Incorporation of (15)N-TNT transformation products into humifying plant organic matter as revealed by one- and two-dimensional solid state NMR spectroscopy

Incorporation of (15)N-TNT transformation products into humifying plant organic matter as revealed by one- and two-dimensional solid state NMR spectroscopy

10 pages, 3 figures, 1 table, 26 references.

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Bibliographic Details
Main Author: Knicker, Heike
Other Authors: Federal Ministry of Education and Research (Germany)
Format: artículo biblioteca
Language:English
Published: Elsevier 2003-06-01
Subjects:TNT-immobilization, Formation of amides, Polyamide-type macromolecule, Remediation, Double cross polarization magic angle spinning 15N 13C NMR; 1, Isotopic labeling,
Online Access:http://hdl.handle.net/10261/75451
http://dx.doi.org/10.13039/501100002347
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spelling dig-irnas-es-10261-754512018-09-12T11:56:53Z Incorporation of (15)N-TNT transformation products into humifying plant organic matter as revealed by one- and two-dimensional solid state NMR spectroscopy Knicker, Heike Federal Ministry of Education and Research (Germany) TNT-immobilization Formation of amides Polyamide-type macromolecule Remediation Double cross polarization magic angle spinning 15N 13C NMR; 1 Isotopic labeling 10 pages, 3 figures, 1 table, 26 references. Solid-state double cross polarization magic angle spinning (DCPMAS) 15N 13C nuclear magnetic resonance (NMR) spectroscopy was applied to study the incorporation of TNT transformation products into humifying plant organic matter. For this approach, 13C-enriched plant material (Lolium perenne) was mixed with quartz sand and aerobically incubated for 11 months after addition of 15N3-2,4,6-trinitrotoluene (TNT). After successive extraction of the incubate with water, methanol and ethyl acetate, approximately 60% of the 15N added as 15N3-TNT (15Nadd) remained in the solid organic residue (SOR-fraction). The acid insoluble fraction (AI) obtained after NaOH and HCl extractions contained approximately 20% of 15Nadd. For both fractions, 15N NMR spectroscopy revealed an almost complete reduction of the TNT after 11 months of aerobic incubation. Most of the reduced nitrogen groups underwent further condensation. The corresponding DCPMAS NMR spectra allowed the identification of amides that are further substituted by alkyl groups that resist even acid hydrolysis. This assigns them to relatively stable compounds rather than to newly synthesized microbial peptides. The results of this study suggest further that the covalent binding of TNT transformation products to plant derived organic matter is mediated by alkylation and acetylation reactions, rather than by 1,4 addition of TNT-derived nitrogenous groups to quinones of the humic material. This work was supported by the Federal Ministry of Education, Science, Research and Technology (BMBF, grant no.1471155). Peer reviewed 2013-05-02T11:51:25Z 2013-05-02T11:51:25Z 2003-06-01 artículo http://purl.org/coar/resource_type/c_6501 Science of the Total Environment 308 (1-3): 211-220 (2003) 0048-9697 http://hdl.handle.net/10261/75451 1879-1026 http://dx.doi.org/10.13039/501100002347 en open Elsevier
institution IRNAS ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-irnas-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del IRNAS España
language English
topic TNT-immobilization
Formation of amides
Polyamide-type macromolecule
Remediation
Double cross polarization magic angle spinning 15N 13C NMR; 1
Isotopic labeling
TNT-immobilization
Formation of amides
Polyamide-type macromolecule
Remediation
Double cross polarization magic angle spinning 15N 13C NMR; 1
Isotopic labeling
spellingShingle TNT-immobilization
Formation of amides
Polyamide-type macromolecule
Remediation
Double cross polarization magic angle spinning 15N 13C NMR; 1
Isotopic labeling
TNT-immobilization
Formation of amides
Polyamide-type macromolecule
Remediation
Double cross polarization magic angle spinning 15N 13C NMR; 1
Isotopic labeling
Knicker, Heike
Incorporation of (15)N-TNT transformation products into humifying plant organic matter as revealed by one- and two-dimensional solid state NMR spectroscopy
description 10 pages, 3 figures, 1 table, 26 references.
author2 Federal Ministry of Education and Research (Germany)
author_facet Federal Ministry of Education and Research (Germany)
Knicker, Heike
format artículo
topic_facet TNT-immobilization
Formation of amides
Polyamide-type macromolecule
Remediation
Double cross polarization magic angle spinning 15N 13C NMR; 1
Isotopic labeling
author Knicker, Heike
author_sort Knicker, Heike
title Incorporation of (15)N-TNT transformation products into humifying plant organic matter as revealed by one- and two-dimensional solid state NMR spectroscopy
title_short Incorporation of (15)N-TNT transformation products into humifying plant organic matter as revealed by one- and two-dimensional solid state NMR spectroscopy
title_full Incorporation of (15)N-TNT transformation products into humifying plant organic matter as revealed by one- and two-dimensional solid state NMR spectroscopy
title_fullStr Incorporation of (15)N-TNT transformation products into humifying plant organic matter as revealed by one- and two-dimensional solid state NMR spectroscopy
title_full_unstemmed Incorporation of (15)N-TNT transformation products into humifying plant organic matter as revealed by one- and two-dimensional solid state NMR spectroscopy
title_sort incorporation of (15)n-tnt transformation products into humifying plant organic matter as revealed by one- and two-dimensional solid state nmr spectroscopy
publisher Elsevier
publishDate 2003-06-01
url http://hdl.handle.net/10261/75451
http://dx.doi.org/10.13039/501100002347
work_keys_str_mv AT knickerheike incorporationof15ntnttransformationproductsintohumifyingplantorganicmatterasrevealedbyoneandtwodimensionalsolidstatenmrspectroscopy
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