Trypanosoma cruzi XRNA granules colocalise with distinct mRNP granules at the nuclear periphery
BACKGROUND Eukaryotic ribonucleoprotein (RNP) granules are important for the regulation of RNA fate. RNP granules exist in trypanosomatids; however, their roles in controlling gene expression are still not understood. XRNA is a component of granules in Trypanosoma brucei but has not been investigated in Trypanosoma cruzi. OBJECTIVES This study aimed to investigate the TcXRNA dynamic assembly and its interaction with RNP components under conditions that affect the mRNA availability. METHODS We used in vitro metacyclogenesis of T. cruzi to observe changes in RNP granules during the differentiation process. TcXRNA expression was analysed by Western blot and immunofluorescence. Colocalisation assays were performed to investigate the interaction of TcXRNA with other RNP components. FINDINGS TcXRNA is constantly present during metacyclogenesis and is localised in cytoplasmic granules. TcXRNA does not colocalise with TcDHH1 and TcCAF1 granules in the cytoplasm. However, TcXRNA granules colocalise with mRNP granules at the nuclear periphery when mRNA processing is inhibited. MAIN CONCLUSIONS TcXRNA plays a role in mRNA metabolism as a component of mRNP granules whose assembly is dependent on mRNA availability. TcXRNA granules colocalise with distinct RNP granules at the nuclear periphery, suggesting that the perinuclear region is a regulatory compartment in T. cruzi mRNA metabolism.
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Instituto Oswaldo Cruz, Ministério da Saúde
2018
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oai:scielo:S0074-027620180006003042018-06-14Trypanosoma cruzi XRNA granules colocalise with distinct mRNP granules at the nuclear peripheryCosta,Jimena Ferreira daFerrarini,Mariana GalvãoNardelli,Sheila CristinaGoldenberg,SamuelÁvila,Andréa RodriguesHoletz,Fabíola Barbieri XRNA mRNP granules nuclear periphery metacyclogenesis BACKGROUND Eukaryotic ribonucleoprotein (RNP) granules are important for the regulation of RNA fate. RNP granules exist in trypanosomatids; however, their roles in controlling gene expression are still not understood. XRNA is a component of granules in Trypanosoma brucei but has not been investigated in Trypanosoma cruzi. OBJECTIVES This study aimed to investigate the TcXRNA dynamic assembly and its interaction with RNP components under conditions that affect the mRNA availability. METHODS We used in vitro metacyclogenesis of T. cruzi to observe changes in RNP granules during the differentiation process. TcXRNA expression was analysed by Western blot and immunofluorescence. Colocalisation assays were performed to investigate the interaction of TcXRNA with other RNP components. FINDINGS TcXRNA is constantly present during metacyclogenesis and is localised in cytoplasmic granules. TcXRNA does not colocalise with TcDHH1 and TcCAF1 granules in the cytoplasm. However, TcXRNA granules colocalise with mRNP granules at the nuclear periphery when mRNA processing is inhibited. MAIN CONCLUSIONS TcXRNA plays a role in mRNA metabolism as a component of mRNP granules whose assembly is dependent on mRNA availability. TcXRNA granules colocalise with distinct RNP granules at the nuclear periphery, suggesting that the perinuclear region is a regulatory compartment in T. cruzi mRNA metabolism.info:eu-repo/semantics/openAccessInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz v.113 n.6 20182018-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762018000600304en10.1590/0074-02760170531 |
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Costa,Jimena Ferreira da Ferrarini,Mariana Galvão Nardelli,Sheila Cristina Goldenberg,Samuel Ávila,Andréa Rodrigues Holetz,Fabíola Barbieri |
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Costa,Jimena Ferreira da Ferrarini,Mariana Galvão Nardelli,Sheila Cristina Goldenberg,Samuel Ávila,Andréa Rodrigues Holetz,Fabíola Barbieri Trypanosoma cruzi XRNA granules colocalise with distinct mRNP granules at the nuclear periphery |
author_facet |
Costa,Jimena Ferreira da Ferrarini,Mariana Galvão Nardelli,Sheila Cristina Goldenberg,Samuel Ávila,Andréa Rodrigues Holetz,Fabíola Barbieri |
author_sort |
Costa,Jimena Ferreira da |
title |
Trypanosoma cruzi XRNA granules colocalise with distinct mRNP granules at the nuclear periphery |
title_short |
Trypanosoma cruzi XRNA granules colocalise with distinct mRNP granules at the nuclear periphery |
title_full |
Trypanosoma cruzi XRNA granules colocalise with distinct mRNP granules at the nuclear periphery |
title_fullStr |
Trypanosoma cruzi XRNA granules colocalise with distinct mRNP granules at the nuclear periphery |
title_full_unstemmed |
Trypanosoma cruzi XRNA granules colocalise with distinct mRNP granules at the nuclear periphery |
title_sort |
trypanosoma cruzi xrna granules colocalise with distinct mrnp granules at the nuclear periphery |
description |
BACKGROUND Eukaryotic ribonucleoprotein (RNP) granules are important for the regulation of RNA fate. RNP granules exist in trypanosomatids; however, their roles in controlling gene expression are still not understood. XRNA is a component of granules in Trypanosoma brucei but has not been investigated in Trypanosoma cruzi. OBJECTIVES This study aimed to investigate the TcXRNA dynamic assembly and its interaction with RNP components under conditions that affect the mRNA availability. METHODS We used in vitro metacyclogenesis of T. cruzi to observe changes in RNP granules during the differentiation process. TcXRNA expression was analysed by Western blot and immunofluorescence. Colocalisation assays were performed to investigate the interaction of TcXRNA with other RNP components. FINDINGS TcXRNA is constantly present during metacyclogenesis and is localised in cytoplasmic granules. TcXRNA does not colocalise with TcDHH1 and TcCAF1 granules in the cytoplasm. However, TcXRNA granules colocalise with mRNP granules at the nuclear periphery when mRNA processing is inhibited. MAIN CONCLUSIONS TcXRNA plays a role in mRNA metabolism as a component of mRNP granules whose assembly is dependent on mRNA availability. TcXRNA granules colocalise with distinct RNP granules at the nuclear periphery, suggesting that the perinuclear region is a regulatory compartment in T. cruzi mRNA metabolism. |
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Instituto Oswaldo Cruz, Ministério da Saúde |
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2018 |
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http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762018000600304 |
work_keys_str_mv |
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1756383887579152384 |