Efficacy of immunization with a recombinant S. aureus vaccine formulated with liposomes and ODN-CpG against natural S. aureus intramammary infections in heifers and cows
The aims of the present study were to evaluate the ability of a subunit vaccine composed of recombinant molecules of α-toxin, β-toxin, FnBPA and ClfA, formulated with cationic liposomes and CpG-ODN, to confer protection against natural S. aureus intramammary infection (IMI) and to assess the antibody response against the vaccine components. A stringent criterion based on molecular identification of the isolates was used to define IMI. The proportion of animals that developed new S. aureus IMI was higher in the Control group compared with the Vaccine group (reduction of 60.7%), and time to new S. aureus IMI was higher for animals in the Vaccine group compared with animals in the Control group, although not statistically significant. Molecular identification of the isolates allowed the detection of S. aureus pulsotypes that appeared transiently in milk and others that were able to establish IMI, providing a new perspective to define parameters related to the definition of new IMI and cures. Specific IgG, IgG1 and IgG2 levels against the four recombinant proteins included in the vaccine were significantly increased in the vaccinated group and the recombinant α-toxin included in the vaccine generated antibodies that reduced significantly the haemolytic activity of native α-toxin. Data reported in the present study indicate a possible effect on both the proportion of animals developing new IMI and the time to new S. aureus IMI, but the incidence of disease within the study was too low to provide statistical confirmation.
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Elsevier
2022-03-07T14:02:23Z
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Subjects: | Novilla, Vaca, Staphylococcus aureus, Inmunización, Vacuna, Proteínas Recombinantes, Heifers, Cows, Immunization, Vaccines, Bovine Mastitis, Recombinant Proteins, Mastítis Bovina, |
Online Access: | http://hdl.handle.net/20.500.12123/11331 https://www.sciencedirect.com/science/article/pii/S0034528822000510 https://doi.org/10.1016/j.rvsc.2022.02.014 |
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Novilla Vaca Staphylococcus aureus Inmunización Vacuna Proteínas Recombinantes Heifers Cows Immunization Vaccines Bovine Mastitis Recombinant Proteins Mastítis Bovina Novilla Vaca Staphylococcus aureus Inmunización Vacuna Proteínas Recombinantes Heifers Cows Immunization Vaccines Bovine Mastitis Recombinant Proteins Mastítis Bovina |
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Novilla Vaca Staphylococcus aureus Inmunización Vacuna Proteínas Recombinantes Heifers Cows Immunization Vaccines Bovine Mastitis Recombinant Proteins Mastítis Bovina Novilla Vaca Staphylococcus aureus Inmunización Vacuna Proteínas Recombinantes Heifers Cows Immunization Vaccines Bovine Mastitis Recombinant Proteins Mastítis Bovina Camussone, Cecilia Reidel, Ivana Gabriela Molineri, Ana Ines Cicotello, Joaquin Miotti, Camila Suarez Archilla, Guillermo Curti, C.C. Veaute, Carolina Melania Isabel Calvinho, Luis Fernando Efficacy of immunization with a recombinant S. aureus vaccine formulated with liposomes and ODN-CpG against natural S. aureus intramammary infections in heifers and cows |
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The aims of the present study were to evaluate the ability of a subunit vaccine composed of recombinant molecules of α-toxin, β-toxin, FnBPA and ClfA, formulated with cationic liposomes and CpG-ODN, to confer protection against natural S. aureus intramammary infection (IMI) and to assess the antibody response against the vaccine components. A stringent criterion based on molecular identification of the isolates was used to define IMI. The proportion of animals that developed new S. aureus IMI was higher in the Control group compared with the Vaccine group (reduction of 60.7%), and time to new S. aureus IMI was higher for animals in the Vaccine group compared with animals in the Control group, although not statistically significant. Molecular identification of the isolates allowed the detection of S. aureus pulsotypes that appeared transiently in milk and others that were able to establish IMI, providing a new perspective to define parameters related to the definition of new IMI and cures. Specific IgG, IgG1 and IgG2 levels against the four recombinant proteins included in the vaccine were significantly increased in the vaccinated group and the recombinant α-toxin included in the vaccine generated antibodies that reduced significantly the haemolytic activity of native α-toxin. Data reported in the present study indicate a possible effect on both the proportion of animals developing new IMI and the time to new S. aureus IMI, but the incidence of disease within the study was too low to provide statistical confirmation. |
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Novilla Vaca Staphylococcus aureus Inmunización Vacuna Proteínas Recombinantes Heifers Cows Immunization Vaccines Bovine Mastitis Recombinant Proteins Mastítis Bovina |
author |
Camussone, Cecilia Reidel, Ivana Gabriela Molineri, Ana Ines Cicotello, Joaquin Miotti, Camila Suarez Archilla, Guillermo Curti, C.C. Veaute, Carolina Melania Isabel Calvinho, Luis Fernando |
author_facet |
Camussone, Cecilia Reidel, Ivana Gabriela Molineri, Ana Ines Cicotello, Joaquin Miotti, Camila Suarez Archilla, Guillermo Curti, C.C. Veaute, Carolina Melania Isabel Calvinho, Luis Fernando |
author_sort |
Camussone, Cecilia |
title |
Efficacy of immunization with a recombinant S. aureus vaccine formulated with liposomes and ODN-CpG against natural S. aureus intramammary infections in heifers and cows |
title_short |
Efficacy of immunization with a recombinant S. aureus vaccine formulated with liposomes and ODN-CpG against natural S. aureus intramammary infections in heifers and cows |
title_full |
Efficacy of immunization with a recombinant S. aureus vaccine formulated with liposomes and ODN-CpG against natural S. aureus intramammary infections in heifers and cows |
title_fullStr |
Efficacy of immunization with a recombinant S. aureus vaccine formulated with liposomes and ODN-CpG against natural S. aureus intramammary infections in heifers and cows |
title_full_unstemmed |
Efficacy of immunization with a recombinant S. aureus vaccine formulated with liposomes and ODN-CpG against natural S. aureus intramammary infections in heifers and cows |
title_sort |
efficacy of immunization with a recombinant s. aureus vaccine formulated with liposomes and odn-cpg against natural s. aureus intramammary infections in heifers and cows |
publisher |
Elsevier |
publishDate |
2022-03-07T14:02:23Z |
url |
http://hdl.handle.net/20.500.12123/11331 https://www.sciencedirect.com/science/article/pii/S0034528822000510 https://doi.org/10.1016/j.rvsc.2022.02.014 |
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oai:localhost:20.500.12123-113312022-04-01T14:34:02Z Efficacy of immunization with a recombinant S. aureus vaccine formulated with liposomes and ODN-CpG against natural S. aureus intramammary infections in heifers and cows Camussone, Cecilia Reidel, Ivana Gabriela Molineri, Ana Ines Cicotello, Joaquin Miotti, Camila Suarez Archilla, Guillermo Curti, C.C. Veaute, Carolina Melania Isabel Calvinho, Luis Fernando Novilla Vaca Staphylococcus aureus Inmunización Vacuna Proteínas Recombinantes Heifers Cows Immunization Vaccines Bovine Mastitis Recombinant Proteins Mastítis Bovina The aims of the present study were to evaluate the ability of a subunit vaccine composed of recombinant molecules of α-toxin, β-toxin, FnBPA and ClfA, formulated with cationic liposomes and CpG-ODN, to confer protection against natural S. aureus intramammary infection (IMI) and to assess the antibody response against the vaccine components. A stringent criterion based on molecular identification of the isolates was used to define IMI. The proportion of animals that developed new S. aureus IMI was higher in the Control group compared with the Vaccine group (reduction of 60.7%), and time to new S. aureus IMI was higher for animals in the Vaccine group compared with animals in the Control group, although not statistically significant. Molecular identification of the isolates allowed the detection of S. aureus pulsotypes that appeared transiently in milk and others that were able to establish IMI, providing a new perspective to define parameters related to the definition of new IMI and cures. Specific IgG, IgG1 and IgG2 levels against the four recombinant proteins included in the vaccine were significantly increased in the vaccinated group and the recombinant α-toxin included in the vaccine generated antibodies that reduced significantly the haemolytic activity of native α-toxin. Data reported in the present study indicate a possible effect on both the proportion of animals developing new IMI and the time to new S. aureus IMI, but the incidence of disease within the study was too low to provide statistical confirmation. EEA Rafaela Fil: Camussone, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Camussone, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Reidel, Ivana Gabriela. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Inmunología Experimental; Argentina Fil: Molineri, Ana Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Fil: Molineri, Ana Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cicotello, Joaquín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Cicotello, Joaquín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Miotti, Camila. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Miotti, Camila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Suarez Archilla, Guillermo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Suarez Archilla, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Curti, C.C. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Inmunología Experimental; Argentina Fil: Veaute, Carolina Melania Isabel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Inmunología Experimental; Argentina. Fil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Calvinho, Luis Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina info:eu-repo/date/embargoEnd/2023-03-07 2022-03-07T14:02:23Z 2022-03-07T14:02:23Z 2022-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/11331 https://www.sciencedirect.com/science/article/pii/S0034528822000510 0034-5288 https://doi.org/10.1016/j.rvsc.2022.02.014 eng info:eu-repograntAgreement/INTA/2019-PD-E5-I102-001/2019-PD-E5-I102-001/AR./Desarrollo de vacunas y tecnologías para mejorar las estrategias profilácticas y terapéuticas de las enfermedades que afectan la producción animal y la salud pública info:eu-repo/semantics/embargoedAccess application/pdf Elsevier Research in Veterinary Science 145 : 177-187 (July 2022) |