Clinical and laboratory alterations in dogs naturally infected by Leishmania chagasi

Clinical and laboratory alterations in dogs naturally infected by Leishmania chagasi

INTRODUCTION: Canine visceral leishmaniasis (CVL) is a zoonotic disease with different clinical manifestations. Parasitism often occurs in bone marrow, but changes have been observed in peripheral blood and serum biochemical parameters. The aim of this study was to evaluate the hematological and biochemical parameters in dogs naturally infected by Leishmania chagasi. METHODS: Eighty-five adult dogs of both sexes and various weights and ages from the Zoonosis Control Center of Fortaleza (CCZ) were used, selected by immunofluorescence assay (IFA) and considered positive with IFA titers greater than 1:40 and by visualizing amastigotes of Leishmania chagasi in smears obtained by bone marrow aspiration. The dogs (n = 85) were grouped according to clinical signs: negative (CN = 7), subclinical (CS = 10), and clinical (CC = 68). Blood samples were collected for determination of hematological and biochemical serum values. The experimental protocol was approved by the CEUA/UECE. RESULTS: The most frequent clinical signs were cachexia (77.9%), keratitis (61.8%), and lymphadenopathy (55.9%), and 86.8% of the animals showed more than one clinical sign characteristic of CVL. In CC were observed reductions in red blood cells (63%), hematocrit (72%), and hemoglobin (62%), as well as leukocytosis (33%), neutropenia (28%), thrombocytopenia (50%), uremia (45%), hyperproteinemia (53%, p<0.05), hypergammaglobulinemia (62%, p<0.01), and hypoalbuminemia (58%). CONCLUSIONS: Animals with the clinical form of the disease demonstrate hematological and biochemical changes consistent with anemia, uremia, hyperproteinemia, and hyperglobulinemia, which present themselves as strong clinical markers of visceral leishmaniasis associated with the signs previously reported.

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Main Authors: Freitas,José Cláudio Carneiro de, Nunes-Pinheiro,Diana Célia Sousa, Lopes Neto,Belarmino Eugênio, Santos,Glauco Jonas Lemos, Abreu,Cyntia Rafaelle Amaral de, Braga,Roberta Rocha, Campos,Rafael de Morais, Oliveira,Ligene Fernandes de
Format: Digital revista
Language:English
Published: Sociedade Brasileira de Medicina Tropical - SBMT 2012
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822012000100006
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spelling oai:scielo:S0037-868220120001000062012-02-27Clinical and laboratory alterations in dogs naturally infected by Leishmania chagasiFreitas,José Cláudio Carneiro deNunes-Pinheiro,Diana Célia SousaLopes Neto,Belarmino EugênioSantos,Glauco Jonas LemosAbreu,Cyntia Rafaelle Amaral deBraga,Roberta RochaCampos,Rafael de MoraisOliveira,Ligene Fernandes de Dogs Canine visceral leishmanisis Biomarkers Anemia Uremia Hyperglobulinemia INTRODUCTION: Canine visceral leishmaniasis (CVL) is a zoonotic disease with different clinical manifestations. Parasitism often occurs in bone marrow, but changes have been observed in peripheral blood and serum biochemical parameters. The aim of this study was to evaluate the hematological and biochemical parameters in dogs naturally infected by Leishmania chagasi. METHODS: Eighty-five adult dogs of both sexes and various weights and ages from the Zoonosis Control Center of Fortaleza (CCZ) were used, selected by immunofluorescence assay (IFA) and considered positive with IFA titers greater than 1:40 and by visualizing amastigotes of Leishmania chagasi in smears obtained by bone marrow aspiration. The dogs (n = 85) were grouped according to clinical signs: negative (CN = 7), subclinical (CS = 10), and clinical (CC = 68). Blood samples were collected for determination of hematological and biochemical serum values. The experimental protocol was approved by the CEUA/UECE. RESULTS: The most frequent clinical signs were cachexia (77.9%), keratitis (61.8%), and lymphadenopathy (55.9%), and 86.8% of the animals showed more than one clinical sign characteristic of CVL. In CC were observed reductions in red blood cells (63%), hematocrit (72%), and hemoglobin (62%), as well as leukocytosis (33%), neutropenia (28%), thrombocytopenia (50%), uremia (45%), hyperproteinemia (53%, p<0.05), hypergammaglobulinemia (62%, p<0.01), and hypoalbuminemia (58%). CONCLUSIONS: Animals with the clinical form of the disease demonstrate hematological and biochemical changes consistent with anemia, uremia, hyperproteinemia, and hyperglobulinemia, which present themselves as strong clinical markers of visceral leishmaniasis associated with the signs previously reported.info:eu-repo/semantics/openAccessSociedade Brasileira de Medicina Tropical - SBMTRevista da Sociedade Brasileira de Medicina Tropical v.45 n.1 20122012-02-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822012000100006en10.1590/S0037-86822012000100006
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language English
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author Freitas,José Cláudio Carneiro de
Nunes-Pinheiro,Diana Célia Sousa
Lopes Neto,Belarmino Eugênio
Santos,Glauco Jonas Lemos
Abreu,Cyntia Rafaelle Amaral de
Braga,Roberta Rocha
Campos,Rafael de Morais
Oliveira,Ligene Fernandes de
spellingShingle Freitas,José Cláudio Carneiro de
Nunes-Pinheiro,Diana Célia Sousa
Lopes Neto,Belarmino Eugênio
Santos,Glauco Jonas Lemos
Abreu,Cyntia Rafaelle Amaral de
Braga,Roberta Rocha
Campos,Rafael de Morais
Oliveira,Ligene Fernandes de
Clinical and laboratory alterations in dogs naturally infected by Leishmania chagasi
author_facet Freitas,José Cláudio Carneiro de
Nunes-Pinheiro,Diana Célia Sousa
Lopes Neto,Belarmino Eugênio
Santos,Glauco Jonas Lemos
Abreu,Cyntia Rafaelle Amaral de
Braga,Roberta Rocha
Campos,Rafael de Morais
Oliveira,Ligene Fernandes de
author_sort Freitas,José Cláudio Carneiro de
title Clinical and laboratory alterations in dogs naturally infected by Leishmania chagasi
title_short Clinical and laboratory alterations in dogs naturally infected by Leishmania chagasi
title_full Clinical and laboratory alterations in dogs naturally infected by Leishmania chagasi
title_fullStr Clinical and laboratory alterations in dogs naturally infected by Leishmania chagasi
title_full_unstemmed Clinical and laboratory alterations in dogs naturally infected by Leishmania chagasi
title_sort clinical and laboratory alterations in dogs naturally infected by leishmania chagasi
description INTRODUCTION: Canine visceral leishmaniasis (CVL) is a zoonotic disease with different clinical manifestations. Parasitism often occurs in bone marrow, but changes have been observed in peripheral blood and serum biochemical parameters. The aim of this study was to evaluate the hematological and biochemical parameters in dogs naturally infected by Leishmania chagasi. METHODS: Eighty-five adult dogs of both sexes and various weights and ages from the Zoonosis Control Center of Fortaleza (CCZ) were used, selected by immunofluorescence assay (IFA) and considered positive with IFA titers greater than 1:40 and by visualizing amastigotes of Leishmania chagasi in smears obtained by bone marrow aspiration. The dogs (n = 85) were grouped according to clinical signs: negative (CN = 7), subclinical (CS = 10), and clinical (CC = 68). Blood samples were collected for determination of hematological and biochemical serum values. The experimental protocol was approved by the CEUA/UECE. RESULTS: The most frequent clinical signs were cachexia (77.9%), keratitis (61.8%), and lymphadenopathy (55.9%), and 86.8% of the animals showed more than one clinical sign characteristic of CVL. In CC were observed reductions in red blood cells (63%), hematocrit (72%), and hemoglobin (62%), as well as leukocytosis (33%), neutropenia (28%), thrombocytopenia (50%), uremia (45%), hyperproteinemia (53%, p<0.05), hypergammaglobulinemia (62%, p<0.01), and hypoalbuminemia (58%). CONCLUSIONS: Animals with the clinical form of the disease demonstrate hematological and biochemical changes consistent with anemia, uremia, hyperproteinemia, and hyperglobulinemia, which present themselves as strong clinical markers of visceral leishmaniasis associated with the signs previously reported.
publisher Sociedade Brasileira de Medicina Tropical - SBMT
publishDate 2012
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822012000100006
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