HSQC-NMR analysis of lignin in woody (Eucalyptus globulus and Picea abies) and non-woody (Agave sisalana) ball-milled plant materials at the gel state
8 páginas, 2 tablas, 2 figuras.
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Other Authors: | |
| Format: | artículo biblioteca |
| Language: | English |
| Published: |
Walter de Gruyter
|
| Subjects: | 2D NMR, Acylation, Eucalypt, HSQC, Lignin structure, MWL, Sisal, Spruce, Wood, |
| Online Access: | http://hdl.handle.net/10261/34834 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100004837 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
dig-irnas-es-10261-34834 |
|---|---|
| record_format |
koha |
| spelling |
dig-irnas-es-10261-348342020-11-13T13:29:41Z HSQC-NMR analysis of lignin in woody (Eucalyptus globulus and Picea abies) and non-woody (Agave sisalana) ball-milled plant materials at the gel state Rencoret, Jorge Marques, Gisela Gutiérrez Suárez, Ana Nieto, Lidia Santos, José Ignacio Jiménez-Barbero, Jesús Martínez, Ángel T. Río Andrade, José Carlos del Ministerio de Ciencia e Innovación (España) Consejo Superior de Investigaciones Científicas (España) European Commission Consejo Superior de Investigaciones Científicas (España) Ministerio de Educación y Ciencia (España) 2D NMR Acylation Eucalypt HSQC Lignin structure MWL Sisal Spruce Wood 8 páginas, 2 tablas, 2 figuras. In situ analysis of lignin by 2D NMR of whole plant material was carried out by swelling finely ball-milled samples in deuterated dimethylsulfoxide (DMSO-d6) and sonicated so that a gel was formed in the NMR analysis tube. Solution HSQC NMR spectra of different plant materials representative for hardwood (Eucalyptus globulus), softwood (Picea abies), and non-woody plants (Agave sisalana) are presented here. The spectra show signals corresponding to those of the main plant constituents such as lignin and polysaccharides. The lignin signals were assigned by comparing the HSQC spectra of the whole plant materials with the HSQC spectra of their respective milled-wood lignins (MWL). In general terms, the major lignin structural features, such as the relative abundances of the main lignin substructures, the syringyl/guaiacyl ratios and the extent of γ-acetylation of the lignin side-chain observed in the HSQC spectra of the whole plant materials, matched those obtained from the HSQC spectra of the isolated MWL. Therefore, this technique, which needs only minor amounts of lignocellulosic material and minimal sample preparation, can be useful for the rapid screening of plant lignins without the need for tedious and time-consuming lignin isolation procedures. This study has been supported by the Spanish Ministry of Science and Innovation (projects AGL2005-01748, BIO2005-03569 and BIO2008-01533), CSIC (project 2006-4-0I-039) and European Commission (BIORENEW project NMP2-CT-2006-026456). J.R. thanks the CSIC for a I3P fellowship and GM thanks the Spanish MEC for a FPI fellowship. Peer reviewed 2009 artículo http://purl.org/coar/resource_type/c_6501 Holzforschung 63: 691-698 (2009) 0018-3830 http://hdl.handle.net/10261/34834 10.1515/HF.2009.070 1437-434X http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100004837 en http://dx.doi.org/10.1515/HF.2009.070 open Walter de Gruyter |
| institution |
IRNAS ES |
| collection |
DSpace |
| country |
España |
| countrycode |
ES |
| component |
Bibliográfico |
| access |
En linea |
| databasecode |
dig-irnas-es |
| tag |
biblioteca |
| region |
Europa del Sur |
| libraryname |
Biblioteca del IRNAS España |
| language |
English |
| topic |
2D NMR Acylation Eucalypt HSQC Lignin structure MWL Sisal Spruce Wood 2D NMR Acylation Eucalypt HSQC Lignin structure MWL Sisal Spruce Wood |
| spellingShingle |
2D NMR Acylation Eucalypt HSQC Lignin structure MWL Sisal Spruce Wood 2D NMR Acylation Eucalypt HSQC Lignin structure MWL Sisal Spruce Wood Rencoret, Jorge Marques, Gisela Gutiérrez Suárez, Ana Nieto, Lidia Santos, José Ignacio Jiménez-Barbero, Jesús Martínez, Ángel T. Río Andrade, José Carlos del HSQC-NMR analysis of lignin in woody (Eucalyptus globulus and Picea abies) and non-woody (Agave sisalana) ball-milled plant materials at the gel state |
| description |
8 páginas, 2 tablas, 2 figuras. |
| author2 |
Ministerio de Ciencia e Innovación (España) |
| author_facet |
Ministerio de Ciencia e Innovación (España) Rencoret, Jorge Marques, Gisela Gutiérrez Suárez, Ana Nieto, Lidia Santos, José Ignacio Jiménez-Barbero, Jesús Martínez, Ángel T. Río Andrade, José Carlos del |
| format |
artículo |
| topic_facet |
2D NMR Acylation Eucalypt HSQC Lignin structure MWL Sisal Spruce Wood |
| author |
Rencoret, Jorge Marques, Gisela Gutiérrez Suárez, Ana Nieto, Lidia Santos, José Ignacio Jiménez-Barbero, Jesús Martínez, Ángel T. Río Andrade, José Carlos del |
| author_sort |
Rencoret, Jorge |
| title |
HSQC-NMR analysis of lignin in woody (Eucalyptus globulus and Picea abies) and non-woody (Agave sisalana) ball-milled plant materials at the gel state |
| title_short |
HSQC-NMR analysis of lignin in woody (Eucalyptus globulus and Picea abies) and non-woody (Agave sisalana) ball-milled plant materials at the gel state |
| title_full |
HSQC-NMR analysis of lignin in woody (Eucalyptus globulus and Picea abies) and non-woody (Agave sisalana) ball-milled plant materials at the gel state |
| title_fullStr |
HSQC-NMR analysis of lignin in woody (Eucalyptus globulus and Picea abies) and non-woody (Agave sisalana) ball-milled plant materials at the gel state |
| title_full_unstemmed |
HSQC-NMR analysis of lignin in woody (Eucalyptus globulus and Picea abies) and non-woody (Agave sisalana) ball-milled plant materials at the gel state |
| title_sort |
hsqc-nmr analysis of lignin in woody (eucalyptus globulus and picea abies) and non-woody (agave sisalana) ball-milled plant materials at the gel state |
| publisher |
Walter de Gruyter |
| url |
http://hdl.handle.net/10261/34834 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100004837 |
| work_keys_str_mv |
AT rencoretjorge hsqcnmranalysisoflignininwoodyeucalyptusglobulusandpiceaabiesandnonwoodyagavesisalanaballmilledplantmaterialsatthegelstate AT marquesgisela hsqcnmranalysisoflignininwoodyeucalyptusglobulusandpiceaabiesandnonwoodyagavesisalanaballmilledplantmaterialsatthegelstate AT gutierrezsuarezana hsqcnmranalysisoflignininwoodyeucalyptusglobulusandpiceaabiesandnonwoodyagavesisalanaballmilledplantmaterialsatthegelstate AT nietolidia hsqcnmranalysisoflignininwoodyeucalyptusglobulusandpiceaabiesandnonwoodyagavesisalanaballmilledplantmaterialsatthegelstate AT santosjoseignacio hsqcnmranalysisoflignininwoodyeucalyptusglobulusandpiceaabiesandnonwoodyagavesisalanaballmilledplantmaterialsatthegelstate AT jimenezbarberojesus hsqcnmranalysisoflignininwoodyeucalyptusglobulusandpiceaabiesandnonwoodyagavesisalanaballmilledplantmaterialsatthegelstate AT martinezangelt hsqcnmranalysisoflignininwoodyeucalyptusglobulusandpiceaabiesandnonwoodyagavesisalanaballmilledplantmaterialsatthegelstate AT rioandradejosecarlosdel hsqcnmranalysisoflignininwoodyeucalyptusglobulusandpiceaabiesandnonwoodyagavesisalanaballmilledplantmaterialsatthegelstate |
| _version_ |
1777664174707441664 |