A novel thiol-reductase activity of Arabidopsis YUC6 confers drought tolerance independently of auxin biosynthesis

A novel thiol-reductase activity of Arabidopsis YUC6 confers drought tolerance independently of auxin biosynthesis

13 páginas.-- 7 figuras.-- 69 referencias.-- Supplementary Information: Supplementary Figures 1-7 and Supplementary Tables 1-2

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Main Authors: Cha, Joon-Yung, Pardo, José M., Yun, Dae-Jin
Format: artículo biblioteca
Language:English
Published: Nature Publishing Group 2015-08-28
Subjects:Biological sciences, Biochemistry, Plant sciences,
Online Access:http://hdl.handle.net/10261/121595
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spelling dig-irnas-es-10261-1215952021-12-28T16:08:35Z A novel thiol-reductase activity of Arabidopsis YUC6 confers drought tolerance independently of auxin biosynthesis Cha, Joon-Yung Pardo, José M. Yun, Dae-Jin Biological sciences Biochemistry Plant sciences 13 páginas.-- 7 figuras.-- 69 referencias.-- Supplementary Information: Supplementary Figures 1-7 and Supplementary Tables 1-2 YUCCA (YUC) proteins constitute a family of flavin monooxygenases (FMOs), with an important role in auxin (IAA) biosynthesis. Here we report that Arabidopsis plants overexpressing YUC6 display enhanced IAA-related phenotypes and exhibit improved drought stress tolerance, low rate of water loss and controlled ROS accumulation under drought and oxidative stresses. Co-overexpression of an IAA-conjugating enzyme reduces IAA levels but drought stress tolerance is unaffected, indicating that the stress-related phenotype is not based on IAA overproduction. YUC6 contains a previously unrecognized FAD- and NADPH-dependent thiol-reductase activity (TR) that overlaps with the FMO domain involved in IAA biosynthesis. Mutation of a conserved cysteine residue (Cys-85) preserves FMO but suppresses TR activity and stress tolerance, whereas mutating the FAD- and NADPH-binding sites, that are common to TR and FMO domains, abolishes all outputs. We provide a paradigm for a single protein playing a dual role, regulating plant development and conveying stress defence responses. We thank Dr Yunde Zhao for generously sharing the quadruple mutant yuc1,2,4,6 seeds and Dr Verena Kriechbaumer for kindly supporting comments for in vitro YUC assay. This work was supported by grants from the National Research Foundation of Korea (NRF) funded by the Korean Government (MSIP; 2013R1A2A1A01005170) and Next-Generation BioGreen21 Program (SSAC, PJ01106901), Rural Development Administration, Republic of Korea. J.-Y.C. and M.R.K. were supported by a scholarship from the BK21Plus Program, the Ministry of Education, Korea. M.N. and T.A. were supported by grants from Core Research for Evolutional Science and Technology (CREST) of Japan Science and Technology Agency (JST), Japan Peer reviewed 2015-09-03T05:14:19Z 2015-09-03T05:14:19Z 2015-08-28 artículo http://purl.org/coar/resource_type/c_6501 Nature Communications 6: 8041 (2015) http://hdl.handle.net/10261/121595 10.1038/ncomms9041 2041-1723 26314500 en Publisher's version http://dx.doi.org/10.1038/ncomms9041 Sí open Nature Publishing Group
institution IRNAS ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-irnas-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del IRNAS España
language English
topic Biological sciences
Biochemistry
Plant sciences
Biological sciences
Biochemistry
Plant sciences
spellingShingle Biological sciences
Biochemistry
Plant sciences
Biological sciences
Biochemistry
Plant sciences
Cha, Joon-Yung
Pardo, José M.
Yun, Dae-Jin
A novel thiol-reductase activity of Arabidopsis YUC6 confers drought tolerance independently of auxin biosynthesis
description 13 páginas.-- 7 figuras.-- 69 referencias.-- Supplementary Information: Supplementary Figures 1-7 and Supplementary Tables 1-2
format artículo
topic_facet Biological sciences
Biochemistry
Plant sciences
author Cha, Joon-Yung
Pardo, José M.
Yun, Dae-Jin
author_facet Cha, Joon-Yung
Pardo, José M.
Yun, Dae-Jin
author_sort Cha, Joon-Yung
title A novel thiol-reductase activity of Arabidopsis YUC6 confers drought tolerance independently of auxin biosynthesis
title_short A novel thiol-reductase activity of Arabidopsis YUC6 confers drought tolerance independently of auxin biosynthesis
title_full A novel thiol-reductase activity of Arabidopsis YUC6 confers drought tolerance independently of auxin biosynthesis
title_fullStr A novel thiol-reductase activity of Arabidopsis YUC6 confers drought tolerance independently of auxin biosynthesis
title_full_unstemmed A novel thiol-reductase activity of Arabidopsis YUC6 confers drought tolerance independently of auxin biosynthesis
title_sort novel thiol-reductase activity of arabidopsis yuc6 confers drought tolerance independently of auxin biosynthesis
publisher Nature Publishing Group
publishDate 2015-08-28
url http://hdl.handle.net/10261/121595
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