Nylon wool column: a tool for obtaining monokine-rich eluates in the absence of serum

Nylon wool column: a tool for obtaining monokine-rich eluates in the absence of serum

Diverse conditions for stimulating human mononuclear cells to release thymocyte costimulatory factors were tested for their contribution to the generation of supernatants high titers of these monokines. Activity titers increased with LPS concentration, reaching a plateau between 1 and 10 microng/ml. Indomethacin did not modify the monokine, but the assay for thymocyte costimulatory activity was substantially affected by inhibitory substances produced by the monocytes in the absence of indomethacin. The use of nylon wool columns to trap the cells was shown to be effective in raising cellular densities without decreasing activity titers. As result, the yield per cell could be maintained even in the absence of serum, an important step toward the goal of purifiying bioactive from crude broths.

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Main Authors: Dalmau,S. R., Freitas,C. S.
Format: Digital revista
Language:English
Published: Instituto Oswaldo Cruz, Ministério da Saúde 1990
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761990000200003
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spelling oai:scielo:S0074-027619900002000032009-06-15Nylon wool column: a tool for obtaining monokine-rich eluates in the absence of serumDalmau,S. R.Freitas,C. S. nylon wool columns human monokine production thymocyte costimulatory assay LPS peripheral blood monocytes Diverse conditions for stimulating human mononuclear cells to release thymocyte costimulatory factors were tested for their contribution to the generation of supernatants high titers of these monokines. Activity titers increased with LPS concentration, reaching a plateau between 1 and 10 microng/ml. Indomethacin did not modify the monokine, but the assay for thymocyte costimulatory activity was substantially affected by inhibitory substances produced by the monocytes in the absence of indomethacin. The use of nylon wool columns to trap the cells was shown to be effective in raising cellular densities without decreasing activity titers. As result, the yield per cell could be maintained even in the absence of serum, an important step toward the goal of purifiying bioactive from crude broths.info:eu-repo/semantics/openAccessInstituto Oswaldo Cruz, Ministério da SaúdeMemórias do Instituto Oswaldo Cruz v.85 n.2 19901990-06-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761990000200003en10.1590/S0074-02761990000200003
institution SCIELO
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country Brasil
countrycode BR
component Revista
access En linea
databasecode rev-scielo-br
tag revista
region America del Sur
libraryname SciELO
language English
format Digital
author Dalmau,S. R.
Freitas,C. S.
spellingShingle Dalmau,S. R.
Freitas,C. S.
Nylon wool column: a tool for obtaining monokine-rich eluates in the absence of serum
author_facet Dalmau,S. R.
Freitas,C. S.
author_sort Dalmau,S. R.
title Nylon wool column: a tool for obtaining monokine-rich eluates in the absence of serum
title_short Nylon wool column: a tool for obtaining monokine-rich eluates in the absence of serum
title_full Nylon wool column: a tool for obtaining monokine-rich eluates in the absence of serum
title_fullStr Nylon wool column: a tool for obtaining monokine-rich eluates in the absence of serum
title_full_unstemmed Nylon wool column: a tool for obtaining monokine-rich eluates in the absence of serum
title_sort nylon wool column: a tool for obtaining monokine-rich eluates in the absence of serum
description Diverse conditions for stimulating human mononuclear cells to release thymocyte costimulatory factors were tested for their contribution to the generation of supernatants high titers of these monokines. Activity titers increased with LPS concentration, reaching a plateau between 1 and 10 microng/ml. Indomethacin did not modify the monokine, but the assay for thymocyte costimulatory activity was substantially affected by inhibitory substances produced by the monocytes in the absence of indomethacin. The use of nylon wool columns to trap the cells was shown to be effective in raising cellular densities without decreasing activity titers. As result, the yield per cell could be maintained even in the absence of serum, an important step toward the goal of purifiying bioactive from crude broths.
publisher Instituto Oswaldo Cruz, Ministério da Saúde
publishDate 1990
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761990000200003
work_keys_str_mv AT dalmausr nylonwoolcolumnatoolforobtainingmonokinericheluatesintheabsenceofserum
AT freitascs nylonwoolcolumnatoolforobtainingmonokinericheluatesintheabsenceofserum
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