Memory elicitation, T-cell response and antibody production: an independent study of an inactivated entire virus vaccine (Coronavac)

Memory elicitation, T-cell response and antibody production: an independent study of an inactivated entire virus vaccine (Coronavac)

Abstract Health professionals working to mitigate the COVID-19 pandemic are one of the main risk groups for the disease, being prioritized for vaccination. Considering this, the aim of this study was to analyze the immune response of these professionals immunized with CoronaVac in the first and second doses. Blood samples were collected after the first and second doses of the vaccine (CoronaVac) and used to investigate hematological and biochemical parameters, analysis of immunoglobulin production, cytokines, and gene expression profile, as well as the identification of subsets of immune cells. Post-first dose immunological phenotypic memory (CD27+) profiles (T CD4+, TCD8+ and CD19+) showed a significant increase, as did Monocyte APCs (CD80+HLA-DR+) in relation to the second dose. The cytokines IL-2, IL-6 and IFN-° showed increased values in relation to the other analyzed cytokines. The Th2/Th17 profile in the second dose was characterized by gene expression analysis. The production of IgM and IgG after vaccination showed statistically significant values in the comparison between doses. CoronaVac showed activation of APCs monocytes, memory response of T and B lymphocytes, with immunoglobulins production. This set of responses is characterized by the Th2/Th17 immunological profile.

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Main Authors: SOUZA-SILVA,GUILHERME ANTONIO DE, AQUINO,RODRIGO CESAR A. DE, SOUSA,GEORON F. DE, BARROS,BÁRBARA RAFAELA S., LIMA,MARÍLLIA RAPHAELLA C.F. DE, CRUZ,LEONARDO C.O., MARCOS,BIANCA F. SÃO, SILVA,ANNA JÉSSICA D., TALES,APARECIDA VIRGÍNIA S., DINIZ,MADI V., LIMA,MÔNICA MARIA C., SÁ,IGOR W.A. DE, RODRIGUES,FÁBIO AUGUSTO C., SOUZA,EDSON B. DE, NASCIMENTO,ARIONE V. DO, VIEIRA,AMANDA M., CARVALHO,BRUNO M. DE, CAMPELO JÚNIOR,EVÔNIO B., FREITAS,ANTONIO CARLOS DE, MELO,CRISTIANE M.L. DE
Format: Digital revista
Language:English
Published: Academia Brasileira de Ciências 2022
Online Access:http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652022000500702
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spelling oai:scielo:S0001-376520220005007022022-06-24Memory elicitation, T-cell response and antibody production: an independent study of an inactivated entire virus vaccine (Coronavac)SOUZA-SILVA,GUILHERME ANTONIO DEAQUINO,RODRIGO CESAR A. DESOUSA,GEORON F. DEBARROS,BÁRBARA RAFAELA S.LIMA,MARÍLLIA RAPHAELLA C.F. DECRUZ,LEONARDO C.O.MARCOS,BIANCA F. SÃOSILVA,ANNA JÉSSICA D.TALES,APARECIDA VIRGÍNIA S.DINIZ,MADI V.LIMA,MÔNICA MARIA C.SÁ,IGOR W.A. DERODRIGUES,FÁBIO AUGUSTO C.SOUZA,EDSON B. DENASCIMENTO,ARIONE V. DOVIEIRA,AMANDA M.CARVALHO,BRUNO M. DECAMPELO JÚNIOR,EVÔNIO B.FREITAS,ANTONIO CARLOS DEMELO,CRISTIANE M.L. DE CoronaVac health professionals immunological memory immunoglobulin production COVID-19 SARS-CoV-2 Abstract Health professionals working to mitigate the COVID-19 pandemic are one of the main risk groups for the disease, being prioritized for vaccination. Considering this, the aim of this study was to analyze the immune response of these professionals immunized with CoronaVac in the first and second doses. Blood samples were collected after the first and second doses of the vaccine (CoronaVac) and used to investigate hematological and biochemical parameters, analysis of immunoglobulin production, cytokines, and gene expression profile, as well as the identification of subsets of immune cells. Post-first dose immunological phenotypic memory (CD27+) profiles (T CD4+, TCD8+ and CD19+) showed a significant increase, as did Monocyte APCs (CD80+HLA-DR+) in relation to the second dose. The cytokines IL-2, IL-6 and IFN-° showed increased values in relation to the other analyzed cytokines. The Th2/Th17 profile in the second dose was characterized by gene expression analysis. The production of IgM and IgG after vaccination showed statistically significant values in the comparison between doses. CoronaVac showed activation of APCs monocytes, memory response of T and B lymphocytes, with immunoglobulins production. This set of responses is characterized by the Th2/Th17 immunological profile.info:eu-repo/semantics/openAccessAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências v.94 n.3 20222022-01-01info:eu-repo/semantics/articletext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652022000500702en10.1590/0001-3765202220211590
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language English
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author SOUZA-SILVA,GUILHERME ANTONIO DE
AQUINO,RODRIGO CESAR A. DE
SOUSA,GEORON F. DE
BARROS,BÁRBARA RAFAELA S.
LIMA,MARÍLLIA RAPHAELLA C.F. DE
CRUZ,LEONARDO C.O.
MARCOS,BIANCA F. SÃO
SILVA,ANNA JÉSSICA D.
TALES,APARECIDA VIRGÍNIA S.
DINIZ,MADI V.
LIMA,MÔNICA MARIA C.
SÁ,IGOR W.A. DE
RODRIGUES,FÁBIO AUGUSTO C.
SOUZA,EDSON B. DE
NASCIMENTO,ARIONE V. DO
VIEIRA,AMANDA M.
CARVALHO,BRUNO M. DE
CAMPELO JÚNIOR,EVÔNIO B.
FREITAS,ANTONIO CARLOS DE
MELO,CRISTIANE M.L. DE
spellingShingle SOUZA-SILVA,GUILHERME ANTONIO DE
AQUINO,RODRIGO CESAR A. DE
SOUSA,GEORON F. DE
BARROS,BÁRBARA RAFAELA S.
LIMA,MARÍLLIA RAPHAELLA C.F. DE
CRUZ,LEONARDO C.O.
MARCOS,BIANCA F. SÃO
SILVA,ANNA JÉSSICA D.
TALES,APARECIDA VIRGÍNIA S.
DINIZ,MADI V.
LIMA,MÔNICA MARIA C.
SÁ,IGOR W.A. DE
RODRIGUES,FÁBIO AUGUSTO C.
SOUZA,EDSON B. DE
NASCIMENTO,ARIONE V. DO
VIEIRA,AMANDA M.
CARVALHO,BRUNO M. DE
CAMPELO JÚNIOR,EVÔNIO B.
FREITAS,ANTONIO CARLOS DE
MELO,CRISTIANE M.L. DE
Memory elicitation, T-cell response and antibody production: an independent study of an inactivated entire virus vaccine (Coronavac)
author_facet SOUZA-SILVA,GUILHERME ANTONIO DE
AQUINO,RODRIGO CESAR A. DE
SOUSA,GEORON F. DE
BARROS,BÁRBARA RAFAELA S.
LIMA,MARÍLLIA RAPHAELLA C.F. DE
CRUZ,LEONARDO C.O.
MARCOS,BIANCA F. SÃO
SILVA,ANNA JÉSSICA D.
TALES,APARECIDA VIRGÍNIA S.
DINIZ,MADI V.
LIMA,MÔNICA MARIA C.
SÁ,IGOR W.A. DE
RODRIGUES,FÁBIO AUGUSTO C.
SOUZA,EDSON B. DE
NASCIMENTO,ARIONE V. DO
VIEIRA,AMANDA M.
CARVALHO,BRUNO M. DE
CAMPELO JÚNIOR,EVÔNIO B.
FREITAS,ANTONIO CARLOS DE
MELO,CRISTIANE M.L. DE
author_sort SOUZA-SILVA,GUILHERME ANTONIO DE
title Memory elicitation, T-cell response and antibody production: an independent study of an inactivated entire virus vaccine (Coronavac)
title_short Memory elicitation, T-cell response and antibody production: an independent study of an inactivated entire virus vaccine (Coronavac)
title_full Memory elicitation, T-cell response and antibody production: an independent study of an inactivated entire virus vaccine (Coronavac)
title_fullStr Memory elicitation, T-cell response and antibody production: an independent study of an inactivated entire virus vaccine (Coronavac)
title_full_unstemmed Memory elicitation, T-cell response and antibody production: an independent study of an inactivated entire virus vaccine (Coronavac)
title_sort memory elicitation, t-cell response and antibody production: an independent study of an inactivated entire virus vaccine (coronavac)
description Abstract Health professionals working to mitigate the COVID-19 pandemic are one of the main risk groups for the disease, being prioritized for vaccination. Considering this, the aim of this study was to analyze the immune response of these professionals immunized with CoronaVac in the first and second doses. Blood samples were collected after the first and second doses of the vaccine (CoronaVac) and used to investigate hematological and biochemical parameters, analysis of immunoglobulin production, cytokines, and gene expression profile, as well as the identification of subsets of immune cells. Post-first dose immunological phenotypic memory (CD27+) profiles (T CD4+, TCD8+ and CD19+) showed a significant increase, as did Monocyte APCs (CD80+HLA-DR+) in relation to the second dose. The cytokines IL-2, IL-6 and IFN-° showed increased values in relation to the other analyzed cytokines. The Th2/Th17 profile in the second dose was characterized by gene expression analysis. The production of IgM and IgG after vaccination showed statistically significant values in the comparison between doses. CoronaVac showed activation of APCs monocytes, memory response of T and B lymphocytes, with immunoglobulins production. This set of responses is characterized by the Th2/Th17 immunological profile.
publisher Academia Brasileira de Ciências
publishDate 2022
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652022000500702
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