An Autophagy-Related Kinase Is Essential for the Symbiotic Relationship between Phaseolus vulgaris and Both Rhizobia and Arbuscular Mycorrhizal Fungi
Eukaryotes contain three types of lipid kinases that belong to the phosphatidylinositol 3-kinase (PI3K) family. In plants and Saccharomyces cerevisiae, only PI3K class III family members have been identified. These enzymes regulate the innate immune response, intracellular trafficking, autophagy, and senescence. Here, we report that RNAi-mediated downregulation of common bean (Phaseolus vulgaris) PI3K severely impaired symbiosis in composite P. vulgaris plants with endosymbionts such as Rhizobium tropici and Rhizophagus irregularis. Downregulation of Pv-PI3K was associated with a marked decrease in root hair growth and curling. Additionally, infection thread growth, root-nodule number, and symbiosome formation in root nodule cells were severely affected. Interestingly, root colonization by AM fungi and the formation of arbuscules were also abolished in PI3K loss-of-function plants. Furthermore, the transcript accumulation of genes encoding proteins known to interact with PI3K to form protein complexes involved in autophagy was drastically reduced in these transgenic roots. RNAi-mediated downregulation of one of these genes, Beclin1/Atg6, resulted in a similar phenotype as observed for transgenic roots in which Pv-PI3K had been downregulated. Our findings show that an autophagy-related process is crucial for the mutualistic interactions of P. vulgaris with beneficial microorganisms
Main Authors: | , , , , , , , , , , |
---|---|
Format: | info:ar-repo/semantics/artículo biblioteca |
Language: | eng |
Published: |
American Society of Plant Biologists
2016-09
|
Subjects: | Phaseolus Vulgaris, Rhizobiaceae, Arbuscular Mycorrhiza, Kinases, Micorrizas Arbusculares, Quinasas, Poroto, |
Online Access: | http://hdl.handle.net/20.500.12123/10954 https://academic.oup.com/plcell/article/28/9/2326/6098387 https://doi.org/10.1105/tpc.15.01012 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
oai:localhost:20.500.12123-10954 |
---|---|
record_format |
koha |
institution |
INTA AR |
collection |
DSpace |
country |
Argentina |
countrycode |
AR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-inta-ar |
tag |
biblioteca |
region |
America del Sur |
libraryname |
Biblioteca Central del INTA Argentina |
language |
eng |
topic |
Phaseolus Vulgaris Rhizobiaceae Arbuscular Mycorrhiza Kinases Micorrizas Arbusculares Quinasas Poroto Phaseolus Vulgaris Rhizobiaceae Arbuscular Mycorrhiza Kinases Micorrizas Arbusculares Quinasas Poroto |
spellingShingle |
Phaseolus Vulgaris Rhizobiaceae Arbuscular Mycorrhiza Kinases Micorrizas Arbusculares Quinasas Poroto Phaseolus Vulgaris Rhizobiaceae Arbuscular Mycorrhiza Kinases Micorrizas Arbusculares Quinasas Poroto Estrada-Navarrete, Georgina Cruz-Mireles, Neftaly Lascano, Hernan Ramiro Alvarado-Affantranger, Xóchitl Hernández-Barrera, Alejandra Barraza, Aarón Olivares, Juan E. Arthikala, Manoj-Kumar Cárdenas, Luis Quinto, Carmen Sanchez, Federico An Autophagy-Related Kinase Is Essential for the Symbiotic Relationship between Phaseolus vulgaris and Both Rhizobia and Arbuscular Mycorrhizal Fungi |
description |
Eukaryotes contain three types of lipid kinases that belong to the phosphatidylinositol 3-kinase (PI3K) family. In plants and Saccharomyces cerevisiae, only PI3K class III family members have been identified. These enzymes regulate the innate immune response, intracellular trafficking, autophagy, and senescence. Here, we report that RNAi-mediated downregulation of common bean (Phaseolus vulgaris) PI3K severely impaired symbiosis in composite P. vulgaris plants with endosymbionts such as Rhizobium tropici and Rhizophagus irregularis. Downregulation of Pv-PI3K was associated with a marked decrease in root hair growth and curling. Additionally, infection thread growth, root-nodule number, and symbiosome formation in root nodule cells were severely affected. Interestingly, root colonization by AM fungi and the formation of arbuscules were also abolished in PI3K loss-of-function plants. Furthermore, the transcript accumulation of genes encoding proteins known to interact with PI3K to form protein complexes involved in autophagy was drastically reduced in these transgenic roots. RNAi-mediated downregulation of one of these genes, Beclin1/Atg6, resulted in a similar phenotype as observed for transgenic roots in which Pv-PI3K had been downregulated. Our findings show that an autophagy-related process is crucial for the mutualistic interactions of P. vulgaris with beneficial microorganisms |
format |
info:ar-repo/semantics/artículo |
topic_facet |
Phaseolus Vulgaris Rhizobiaceae Arbuscular Mycorrhiza Kinases Micorrizas Arbusculares Quinasas Poroto |
author |
Estrada-Navarrete, Georgina Cruz-Mireles, Neftaly Lascano, Hernan Ramiro Alvarado-Affantranger, Xóchitl Hernández-Barrera, Alejandra Barraza, Aarón Olivares, Juan E. Arthikala, Manoj-Kumar Cárdenas, Luis Quinto, Carmen Sanchez, Federico |
author_facet |
Estrada-Navarrete, Georgina Cruz-Mireles, Neftaly Lascano, Hernan Ramiro Alvarado-Affantranger, Xóchitl Hernández-Barrera, Alejandra Barraza, Aarón Olivares, Juan E. Arthikala, Manoj-Kumar Cárdenas, Luis Quinto, Carmen Sanchez, Federico |
author_sort |
Estrada-Navarrete, Georgina |
title |
An Autophagy-Related Kinase Is Essential for the Symbiotic Relationship between Phaseolus vulgaris and Both Rhizobia and Arbuscular Mycorrhizal Fungi |
title_short |
An Autophagy-Related Kinase Is Essential for the Symbiotic Relationship between Phaseolus vulgaris and Both Rhizobia and Arbuscular Mycorrhizal Fungi |
title_full |
An Autophagy-Related Kinase Is Essential for the Symbiotic Relationship between Phaseolus vulgaris and Both Rhizobia and Arbuscular Mycorrhizal Fungi |
title_fullStr |
An Autophagy-Related Kinase Is Essential for the Symbiotic Relationship between Phaseolus vulgaris and Both Rhizobia and Arbuscular Mycorrhizal Fungi |
title_full_unstemmed |
An Autophagy-Related Kinase Is Essential for the Symbiotic Relationship between Phaseolus vulgaris and Both Rhizobia and Arbuscular Mycorrhizal Fungi |
title_sort |
autophagy-related kinase is essential for the symbiotic relationship between phaseolus vulgaris and both rhizobia and arbuscular mycorrhizal fungi |
publisher |
American Society of Plant Biologists |
publishDate |
2016-09 |
url |
http://hdl.handle.net/20.500.12123/10954 https://academic.oup.com/plcell/article/28/9/2326/6098387 https://doi.org/10.1105/tpc.15.01012 |
work_keys_str_mv |
AT estradanavarretegeorgina anautophagyrelatedkinaseisessentialforthesymbioticrelationshipbetweenphaseolusvulgarisandbothrhizobiaandarbuscularmycorrhizalfungi AT cruzmirelesneftaly anautophagyrelatedkinaseisessentialforthesymbioticrelationshipbetweenphaseolusvulgarisandbothrhizobiaandarbuscularmycorrhizalfungi AT lascanohernanramiro anautophagyrelatedkinaseisessentialforthesymbioticrelationshipbetweenphaseolusvulgarisandbothrhizobiaandarbuscularmycorrhizalfungi AT alvaradoaffantrangerxochitl anautophagyrelatedkinaseisessentialforthesymbioticrelationshipbetweenphaseolusvulgarisandbothrhizobiaandarbuscularmycorrhizalfungi AT hernandezbarreraalejandra anautophagyrelatedkinaseisessentialforthesymbioticrelationshipbetweenphaseolusvulgarisandbothrhizobiaandarbuscularmycorrhizalfungi AT barrazaaaron anautophagyrelatedkinaseisessentialforthesymbioticrelationshipbetweenphaseolusvulgarisandbothrhizobiaandarbuscularmycorrhizalfungi AT olivaresjuane anautophagyrelatedkinaseisessentialforthesymbioticrelationshipbetweenphaseolusvulgarisandbothrhizobiaandarbuscularmycorrhizalfungi AT arthikalamanojkumar anautophagyrelatedkinaseisessentialforthesymbioticrelationshipbetweenphaseolusvulgarisandbothrhizobiaandarbuscularmycorrhizalfungi AT cardenasluis anautophagyrelatedkinaseisessentialforthesymbioticrelationshipbetweenphaseolusvulgarisandbothrhizobiaandarbuscularmycorrhizalfungi AT quintocarmen anautophagyrelatedkinaseisessentialforthesymbioticrelationshipbetweenphaseolusvulgarisandbothrhizobiaandarbuscularmycorrhizalfungi AT sanchezfederico anautophagyrelatedkinaseisessentialforthesymbioticrelationshipbetweenphaseolusvulgarisandbothrhizobiaandarbuscularmycorrhizalfungi AT estradanavarretegeorgina autophagyrelatedkinaseisessentialforthesymbioticrelationshipbetweenphaseolusvulgarisandbothrhizobiaandarbuscularmycorrhizalfungi AT cruzmirelesneftaly autophagyrelatedkinaseisessentialforthesymbioticrelationshipbetweenphaseolusvulgarisandbothrhizobiaandarbuscularmycorrhizalfungi AT lascanohernanramiro autophagyrelatedkinaseisessentialforthesymbioticrelationshipbetweenphaseolusvulgarisandbothrhizobiaandarbuscularmycorrhizalfungi AT alvaradoaffantrangerxochitl autophagyrelatedkinaseisessentialforthesymbioticrelationshipbetweenphaseolusvulgarisandbothrhizobiaandarbuscularmycorrhizalfungi AT hernandezbarreraalejandra autophagyrelatedkinaseisessentialforthesymbioticrelationshipbetweenphaseolusvulgarisandbothrhizobiaandarbuscularmycorrhizalfungi AT barrazaaaron autophagyrelatedkinaseisessentialforthesymbioticrelationshipbetweenphaseolusvulgarisandbothrhizobiaandarbuscularmycorrhizalfungi AT olivaresjuane autophagyrelatedkinaseisessentialforthesymbioticrelationshipbetweenphaseolusvulgarisandbothrhizobiaandarbuscularmycorrhizalfungi AT arthikalamanojkumar autophagyrelatedkinaseisessentialforthesymbioticrelationshipbetweenphaseolusvulgarisandbothrhizobiaandarbuscularmycorrhizalfungi AT cardenasluis autophagyrelatedkinaseisessentialforthesymbioticrelationshipbetweenphaseolusvulgarisandbothrhizobiaandarbuscularmycorrhizalfungi AT quintocarmen autophagyrelatedkinaseisessentialforthesymbioticrelationshipbetweenphaseolusvulgarisandbothrhizobiaandarbuscularmycorrhizalfungi AT sanchezfederico autophagyrelatedkinaseisessentialforthesymbioticrelationshipbetweenphaseolusvulgarisandbothrhizobiaandarbuscularmycorrhizalfungi |
_version_ |
1756008243300139008 |
spelling |
oai:localhost:20.500.12123-109542021-12-21T10:53:01Z An Autophagy-Related Kinase Is Essential for the Symbiotic Relationship between Phaseolus vulgaris and Both Rhizobia and Arbuscular Mycorrhizal Fungi Estrada-Navarrete, Georgina Cruz-Mireles, Neftaly Lascano, Hernan Ramiro Alvarado-Affantranger, Xóchitl Hernández-Barrera, Alejandra Barraza, Aarón Olivares, Juan E. Arthikala, Manoj-Kumar Cárdenas, Luis Quinto, Carmen Sanchez, Federico Phaseolus Vulgaris Rhizobiaceae Arbuscular Mycorrhiza Kinases Micorrizas Arbusculares Quinasas Poroto Eukaryotes contain three types of lipid kinases that belong to the phosphatidylinositol 3-kinase (PI3K) family. In plants and Saccharomyces cerevisiae, only PI3K class III family members have been identified. These enzymes regulate the innate immune response, intracellular trafficking, autophagy, and senescence. Here, we report that RNAi-mediated downregulation of common bean (Phaseolus vulgaris) PI3K severely impaired symbiosis in composite P. vulgaris plants with endosymbionts such as Rhizobium tropici and Rhizophagus irregularis. Downregulation of Pv-PI3K was associated with a marked decrease in root hair growth and curling. Additionally, infection thread growth, root-nodule number, and symbiosome formation in root nodule cells were severely affected. Interestingly, root colonization by AM fungi and the formation of arbuscules were also abolished in PI3K loss-of-function plants. Furthermore, the transcript accumulation of genes encoding proteins known to interact with PI3K to form protein complexes involved in autophagy was drastically reduced in these transgenic roots. RNAi-mediated downregulation of one of these genes, Beclin1/Atg6, resulted in a similar phenotype as observed for transgenic roots in which Pv-PI3K had been downregulated. Our findings show that an autophagy-related process is crucial for the mutualistic interactions of P. vulgaris with beneficial microorganisms Instituto de Fisiología y Recursos Genéticos Vegetales Fil: Estrada-Navarrete, Georgina. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; México Fil: Cruz-Mireles, Neftaly. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; México Fil: Lascano, Hernán Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina Fil: Lascano, Hernán Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales. Argentina Fil: Alvarado-Affantranger, Xóchitl. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Laboratorio Nacional de Microscopía Avanzada; México Fil: Hernández-Barrera, Alejandra. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; México Fil: Barraza, Aarón. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; México Fil: Olivares, Juan E. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; México Fil: Arthikala, Manoj-Kumar. Universidad Nacional Autónoma de México. Escuela Nacional de Estudios Superiores-Unidad León; México Fil: Cárdenas, Luis. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; México Fil: Quinto, Carmen. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; México Fil: Sanchez, Federico. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; México 2021-12-21T10:46:52Z 2021-12-21T10:46:52Z 2016-09 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/10954 https://academic.oup.com/plcell/article/28/9/2326/6098387 1040-4651 1532-298X (online) https://doi.org/10.1105/tpc.15.01012 eng info:eu-repo/semantics/openAccess application/pdf American Society of Plant Biologists The Plant Cell 28 (9) : 2326-2341(September 2016) |