Minimally processed crude leaf extracts of Nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in mice
Foot-and-mouth disease (FMD) remains one of the most feared viral diseases affecting cloven-hoofed animals, and results in severe economic losses. Currently available vaccines are based on inactivated FMD virus (FMDV). The use of recombinant FMDV-like particles (VLPs) as subunit vaccines has gained importance because of their immunogenic properties and safety. We evaluated the production of FMD VLPs, via Agrobacterium-mediated transient expression, and the immunogenicity of these structures in mice. Leaves were infiltrated with pEAQ-HT and pRIC 3.0 vectors encoding the capsid precursor P1-2A and the protease 3C. The recombinant protein yield was 3–4 mg/kg of fresh leaf tissue. Both groups of mice immunized with purified VLPs and mice immunized with the crude leaf extract elicited a specific humoral response with similar antibody titers. Thus, minimally processed plant material containing transiently expressed FMD VLPs could be a scalable and cost-effective technology for the production of a recombinant subunit vaccine against FMDV.
| Main Authors: | , , , , |
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| Format: | info:ar-repo/semantics/artículo biblioteca |
| Language: | eng |
| Published: |
Elsevier
2018-12
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| Subjects: | Extractos de Hoja, Virus Fiebre Aftosa, Inmunogenética, Ratón, Leaf Extracts, Aphthovirus, immunogenetics, Mice, Nicotiana Benthamiana, Foot and Mouth Disease Virus, |
| Online Access: | http://hdl.handle.net/20.500.12123/4443 https://www.sciencedirect.com/science/article/pii/S2215017X1830050X?via%3Dihub https://doi.org/10.1016/j.btre.2018.e00283 |
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