Archaeal communities in two disparate deteriorated ancient wall paintings: Detection, identification and temporal monitoring by denaturing gradient gel electrophoresis
In the present study we describe the detection of Archaea in two different deteriorated ancient wall paintings, located in Austria and Spain, under different humidity/salinity regimes and climates. Archaeal communities were analyzed by combination of denaturing gradient gel electrophoresis (DGGE) of PCR-amplified DNA encoding 16S rRNA and the construction of clone libraries. DGGE analysis was used for temporal monitoring of the archaeal communities as well as for screening of the clone libraries. Selected clones were compared with band patterns obtained from the original samples and sequenced. Sequence analyses documented the existence of species belonging to well-characterized halophilic and alkaliphilic Archaea.
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Blackwell Publishing
2001
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| Online Access: | http://hdl.handle.net/10261/58260 |
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dig-irnas-es-10261-582602018-09-13T09:00:05Z Archaeal communities in two disparate deteriorated ancient wall paintings: Detection, identification and temporal monitoring by denaturing gradient gel electrophoresis Piñar, Guadalupe Sáiz-Jiménez, Cesáreo Schabereiter-Gurtner, Claudia Blanco-Varela, María Teresa Lubitz, Werner Rölleke, Sabine In the present study we describe the detection of Archaea in two different deteriorated ancient wall paintings, located in Austria and Spain, under different humidity/salinity regimes and climates. Archaeal communities were analyzed by combination of denaturing gradient gel electrophoresis (DGGE) of PCR-amplified DNA encoding 16S rRNA and the construction of clone libraries. DGGE analysis was used for temporal monitoring of the archaeal communities as well as for screening of the clone libraries. Selected clones were compared with band patterns obtained from the original samples and sequenced. Sequence analyses documented the existence of species belonging to well-characterized halophilic and alkaliphilic Archaea. This work is part of the European Union funded project `Novel molecular tools for the analysis of unknown micro- bial communities of mural paintings and their implemen- tation into the conservation/restoration practice' (ENV4- CT98-0705). This work was supported by the European Community `Marie Curie' Training Grant (Contract BIO4-CT98-5057). Peer Reviewed 2012-10-17T11:41:41Z 2012-10-17T11:41:41Z 2001 2012-10-17T11:41:41Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1016/S0168-6496(01)00144-1 issn: 0168-6496 e-issn: 1574-6941 FEMS Micriobiology Ecology 37: 45- 54 (2001) http://hdl.handle.net/10261/58260 10.1016/S0168-6496(01)00144-1 en none Blackwell Publishing |
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In the present study we describe the detection of Archaea in two different deteriorated ancient wall paintings, located in Austria and Spain, under different humidity/salinity regimes and climates. Archaeal communities were analyzed by combination of denaturing gradient gel electrophoresis (DGGE) of PCR-amplified DNA encoding 16S rRNA and the construction of clone libraries. DGGE analysis was used for temporal monitoring of the archaeal communities as well as for screening of the clone libraries. Selected clones were compared with band patterns obtained from the original samples and sequenced. Sequence analyses documented the existence of species belonging to well-characterized halophilic and alkaliphilic Archaea. |
| format |
artículo |
| author |
Piñar, Guadalupe Sáiz-Jiménez, Cesáreo Schabereiter-Gurtner, Claudia Blanco-Varela, María Teresa Lubitz, Werner Rölleke, Sabine |
| spellingShingle |
Piñar, Guadalupe Sáiz-Jiménez, Cesáreo Schabereiter-Gurtner, Claudia Blanco-Varela, María Teresa Lubitz, Werner Rölleke, Sabine Archaeal communities in two disparate deteriorated ancient wall paintings: Detection, identification and temporal monitoring by denaturing gradient gel electrophoresis |
| author_facet |
Piñar, Guadalupe Sáiz-Jiménez, Cesáreo Schabereiter-Gurtner, Claudia Blanco-Varela, María Teresa Lubitz, Werner Rölleke, Sabine |
| author_sort |
Piñar, Guadalupe |
| title |
Archaeal communities in two disparate deteriorated ancient wall paintings: Detection, identification and temporal monitoring by denaturing gradient gel electrophoresis |
| title_short |
Archaeal communities in two disparate deteriorated ancient wall paintings: Detection, identification and temporal monitoring by denaturing gradient gel electrophoresis |
| title_full |
Archaeal communities in two disparate deteriorated ancient wall paintings: Detection, identification and temporal monitoring by denaturing gradient gel electrophoresis |
| title_fullStr |
Archaeal communities in two disparate deteriorated ancient wall paintings: Detection, identification and temporal monitoring by denaturing gradient gel electrophoresis |
| title_full_unstemmed |
Archaeal communities in two disparate deteriorated ancient wall paintings: Detection, identification and temporal monitoring by denaturing gradient gel electrophoresis |
| title_sort |
archaeal communities in two disparate deteriorated ancient wall paintings: detection, identification and temporal monitoring by denaturing gradient gel electrophoresis |
| publisher |
Blackwell Publishing |
| publishDate |
2001 |
| url |
http://hdl.handle.net/10261/58260 |
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