Comparison of 3 culture methods and PCR assays for Salmonella gallinarum and Salmonella pullorum detection in poultry feed
To detect Salmonella gallinarum or Salmonella pullorum in artificially contaminated poultry feed, 9 culture combinations were compared, including 3 preenrichment/enrichment methods (tryptic soy broth plus ferrous sulfate/tetrathionate Hajna, tryptic soy broth plus ferrous sulfate/selenite cystine broth, and Salmosyst) in combination with 3 selective agars (xylose lysine desoxicholate agar added with tergitol 4, EF-18, and Önöz), respectively. Additionally, a single PCR technique was applied combined with 2 different preenrichment media (tryptic soy broth plus ferrous sulfate and Salmosyst). The specificity and positive predictive value were 1 for all methods. There were some differences among Salmonella strains for sensitivity and accuracy in the culture and Salmosyst-PCR methods. The sensitivity and accuracy values were less than 0.60 and 0.64, respectively, whereas the negative predictive values were between 0.12 and 0.23. Two PCR methods did not show any difference in the parameters of performance evaluated. Kappa coefficients showed good agreement between both methods. None of the culture combinations was able to detect S. gallinarum or S. pullorum when the inoculum was less than 3 × 102 cfu/25 g, except the Salmosyst broth method, which could recover S. gallinarum from 3 × 101 cfu/25 g onward. Overall, there were differences in the detection limits among the strains and methods used. In general, the 3 selective plating media did not show any significant difference in the parameters of performance studied for each strain. On the other hand, the agreements were slight to fair when culture methods were compared among them and with both PCR methods. The differences in the detection levels that were obtained using these methods and the difficulty in detecting S. gallinarum or S. pullorum in feed represent a potential problem when a poultry feed sample is considered to be negative. It is highly recommended to use at least 2 methods to increase the chances of detecting S. gallinarum or S. pullorum in poultry feed.
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Oxford Academic Press
2013-06
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Subjects: | Enfermedades de los Animales, Aves de Corral, Salmonella gallinarum, Salmonella pullorum, PCR, Técnicas de Cultivo, Alimentación Avícola, Animal Diseases, Poultry, Culture Techniques, Poultry Feeding, |
Online Access: | https://academic.oup.com/ps/article/92/6/1505/1592721 http://hdl.handle.net/20.500.12123/6463 https://doi.org/10.3382/ps.2012-02926 |
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oai:localhost:20.500.12123-64632019-12-06T12:15:38Z Comparison of 3 culture methods and PCR assays for Salmonella gallinarum and Salmonella pullorum detection in poultry feed Soria, Maria Cecilia Soria, Mario Bueno, Dante Javier Terzolo, Horacio Enfermedades de los Animales Aves de Corral Salmonella gallinarum Salmonella pullorum PCR Técnicas de Cultivo Alimentación Avícola Animal Diseases Poultry Culture Techniques Poultry Feeding To detect Salmonella gallinarum or Salmonella pullorum in artificially contaminated poultry feed, 9 culture combinations were compared, including 3 preenrichment/enrichment methods (tryptic soy broth plus ferrous sulfate/tetrathionate Hajna, tryptic soy broth plus ferrous sulfate/selenite cystine broth, and Salmosyst) in combination with 3 selective agars (xylose lysine desoxicholate agar added with tergitol 4, EF-18, and Önöz), respectively. Additionally, a single PCR technique was applied combined with 2 different preenrichment media (tryptic soy broth plus ferrous sulfate and Salmosyst). The specificity and positive predictive value were 1 for all methods. There were some differences among Salmonella strains for sensitivity and accuracy in the culture and Salmosyst-PCR methods. The sensitivity and accuracy values were less than 0.60 and 0.64, respectively, whereas the negative predictive values were between 0.12 and 0.23. Two PCR methods did not show any difference in the parameters of performance evaluated. Kappa coefficients showed good agreement between both methods. None of the culture combinations was able to detect S. gallinarum or S. pullorum when the inoculum was less than 3 × 102 cfu/25 g, except the Salmosyst broth method, which could recover S. gallinarum from 3 × 101 cfu/25 g onward. Overall, there were differences in the detection limits among the strains and methods used. In general, the 3 selective plating media did not show any significant difference in the parameters of performance studied for each strain. On the other hand, the agreements were slight to fair when culture methods were compared among them and with both PCR methods. The differences in the detection levels that were obtained using these methods and the difficulty in detecting S. gallinarum or S. pullorum in feed represent a potential problem when a poultry feed sample is considered to be negative. It is highly recommended to use at least 2 methods to increase the chances of detecting S. gallinarum or S. pullorum in poultry feed. EEA Concepción del Uruguay Fil: Soria, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Soria, Mario Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina Fil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina Fil: Terzolo, Horacio Raúl. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. 2019-12-06T11:59:16Z 2019-12-06T11:59:16Z 2013-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://academic.oup.com/ps/article/92/6/1505/1592721 http://hdl.handle.net/20.500.12123/6463 0032-5791 1525-3171 https://doi.org/10.3382/ps.2012-02926 eng info:eu-repo/semantics/openAccess application/pdf Oxford Academic Press Poultry Science 92 (6) : 1505–1515 (June 2013) |
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Enfermedades de los Animales Aves de Corral Salmonella gallinarum Salmonella pullorum PCR Técnicas de Cultivo Alimentación Avícola Animal Diseases Poultry Culture Techniques Poultry Feeding Enfermedades de los Animales Aves de Corral Salmonella gallinarum Salmonella pullorum PCR Técnicas de Cultivo Alimentación Avícola Animal Diseases Poultry Culture Techniques Poultry Feeding |
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Enfermedades de los Animales Aves de Corral Salmonella gallinarum Salmonella pullorum PCR Técnicas de Cultivo Alimentación Avícola Animal Diseases Poultry Culture Techniques Poultry Feeding Enfermedades de los Animales Aves de Corral Salmonella gallinarum Salmonella pullorum PCR Técnicas de Cultivo Alimentación Avícola Animal Diseases Poultry Culture Techniques Poultry Feeding Soria, Maria Cecilia Soria, Mario Bueno, Dante Javier Terzolo, Horacio Comparison of 3 culture methods and PCR assays for Salmonella gallinarum and Salmonella pullorum detection in poultry feed |
description |
To detect Salmonella gallinarum or Salmonella pullorum in artificially contaminated poultry feed, 9 culture combinations were compared, including 3 preenrichment/enrichment methods (tryptic soy broth plus ferrous sulfate/tetrathionate Hajna, tryptic soy broth plus ferrous sulfate/selenite cystine broth, and Salmosyst) in combination with 3 selective agars (xylose lysine desoxicholate agar added with tergitol 4, EF-18, and Önöz), respectively. Additionally, a single PCR technique was applied combined with 2 different preenrichment media (tryptic soy broth plus ferrous sulfate and Salmosyst). The specificity and positive predictive value were 1 for all methods. There were some differences among Salmonella strains for sensitivity and accuracy in the culture and Salmosyst-PCR methods. The sensitivity and accuracy values were less than 0.60 and 0.64, respectively, whereas the negative predictive values were between 0.12 and 0.23. Two PCR methods did not show any difference in the parameters of performance evaluated. Kappa coefficients showed good agreement between both methods. None of the culture combinations was able to detect S. gallinarum or S. pullorum when the inoculum was less than 3 × 102 cfu/25 g, except the Salmosyst broth method, which could recover S. gallinarum from 3 × 101 cfu/25 g onward. Overall, there were differences in the detection limits among the strains and methods used. In general, the 3 selective plating media did not show any significant difference in the parameters of performance studied for each strain. On the other hand, the agreements were slight to fair when culture methods were compared among them and with both PCR methods. The differences in the detection levels that were obtained using these methods and the difficulty in detecting S. gallinarum or S. pullorum in feed represent a potential problem when a poultry feed sample is considered to be negative. It is highly recommended to use at least 2 methods to increase the chances of detecting S. gallinarum or S. pullorum in poultry feed. |
format |
info:ar-repo/semantics/artículo |
topic_facet |
Enfermedades de los Animales Aves de Corral Salmonella gallinarum Salmonella pullorum PCR Técnicas de Cultivo Alimentación Avícola Animal Diseases Poultry Culture Techniques Poultry Feeding |
author |
Soria, Maria Cecilia Soria, Mario Bueno, Dante Javier Terzolo, Horacio |
author_facet |
Soria, Maria Cecilia Soria, Mario Bueno, Dante Javier Terzolo, Horacio |
author_sort |
Soria, Maria Cecilia |
title |
Comparison of 3 culture methods and PCR assays for Salmonella gallinarum and Salmonella pullorum detection in poultry feed |
title_short |
Comparison of 3 culture methods and PCR assays for Salmonella gallinarum and Salmonella pullorum detection in poultry feed |
title_full |
Comparison of 3 culture methods and PCR assays for Salmonella gallinarum and Salmonella pullorum detection in poultry feed |
title_fullStr |
Comparison of 3 culture methods and PCR assays for Salmonella gallinarum and Salmonella pullorum detection in poultry feed |
title_full_unstemmed |
Comparison of 3 culture methods and PCR assays for Salmonella gallinarum and Salmonella pullorum detection in poultry feed |
title_sort |
comparison of 3 culture methods and pcr assays for salmonella gallinarum and salmonella pullorum detection in poultry feed |
publisher |
Oxford Academic Press |
publishDate |
2013-06 |
url |
https://academic.oup.com/ps/article/92/6/1505/1592721 http://hdl.handle.net/20.500.12123/6463 https://doi.org/10.3382/ps.2012-02926 |
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