Early attack and subsequent changes produced in an industrial lignin by a fungal laccase and a laccase-mediator system: an analytical approach
An industrial kraft pine lignin (Indulin AT, KL) was characterized and treated in both aqueous-buffered media and dioxane to water, either with a partially purified laccase from Fusarium proliferatum or with the laccase plus 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic-acid (ABTS) as mediator. The changes in the lignin after different incubation periods were analyzed through the application of high performance liquid chromatography (HPLC), UV-visible (Vis) spectroscopy and pyrolysis-gas chromatography/mass spectrometry (Py-GC/MS). At the onset of incubation, laccase-treated samples showed a slight polymerization and strong modifications in UV-Vis spectra. Through Py-GC/MS, a decrease in phenolic and methoxy-bearing pyrolysis products was observed, in contrast to an increase in the more oxidized products. After longer incubation periods (48 h) a substantial polymerization was detected by HPLC, along with a decrease in the guaiacyl (G) units. In contrast, the analysis by HPLC of the samples recovered from the laccase-ABTS system (LMS) showed an intense depolymerization, accompanied by a sizeable loss in G units and a decrease in the methyl and ethyl side-chain phenolic compounds. These results provide conclusive evidence of a rapid initial attack of the industrial lignin by laccase and notable modifications in the KL after longer incubation periods with laccase or LMS. © 2006 Springer-Verlag.
Main Authors: | González Arzola, K., Polvillo, Oliva, Arias Fernández, Mª E., Perestelo, F., Carnicero, A., González-Vila, Francisco Javier, Falcón, M. A. |
---|---|
Other Authors: | Ministerio de Ciencia y Tecnología (España) |
Format: | artículo biblioteca |
Language: | English |
Published: |
Springer
2006
|
Online Access: | http://hdl.handle.net/10261/57946 http://dx.doi.org/10.13039/501100006280 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Laccase-Mediator Pretreatment of Wheat Straw Degrades Lignin and Improves Saccharification
by: Rencoret, Jorge, et al. -
Flax pulp bleaching and residual lignin modification by laccase-mediator systems
by: Camarero, Susana, et al.
Published: (2002) -
Pretreatment of wheat straw with laccase mediator system degrades lignin and improves saccharification
by: Rencoret, Jorge, et al.
Published: (2016-06-19) -
Fungal laccases: induction and production
by: Bertrand,B., et al.
Published: (2013) -
Lignin changes after steam explosion and laccase-mediator treatment of eucalyptus wood chips
by: Martin-Sampedro, R., et al.
Published: (2011)