Comparative evaluation of dry and liquid RIME LAMP in detecting trypanosomes in dead tsetse flies
Xenomonitoring is an important approach in assessing the progress of trypanosomiasis control as well as in estimating the endemicity of trypanosomes in affected areas. One of the major challenges in this approach is the unavailability of sensitive and easy to use xenomonitoring tools that can be used in the remote areas where the disease occurs. One tool that has been used successfully in detecting the parasites in tsetse flies is the repetitive insertion mobile element loop-mediated isothermal amplification (RIME LAMP). This tool has recently been modified from the liquid form to dry form for use in remote areas; however, uptake for use in the field has been slow. Field-collected tsetse flies were used to evaluate the performance of dry RIME LAMP over the conventional liquid RIME LAMP. All the samples were also subjected to internal transcribed spacer 1 (ITS1) ribosomal deoxyribonucleic acid (DNA) polymerase chain reaction (PCR) as a standard. ITS1-PCR-positive samples were further sequenced for confirmation of the species. A total of 86 wild tsetse flies were left to dry at room temperature for 3 months and DNA was extracted subsequently. All 86 flies were Glossina morsitans morsitans. From these, dry RIME LAMP detected 16.3% while liquid RIME LAMP detected 11.6% as infected with trypanosomes. Ten positive samples on ITS1-PCR were sequenced and all were shown to be trypanosomes. The use of dry RIME LAMP in the field for xenomonitoring of trypanosomes in tsetse flies will greatly contribute towards control of this neglected tropical disease as it provides the cheapest, fastest and simplest way to estimate possible human infective trypanosome infection rates in the tsetse fly vectors.
Main Authors: | , , , , , , , |
---|---|
Format: | Digital revista |
Language: | English |
Published: |
ARC-Onderstepoort Veterinary Institute - Agricultural Research Council
2018
|
Online Access: | http://www.scielo.org.za/scielo.php?script=sci_arttext&pid=S0030-24652018000100012 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
oai:scielo:S0030-24652018000100012 |
---|---|
record_format |
ojs |
spelling |
oai:scielo:S0030-246520180001000122019-01-18Comparative evaluation of dry and liquid RIME LAMP in detecting trypanosomes in dead tsetse fliesNambala,PeterMusaya,JanelisaHayashida,KyokoMaganga,EmmanuelSenga,EdwardKamoto,KelitaChisi,JohnSugimoto,ChihiroXenomonitoring is an important approach in assessing the progress of trypanosomiasis control as well as in estimating the endemicity of trypanosomes in affected areas. One of the major challenges in this approach is the unavailability of sensitive and easy to use xenomonitoring tools that can be used in the remote areas where the disease occurs. One tool that has been used successfully in detecting the parasites in tsetse flies is the repetitive insertion mobile element loop-mediated isothermal amplification (RIME LAMP). This tool has recently been modified from the liquid form to dry form for use in remote areas; however, uptake for use in the field has been slow. Field-collected tsetse flies were used to evaluate the performance of dry RIME LAMP over the conventional liquid RIME LAMP. All the samples were also subjected to internal transcribed spacer 1 (ITS1) ribosomal deoxyribonucleic acid (DNA) polymerase chain reaction (PCR) as a standard. ITS1-PCR-positive samples were further sequenced for confirmation of the species. A total of 86 wild tsetse flies were left to dry at room temperature for 3 months and DNA was extracted subsequently. All 86 flies were Glossina morsitans morsitans. From these, dry RIME LAMP detected 16.3% while liquid RIME LAMP detected 11.6% as infected with trypanosomes. Ten positive samples on ITS1-PCR were sequenced and all were shown to be trypanosomes. The use of dry RIME LAMP in the field for xenomonitoring of trypanosomes in tsetse flies will greatly contribute towards control of this neglected tropical disease as it provides the cheapest, fastest and simplest way to estimate possible human infective trypanosome infection rates in the tsetse fly vectors.ARC-Onderstepoort Veterinary Institute - Agricultural Research CouncilOnderstepoort Journal of Veterinary Research v.85 n.1 20182018-01-01journal articletext/htmlhttp://www.scielo.org.za/scielo.php?script=sci_arttext&pid=S0030-24652018000100012en |
institution |
SCIELO |
collection |
OJS |
country |
Sudáfrica |
countrycode |
ZA |
component |
Revista |
access |
En linea |
databasecode |
rev-scielo-za |
tag |
revista |
region |
África del Sur |
libraryname |
SciELO |
language |
English |
format |
Digital |
author |
Nambala,Peter Musaya,Janelisa Hayashida,Kyoko Maganga,Emmanuel Senga,Edward Kamoto,Kelita Chisi,John Sugimoto,Chihiro |
spellingShingle |
Nambala,Peter Musaya,Janelisa Hayashida,Kyoko Maganga,Emmanuel Senga,Edward Kamoto,Kelita Chisi,John Sugimoto,Chihiro Comparative evaluation of dry and liquid RIME LAMP in detecting trypanosomes in dead tsetse flies |
author_facet |
Nambala,Peter Musaya,Janelisa Hayashida,Kyoko Maganga,Emmanuel Senga,Edward Kamoto,Kelita Chisi,John Sugimoto,Chihiro |
author_sort |
Nambala,Peter |
title |
Comparative evaluation of dry and liquid RIME LAMP in detecting trypanosomes in dead tsetse flies |
title_short |
Comparative evaluation of dry and liquid RIME LAMP in detecting trypanosomes in dead tsetse flies |
title_full |
Comparative evaluation of dry and liquid RIME LAMP in detecting trypanosomes in dead tsetse flies |
title_fullStr |
Comparative evaluation of dry and liquid RIME LAMP in detecting trypanosomes in dead tsetse flies |
title_full_unstemmed |
Comparative evaluation of dry and liquid RIME LAMP in detecting trypanosomes in dead tsetse flies |
title_sort |
comparative evaluation of dry and liquid rime lamp in detecting trypanosomes in dead tsetse flies |
description |
Xenomonitoring is an important approach in assessing the progress of trypanosomiasis control as well as in estimating the endemicity of trypanosomes in affected areas. One of the major challenges in this approach is the unavailability of sensitive and easy to use xenomonitoring tools that can be used in the remote areas where the disease occurs. One tool that has been used successfully in detecting the parasites in tsetse flies is the repetitive insertion mobile element loop-mediated isothermal amplification (RIME LAMP). This tool has recently been modified from the liquid form to dry form for use in remote areas; however, uptake for use in the field has been slow. Field-collected tsetse flies were used to evaluate the performance of dry RIME LAMP over the conventional liquid RIME LAMP. All the samples were also subjected to internal transcribed spacer 1 (ITS1) ribosomal deoxyribonucleic acid (DNA) polymerase chain reaction (PCR) as a standard. ITS1-PCR-positive samples were further sequenced for confirmation of the species. A total of 86 wild tsetse flies were left to dry at room temperature for 3 months and DNA was extracted subsequently. All 86 flies were Glossina morsitans morsitans. From these, dry RIME LAMP detected 16.3% while liquid RIME LAMP detected 11.6% as infected with trypanosomes. Ten positive samples on ITS1-PCR were sequenced and all were shown to be trypanosomes. The use of dry RIME LAMP in the field for xenomonitoring of trypanosomes in tsetse flies will greatly contribute towards control of this neglected tropical disease as it provides the cheapest, fastest and simplest way to estimate possible human infective trypanosome infection rates in the tsetse fly vectors. |
publisher |
ARC-Onderstepoort Veterinary Institute - Agricultural Research Council |
publishDate |
2018 |
url |
http://www.scielo.org.za/scielo.php?script=sci_arttext&pid=S0030-24652018000100012 |
work_keys_str_mv |
AT nambalapeter comparativeevaluationofdryandliquidrimelampindetectingtrypanosomesindeadtsetseflies AT musayajanelisa comparativeevaluationofdryandliquidrimelampindetectingtrypanosomesindeadtsetseflies AT hayashidakyoko comparativeevaluationofdryandliquidrimelampindetectingtrypanosomesindeadtsetseflies AT magangaemmanuel comparativeevaluationofdryandliquidrimelampindetectingtrypanosomesindeadtsetseflies AT sengaedward comparativeevaluationofdryandliquidrimelampindetectingtrypanosomesindeadtsetseflies AT kamotokelita comparativeevaluationofdryandliquidrimelampindetectingtrypanosomesindeadtsetseflies AT chisijohn comparativeevaluationofdryandliquidrimelampindetectingtrypanosomesindeadtsetseflies AT sugimotochihiro comparativeevaluationofdryandliquidrimelampindetectingtrypanosomesindeadtsetseflies |
_version_ |
1756004625769562112 |