Evaluation of a chemiluminescent enzyme-linked immunosorbent assay for the diagnosis of Trypanosoma cruzi infection in a nonendemic setting

The disappearance of lytic, protective antibodies (Abs) from the serum of patients with Chagas disease is accepted as a reliable indicator of parasitological cure. The efficiency of a chemiluminescent enzyme-linked immunosorbent assay based on a purified, trypomastigote-derived glycosylphosphatidylinositol-anchored mucin antigen for the serologic detection of lytic Abs against Trypanosoma cruzi was evaluated in a nonendemic setting using a panel of 92 positive and 58 negative human sera. The technique proved to be highly sensitive {100%; 95% confidence interval (CI) = 96-100} and specific (98.3%; 95% CI = 90.7-99.7), with a kappa score of 0.99. Therefore, this assay can be used to detect active T. cruzi infection and to monitor trypanosomicidal treatment.

Bibliographic Details

Main Authors:	Izquierdo,Luis, Marques,Alexandre Ferreira, Gállego,Montserrat, Sanz,Sílvia, Tebar,Sílvia, Riera,Cristina, Quintó,Llorenç, Aldasoro,Edelweiss, Almeida,Igor C, Gascon,Joaquim
Format:	Digital revista
Language:	English
Published:	Instituto Oswaldo Cruz, Ministério da Saúde 2013
Online Access:	http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000700928
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