USE OF MACROMOLECULES ON THE RATE OF IN VITRO MATURATION AND EMBRYONIC DEVELOPMENT OF BOVINE OOCYTES

USE OF MACROMOLECULES ON THE RATE OF IN VITRO MATURATION AND EMBRYONIC DEVELOPMENT OF BOVINE OOCYTES

The effect of four supplements with macromolecules on the rate of nuclear maturation, oocyte division at 48 h and embryo development at 7 days post-fertilization was evaluated. Ovaries were collected in the slaughterhouse; oocytes with two or more layers of cells were selected and matured on TCM-99 enriched with macromolecule supplements: Polyvinylpyrrolidone (PVP), polyvinylalcohol (PVA), Bovine serum albumin (BSA), Foetal bovine serum (SFB). Oocytes were cultivated at 39 °C with 5% of CO2. Nuclear maturation was evaluated at 24 hours, classifying them as germinal vesicle, metaphase I, anaphasetelophase, metaphase II and degenerated. For the evaluation of the oocyte division and embryo development, oocytes were matured under the same conditions and fertilized with spermatozoa during 18 hours in KSOM-AA culture media, and then transferred to SOF culture media for 48 h. Significant differences on nuclear maturation (Metaphase II) were only observed between PVA and SFB (p<0.05). Furthermore, none significant differences on rate of division and embryonic development between treatments were observed. These results indicate that the macromolecule supplements provide similar conditions and requirements for the progression from metaphase I to metaphase II.

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Main Authors: Santa Cruz P., Carolina, Huanca L., Wilfredo, Condori P., Rosario, Ampuero B., Antonio
Format: Digital revista
Language:spa
Published: Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria 2014
Online Access:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/10799
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spelling oai:ojs.csi.unmsm:article-107992020-03-29T23:17:33Z USE OF MACROMOLECULES ON THE RATE OF IN VITRO MATURATION AND EMBRYONIC DEVELOPMENT OF BOVINE OOCYTES Uso de Macromoléculas sobre la Tasa de Maduración y Desarrollo Embrionario in vitro de Ovocitos Bovinos Santa Cruz P., Carolina Huanca L., Wilfredo Condori P., Rosario Ampuero B., Antonio in vitro maturation bovine oocytes in vitro fecundation maduración in vitro ovocitos bovinos fecundación in vitro The effect of four supplements with macromolecules on the rate of nuclear maturation, oocyte division at 48 h and embryo development at 7 days post-fertilization was evaluated. Ovaries were collected in the slaughterhouse; oocytes with two or more layers of cells were selected and matured on TCM-99 enriched with macromolecule supplements: Polyvinylpyrrolidone (PVP), polyvinylalcohol (PVA), Bovine serum albumin (BSA), Foetal bovine serum (SFB). Oocytes were cultivated at 39 °C with 5% of CO2. Nuclear maturation was evaluated at 24 hours, classifying them as germinal vesicle, metaphase I, anaphasetelophase, metaphase II and degenerated. For the evaluation of the oocyte division and embryo development, oocytes were matured under the same conditions and fertilized with spermatozoa during 18 hours in KSOM-AA culture media, and then transferred to SOF culture media for 48 h. Significant differences on nuclear maturation (Metaphase II) were only observed between PVA and SFB (p<0.05). Furthermore, none significant differences on rate of division and embryonic development between treatments were observed. These results indicate that the macromolecule supplements provide similar conditions and requirements for the progression from metaphase I to metaphase II. Se evaluó el efecto de cuatro suplementos de macromoléculas sobre la tasa de maduración nuclear, división de ovocitos a las 48 horas y desarrollo embrionario a los siete días pos-fecundación. Se colectaron ovarios de camal, se aspiraron los folículos y se seleccionaron los ovocitos con dos o más capas de células para ser madurados en medio TCM-99 enriquecido con macromoléculas: Polivinil pirrolidona (PVP), Polivinil alcohol (PVA), Albúmina sérica bovina (BSA), Suero fetal bovino (SFB). Los ovocitos se cultivaron a 39 °C y con 5% de CO2. Se evaluó la maduración nuclear a las 24 h, calificándolos como vesícula germinal, metafase I, anafase-telofase, metafase II y degenerados. Para evaluar la división y desarrollo embrionario, otro grupo de ovocitos fueron madurados bajo las mismas condiciones y fecundados por 18 h en medio de cultivo KSOM-AA, y trasferidos al medio de cultivo SOF por 48 h. Solo hubo diferencias estadísticas en maduración nuclear (Metafase II) entre los grupos suplementados con PVA y SFB (p<0.05). Asimismo, no hubo diferencias entre tratamientos en la tasa de división y desarrollo embrionario. Los resultados indican que los suplementos de macromoléculas proporcionan condiciones y requerimientos similares para la progresión desde estadios de metafase I a metafase II. Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria 2014-12-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article application/pdf https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/10799 10.15381/rivep.v25i4.10799 Revista de Investigaciones Veterinarias del Perú; Vol. 25 No. 4 (2014); 487-493 Revista de Investigaciones Veterinarias del Perú; Vol. 25 Núm. 4 (2014); 487-493 1682-3419 1609-9117 spa https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/10799/9814 Derechos de autor 2014 Carolina Santa Cruz P., Wilfredo Huanca L., Rosario Condori P., Antonio Ampuero B. https://creativecommons.org/licenses/by-nc-sa/4.0
institution UNMSM
collection OJS
country Perú
countrycode PE
component Revista
access En linea
databasecode rev-rivep
tag revista
region America del Sur
libraryname Biblioteca de la Facultad de Medicina Veterinaria
language spa
format Digital
author Santa Cruz P., Carolina
Huanca L., Wilfredo
Condori P., Rosario
Ampuero B., Antonio
spellingShingle Santa Cruz P., Carolina
Huanca L., Wilfredo
Condori P., Rosario
Ampuero B., Antonio
USE OF MACROMOLECULES ON THE RATE OF IN VITRO MATURATION AND EMBRYONIC DEVELOPMENT OF BOVINE OOCYTES
author_facet Santa Cruz P., Carolina
Huanca L., Wilfredo
Condori P., Rosario
Ampuero B., Antonio
author_sort Santa Cruz P., Carolina
title USE OF MACROMOLECULES ON THE RATE OF IN VITRO MATURATION AND EMBRYONIC DEVELOPMENT OF BOVINE OOCYTES
title_short USE OF MACROMOLECULES ON THE RATE OF IN VITRO MATURATION AND EMBRYONIC DEVELOPMENT OF BOVINE OOCYTES
title_full USE OF MACROMOLECULES ON THE RATE OF IN VITRO MATURATION AND EMBRYONIC DEVELOPMENT OF BOVINE OOCYTES
title_fullStr USE OF MACROMOLECULES ON THE RATE OF IN VITRO MATURATION AND EMBRYONIC DEVELOPMENT OF BOVINE OOCYTES
title_full_unstemmed USE OF MACROMOLECULES ON THE RATE OF IN VITRO MATURATION AND EMBRYONIC DEVELOPMENT OF BOVINE OOCYTES
title_sort use of macromolecules on the rate of in vitro maturation and embryonic development of bovine oocytes
description The effect of four supplements with macromolecules on the rate of nuclear maturation, oocyte division at 48 h and embryo development at 7 days post-fertilization was evaluated. Ovaries were collected in the slaughterhouse; oocytes with two or more layers of cells were selected and matured on TCM-99 enriched with macromolecule supplements: Polyvinylpyrrolidone (PVP), polyvinylalcohol (PVA), Bovine serum albumin (BSA), Foetal bovine serum (SFB). Oocytes were cultivated at 39 °C with 5% of CO2. Nuclear maturation was evaluated at 24 hours, classifying them as germinal vesicle, metaphase I, anaphasetelophase, metaphase II and degenerated. For the evaluation of the oocyte division and embryo development, oocytes were matured under the same conditions and fertilized with spermatozoa during 18 hours in KSOM-AA culture media, and then transferred to SOF culture media for 48 h. Significant differences on nuclear maturation (Metaphase II) were only observed between PVA and SFB (p<0.05). Furthermore, none significant differences on rate of division and embryonic development between treatments were observed. These results indicate that the macromolecule supplements provide similar conditions and requirements for the progression from metaphase I to metaphase II.
publisher Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
publishDate 2014
url https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/10799
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AT huancalwilfredo useofmacromoleculesontherateofinvitromaturationandembryonicdevelopmentofbovineoocytes
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