Performance of an in-house real-time polymerase chain reaction for identification of Mycobacterium tuberculosis isolates in laboratory routine diagnosis from a high burden setting
Abstract Brazil is one of the high burden countries for tuberculosis, and a rapid diagnosis is essential for effective control of the disease. In the present study, an in-house real-time polymerase chain reaction (PCR) assay targeting the mpt64 gene for identification of Mycobacterium tuberculosis complex isolates was evaluated under routine diagnosis conditions in a reference laboratory. From May 2011 to July 2012, 1,520 isolates of mycobacteria were prospectively submitted for phenotypic and/or PRA-hsp65 identification and to real-time PCR. The mpt64 real-time PCR showed 99.7% sensitivity and 96% specificity and detected 79.4% of the cases missed by phenotypic and PRA-hsp65 identification. The in-house real-time PCR assay showed high sensitivity and specificity and was successfully implemented in the routine diagnosis of tuberculosis in a reference laboratory from a high burden setting.
Main Authors: | Gallo,Juliana Failde, Pinhata,Juliana Maira Watanabe, Chimara,Erica, Gonçalves,Maria Gisele, Fukasawa,Lucila Okuyama, Oliveira,Rosangela Siqueira de |
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Format: | Digital revista |
Language: | English |
Published: |
Instituto Oswaldo Cruz, Ministério da Saúde
2016
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Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016000900545 |
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