Identification of Salmonella Enteritidis and Salmonella Typhimurium in guinea pigs by the multiplex PCR

Identification of Salmonella Enteritidis and Salmonella Typhimurium in guinea pigs by the multiplex PCR

The aim of this study was to identify by mutiplex PCR-based assays the possible presence of serovars Salmonella Typhimurium and Enteritidis in 25 strains of Samonella spp that were previously isolated from guinea pigs and identified by their metabolic characteristics. The molecular analysis identified all 25 strains as Salmonella Typhimurium, evidencing the primer amplification of genes invA and fliC of Salmonella spp and Salmonella Typhimurium, respectively. This study established a rapid methodology for molecular identification of Salmonella Typhimurium and Enteritidis isolated from guinea pigs.

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Main Authors: Marcelo M., Geraldine, Rosadio A., Raúl, Chero O., Ana, Díaz O., Gerardo, Ciprian C., Aldo, Maturrano H., Lenin
Format: Digital revista
Language:spa
Published: Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria 2017
Online Access:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/13074
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spelling oai:ojs.csi.unmsm:article-130742017-09-04T21:08:14Z Identification of Salmonella Enteritidis and Salmonella Typhimurium in guinea pigs by the multiplex PCR Identificación de Salmonella Enteritidis y Salmonella Typhimurium en Cuyes mediante la Técnica de PCR Múltiple Marcelo M., Geraldine Rosadio A., Raúl Chero O., Ana Díaz O., Gerardo Ciprian C., Aldo Maturrano H., Lenin cuyes Salmonella Typhimurium Salmonella Enteritidis PCR múltiple guinea pigs Salmonella Typhimurium Salmonella Enteritidis multiplex PCR The aim of this study was to identify by mutiplex PCR-based assays the possible presence of serovars Salmonella Typhimurium and Enteritidis in 25 strains of Samonella spp that were previously isolated from guinea pigs and identified by their metabolic characteristics. The molecular analysis identified all 25 strains as Salmonella Typhimurium, evidencing the primer amplification of genes invA and fliC of Salmonella spp and Salmonella Typhimurium, respectively. This study established a rapid methodology for molecular identification of Salmonella Typhimurium and Enteritidis isolated from guinea pigs. El objetivo del presente estudio fue identificar mediante PCR múltiple la posible existencia de los serovares Salmonella Typhimurium y Enteritidis en 25 cepas de Salmonella spp previamente aisladas de cuyes e identificadas por sus características metabólicas. Mediante el análisis molecular se identificaron todas las cepas como Salmonella Typhimurium, evidenciando la amplificación de los cebadores específicos para los genes invA y fliC pertenecientes al género Salmonella y Salmonella Typhimurium, respectivamente. El presente estudio permitió establecer una metodología rápida para la identificación molecular de Salmonella Typhimurium y Enteritidis aislados de cuyes. Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria 2017-07-23 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article application/pdf https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/13074 10.15381/rivep.v28i2.13074 Revista de Investigaciones Veterinarias del Perú; Vol. 28 No. 2 (2017); 411-417 Revista de Investigaciones Veterinarias del Perú; Vol. 28 Núm. 2 (2017); 411-417 1682-3419 1609-9117 spa https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/13074/11926 Derechos de autor 2017 Geraldine Marcelo M., Raúl Rosadio A., Ana Chero O., Gerardo Díaz O., Aldo Ciprian C., Lenin Maturrano H. https://creativecommons.org/licenses/by-nc-sa/4.0
institution UNMSM
collection OJS
country Perú
countrycode PE
component Revista
access En linea
databasecode rev-rivep
tag revista
region America del Sur
libraryname Biblioteca de la Facultad de Medicina Veterinaria
language spa
format Digital
author Marcelo M., Geraldine
Rosadio A., Raúl
Chero O., Ana
Díaz O., Gerardo
Ciprian C., Aldo
Maturrano H., Lenin
spellingShingle Marcelo M., Geraldine
Rosadio A., Raúl
Chero O., Ana
Díaz O., Gerardo
Ciprian C., Aldo
Maturrano H., Lenin
Identification of Salmonella Enteritidis and Salmonella Typhimurium in guinea pigs by the multiplex PCR
author_facet Marcelo M., Geraldine
Rosadio A., Raúl
Chero O., Ana
Díaz O., Gerardo
Ciprian C., Aldo
Maturrano H., Lenin
author_sort Marcelo M., Geraldine
title Identification of Salmonella Enteritidis and Salmonella Typhimurium in guinea pigs by the multiplex PCR
title_short Identification of Salmonella Enteritidis and Salmonella Typhimurium in guinea pigs by the multiplex PCR
title_full Identification of Salmonella Enteritidis and Salmonella Typhimurium in guinea pigs by the multiplex PCR
title_fullStr Identification of Salmonella Enteritidis and Salmonella Typhimurium in guinea pigs by the multiplex PCR
title_full_unstemmed Identification of Salmonella Enteritidis and Salmonella Typhimurium in guinea pigs by the multiplex PCR
title_sort identification of salmonella enteritidis and salmonella typhimurium in guinea pigs by the multiplex pcr
description The aim of this study was to identify by mutiplex PCR-based assays the possible presence of serovars Salmonella Typhimurium and Enteritidis in 25 strains of Samonella spp that were previously isolated from guinea pigs and identified by their metabolic characteristics. The molecular analysis identified all 25 strains as Salmonella Typhimurium, evidencing the primer amplification of genes invA and fliC of Salmonella spp and Salmonella Typhimurium, respectively. This study established a rapid methodology for molecular identification of Salmonella Typhimurium and Enteritidis isolated from guinea pigs.
publisher Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
publishDate 2017
url https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/13074
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