Tissue culture parameters in sweet orange cultivars

Tissue culture parameters in sweet orange cultivars

The objective of this work was to establish tissue culture parameters for gene transfer in sweet orange cultivars. Epicotyl explants with different ages were cultured with 6-benzylaminopurine (BAP), kanamycin and hygromycin. Shoots were cultured with alpha-naphthaleneacetic acid (NAA) alone or in combination with indole-3-butyric acid (IBA). The requirement of BAP for shoot development was genotype-specific. Epicotyl explants from 35-day-old seedlings produced significantly more shoots per explant in 'Pêra'. Kanamycin inhibited shoot regeneration for the most cultivars. The percentage of shoots that produced roots in 'Pêra' was significantly higher in medium with NAA and IBA than with NAA alone.

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Bibliographic Details
Main Authors: Mendes,Amanda Ferreira da Silva, Cidade,Luciana Cardoso, Manzoli,Gabrielle Novais, Otoni,Wagner Campos, Soares Filho,Walter dos Santos, Costa,Marcio Gilberto Cardoso
Format: Digital revista
Language:English
Published: Embrapa Secretaria de Pesquisa e Desenvolvimento 2008
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000800020
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000800020

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