Type III-B CRISPR-Cas cascade of proteolytic cleavages

The generation of cyclic oligoadenylates and subsequent allosteric activation of proteins that carry sensory domains is a distinctive feature of type III CRISPR-Cas systems. In this work, we characterize a set of associated genes of a type III-B system from Haliangium ochraceum that contains two caspase-like proteases, SAVED-CHAT and PCaspase (prokaryotic caspase), co-opted from a cyclic oligonucleotide–based antiphage signaling system (CBASS). Cyclic tri–adenosine monophosphate (AMP)–induced oligomerization of SAVED-CHAT activates proteolytic activity of the CHAT domains, which specifically cleave and activate PCaspase. Subsequently, activated PCaspase cleaves a multitude of proteins, which results in a strong interference phenotype in vivo in Escherichia coli. Taken together, our findings reveal how a CRISPR-Cas–based detection of a target RNA triggers a cascade of caspase-associated proteolytic activities.

Bibliographic Details

Main Authors:	Steens, Jurre A., Bravo, Jack P.K., Salazar, Carl Raymund P., Yildiz, Caglar, Amieiro, Afonso M., Köstlbacher, Stephan, Prinsen, Stijn H.P., Andres, Ane S., Patinios, Constantinos, Bardis, Andreas, Barendregt, Arjan, Scheltema, Richard A., Ettema, Thijs J.G., van der Oost, John, Taylor, David W., Staals, Raymond H.J.
Format:	Article/Letter to editor biblioteca
Language:	English
Subjects:	Life Science,
Online Access:	https://research.wur.nl/en/publications/type-iii-b-crispr-cas-cascade-of-proteolytic-cleavages
Tags:	Add Tag No Tags, Be the first to tag this record!