Is the conservation of goat semen at -150°C a viable alternative?

Is the conservation of goat semen at -150°C a viable alternative?

A series of experiments was carried out to validate a -150 °C ultra-low temperature freezer for its possible use to properly freeze and store semen. In the first part, rude sample handling was simulated to see whether temperature of stored samples was maintained within a safe range; also, the freezing point and latent heat of fusion plateau of a semen extender were monitored. In the second part, buck semen was (1) frozen in liquid nitrogen and stored in the ultra-low freezer, (2) frozen and stored in the ultra-low freezer, and (3) frozen and stored in liquid nitrogen, to compare sperm cryosurvival between freezing methods. Both, frequent removal of samples and long opening of the freezer door did not negatively affect stored samples’ temperature; latent heat of fusion plateau was five minutes long. Semen stored either at- 150 or at-196 °C. cryosurvived similarly after two days and two months of cryopreservation.

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Main Authors: Medrano, A., Cabrera, F., Gonzalez, F., Batista, M., Quesada, E., Calero, P., Gracia, A.
Format: Digital revista
Language:spa
Published: Asociacion Latinoamericana de Produccion Animal 2005
Online Access:https://ojs.alpa.uy/index.php/ojs_files/article/view/441
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spelling oai:ojs.ojs.alpa.uy:article-4412022-01-05T16:40:30Z Is the conservation of goat semen at -150°C a viable alternative? Medrano, A. Cabrera, F. Gonzalez, F. Batista, M. Quesada, E. Calero, P. Gracia, A. Goat Semen Conservation Ultra-freezer A series of experiments was carried out to validate a -150 °C ultra-low temperature freezer for its possible use to properly freeze and store semen. In the first part, rude sample handling was simulated to see whether temperature of stored samples was maintained within a safe range; also, the freezing point and latent heat of fusion plateau of a semen extender were monitored. In the second part, buck semen was (1) frozen in liquid nitrogen and stored in the ultra-low freezer, (2) frozen and stored in the ultra-low freezer, and (3) frozen and stored in liquid nitrogen, to compare sperm cryosurvival between freezing methods. Both, frequent removal of samples and long opening of the freezer door did not negatively affect stored samples’ temperature; latent heat of fusion plateau was five minutes long. Semen stored either at- 150 or at-196 °C. cryosurvived similarly after two days and two months of cryopreservation. Asociacion Latinoamericana de Produccion Animal 2005-12-10 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo cientifico original Texto application/pdf https://ojs.alpa.uy/index.php/ojs_files/article/view/441 Latin American Archives of Animal Production; Vol. 12 No. 4 (2004): Supl. 1. II Simposio Iberoamericano sobre Recursos Genéticos Archivos Latinoamericanos de Producción Animal; Vol. 12 Núm. 4 (2004): Supl. 1. II Simposio Iberoamericano sobre Recursos Genéticos 2075-8359 1022-1301 spa https://ojs.alpa.uy/index.php/ojs_files/article/view/441/391
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collection OJS
country Uruguay
countrycode UY
component Revista
access En linea
databasecode rev-alpa
tag revista
region America del Sur
libraryname Biblioteca ALPA
language spa
format Digital
author Medrano, A.
Cabrera, F.
Gonzalez, F.
Batista, M.
Quesada, E.
Calero, P.
Gracia, A.
spellingShingle Medrano, A.
Cabrera, F.
Gonzalez, F.
Batista, M.
Quesada, E.
Calero, P.
Gracia, A.
Is the conservation of goat semen at -150°C a viable alternative?
author_facet Medrano, A.
Cabrera, F.
Gonzalez, F.
Batista, M.
Quesada, E.
Calero, P.
Gracia, A.
author_sort Medrano, A.
title Is the conservation of goat semen at -150°C a viable alternative?
title_short Is the conservation of goat semen at -150°C a viable alternative?
title_full Is the conservation of goat semen at -150°C a viable alternative?
title_fullStr Is the conservation of goat semen at -150°C a viable alternative?
title_full_unstemmed Is the conservation of goat semen at -150°C a viable alternative?
title_sort is the conservation of goat semen at -150°c a viable alternative?
description A series of experiments was carried out to validate a -150 °C ultra-low temperature freezer for its possible use to properly freeze and store semen. In the first part, rude sample handling was simulated to see whether temperature of stored samples was maintained within a safe range; also, the freezing point and latent heat of fusion plateau of a semen extender were monitored. In the second part, buck semen was (1) frozen in liquid nitrogen and stored in the ultra-low freezer, (2) frozen and stored in the ultra-low freezer, and (3) frozen and stored in liquid nitrogen, to compare sperm cryosurvival between freezing methods. Both, frequent removal of samples and long opening of the freezer door did not negatively affect stored samples’ temperature; latent heat of fusion plateau was five minutes long. Semen stored either at- 150 or at-196 °C. cryosurvived similarly after two days and two months of cryopreservation.
publisher Asociacion Latinoamericana de Produccion Animal
publishDate 2005
url https://ojs.alpa.uy/index.php/ojs_files/article/view/441
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AT gonzalezf istheconservationofgoatsemenat150caviablealternative
AT batistam istheconservationofgoatsemenat150caviablealternative
AT quesadae istheconservationofgoatsemenat150caviablealternative
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