Genetic variability of Triatoma flavida and Triatoma bruneri (Hemiptera: Reduviidae) by RAPD-PCR technique
The Triatominae (Hemiptera:Reduviidae) contains the principal and potential Chagas disease vectors present in Mexico, Central America and South America. Triatoma flavida and T. bruneri are Cuban species. These species are closely related according to morphology and were considered synonyms until 1981, when they were separated on the grounds of external characters of the body and the morphology of male genitalia. The present study seeks to analyze genetic polymorphism of T. flavida and T. bruneri populations using RAPD techniques, and to assess the genetic relationship between these species. Ten random primers were used to evaluate the genetic variability among species using RAPD-PCR. The genetic flow among them was calculated. The dendrogram based on calculated Jaccard distances showed two clearly distinguishable clusters which coincided with the studied species. Within each species, moderate genetic differentiation (Fst 0.05-0.15) and migration rates (N > 1) were found among populations, that reveal gene flow and genetic homogeneity. Between species, the Fst value showed a high genetic differentiation and the migration rate was insufficient to maintain genetic homogeneity, and confirmed the absence of gene flow between them. Our results confirm the genetic variability among T. flavida and T. bruneri species.
Main Authors: | Fraga,Jorge, Rodriguez,Jinnay, Fuentes,Omar, Hernández,Yenin, Castex,Mayda, Gonzalez,Raul, Fernández-Calienes,Aymé |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
Instituto de Medicina Tropical de São Paulo
2011
|
Online Access: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652011000100004 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Biology of Triatoma flavida Neiva, 1911 (Hemiptera: Reduviidae) under laboratory conditions
by: Cabello,Daniel R, et al.
Published: (2001) -
Morphometric changes of Triatoma flavida Neiva, 1911 (Hemiptera:Triatominae) in the transition from sylvatic to laboratory conditions
by: Rodríguez Rodríguez,Jinnay, et al.
Published: (2007) -
Optimization of random amplified polymorphic DNA techniques for use in genetic studies of Cuban triatominae
by: Fraga,Jorge, et al.
Published: (2005) -
Triatoma patagonica (Hemiptera, Reduviidae), a New Host for Triatoma virus
by: Rozas-Dennis,Gabriela S, et al.
Published: (2002) -
Ecogenetics of Triatoma sordida and Triatoma guasayana (Hemiptera: Reduviidae) in the Bolivian Chaco
by: Noireau,François, et al.
Published: (1999)