Reaction of sweet orange cultivars expressing the attacin A gene to 'Candidatus Liberibacter asiaticus' infection

The objective of this work was to evaluate the reaction of four sweet orange cultivars expressing the attacin A gene to 'Candidatus Liberibacter asiaticus' (Las) infection, a bacterium associated to huanglongbing (HLB) disease. Transgenic sweet orange plants of Hamlin, Natal, Pêra, and Valência cultivars, as well as nontransgenic controls received inocula by grafting budwood sections of HLB-infected branches. Disease progression was evaluated through observations of leaf symptoms and by polymerase chain reaction (PCR) analysis, eight months after inoculation. A completely randomized design was used, with four experiments (one for each cultivar) performed simultaneously. Bacteria title was estimated by quantitative PCR (qPCR). HLB symptoms and Las titers were present in nontransgenic and transgenic plants expressing the attacin A gene of the four sweet orange cultivars, eight months after bacteria inoculation. Five transgenic lines (transformation events) of 'Pêra' sweet orange expressing the attacin A gene have significantly lower Las titers in comparison with nontransgenic plants of this cultivar.

Bibliographic Details

Main Authors:	Felipe,Rafaella Teles Arantes, Mourão Filho,Francisco de Assis Alves, Lopes,Silvio Aparecido, Mendes,Beatriz Madalena Januzzi, Behling,Maurel, Pereira Junior,Ernani Vicente
Format:	Digital revista
Language:	English
Published:	Embrapa Secretaria de Pesquisa e Desenvolvimento 2013
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013001100004
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