elements
title_short In vitro radioautographic studies of the biodistribution of radiopharmaceuticals on blood elements
title_full In vitro radioautographic studies of the biodistribution of radiopharmaceuticals on blood elements
title_fullStr In vitro radioautographic studies of the biodistribution of radiopharmaceuticals on blood elements
title_full_unstemmed In vitro radioautographic studies of the biodistribution of radiopharmaceuticals on blood elements
title_sort in vitro radioautographic studies of the biodistribution of radiopharmaceuticals on blood elements
description In the present study we evaluated the binding of the radiopharmaceuticals sodium pertechnetate (Na 99mTcO4), methylenediphosphonic acid (99mTc-MDP) and glucoheptonate acid (99mTc-GHA) to blood elements using centrifugation and radioautographic techniques. Heparinized blood was incubated with the labelled compounds for 0, 1, 2, 3, 4, 6 and 24 h. Plasma (P) and blood cells (BC) were isolated and precipitated with 5% trichloroacetic acid (TCA), and soluble (SF) and insoluble fractions (IF) were separated. Blood samples were prepared (0 and 24 h) and coated with LM-1 radioautographic emulsions and percent radioactivity (%rad) in P and BC was determined. The binding of Na 99mTcO4 (%rad) to P was 61.2% (0 h) and 46.0% (24 h), and radioautography showed 63.7% (0 h) and 43.3% (24 h). The binding to BC was 38.8% (0 h) and 54.0% (24 h), and radioautography showed 36.3% (0 h) and 56.7% (24 h). 99mTc-MDP study presented 91.1% (0 h) to P and 87.2% (24 h), and radioautography showed 67.9% (0 h) and 67.4% (24 h). The binding to BC was 8.9% (0 h) and 12.8% (24 h), and radioautography showed 32.1% (0 h) and 32.6% (24 h). 99mTc-GHA study was 90.1% (0 h) to P and 79.9% (24 h), and radioautography showed 67.2% (0 h) and 60.1% (24 h). The binding to BC was 9.9% (0 h) and 20.1% (24 h), and radioautography showed 32.8% (0 h) and 39.9% (24 h). The comparison of the obtained results suggests that the binding to plasma and blood cells in the two techniques used (radioautography and centrifugation) is qualitatively in accordance
publisher Associação Brasileira de Divulgação Científica
publishDate 1998
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000200014
work_keys_str_mv AT ripollhamere invitroradioautographicstudiesofthebiodistributionofradiopharmaceuticalsonbloodelements
AT depaulaef invitroradioautographicstudiesofthebiodistributionofradiopharmaceuticalsonbloodelements
AT freitaslc invitroradioautographicstudiesofthebiodistributionofradiopharmaceuticalsonbloodelements
AT pereiramjs invitroradioautographicstudiesofthebiodistributionofradiopharmaceuticalsonbloodelements
AT fonsecalm invitroradioautographicstudiesofthebiodistributionofradiopharmaceuticalsonbloodelements
AT gutfilenb invitroradioautographicstudiesofthebiodistributionofradiopharmaceuticalsonbloodelements
AT carvalhojj invitroradioautographicstudiesofthebiodistributionofradiopharmaceuticalsonbloodelements
AT portolcms invitroradioautographicstudiesofthebiodistributionofradiopharmaceuticalsonbloodelements
AT bernardofilhom invitroradioautographicstudiesofthebiodistributionofradiopharmaceuticalsonbloodelements
_version_ 1756390904563761152