Synthesis and effects on cell viability of flavonols and 3-methyl ether derivatives on human leukemia cells
Flavonoids are polyphenolic compounds which display an array of biological activities and are considered potential antitumor agents. Here we evaluated the antiproliferative activity of selected synthetic flavonoids against human leukemia cell lines. We found that 4′-bromoflavonol (flavonol 3) was the most potent. This compound inhibited proliferation in a concentration-dependent manner, induced apoptosis and blocked cell cycle progression at the S phase. Cell death was found to be associated with the cleavage and activation of multiple caspases, the activation of the mitogen-activated protein kinase pathway and the up-regulation of two death receptors (death receptor 4 and death receptor 5) for tumor necrosis factor-related apoptosis-inducing ligand. Moreover, combined treatments using 4′-bromoflavonol and TRAIL led to an increased cytotoxicity compared to single treatments. These results provide a basis for further exploring the potential applications of this combination for the treatment of cancer.
Main Authors: | , , , , , , |
---|---|
Other Authors: | |
Format: | artículo biblioteca |
Published: |
Elsevier
2014-09-12
|
Subjects: | Death receptors, Flavonoids, Apoptosis, Cytotoxicity, Cell cycle, Caspases, |
Online Access: | http://hdl.handle.net/10261/179981 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100004837 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-ipna-es-10261-179981 |
---|---|
record_format |
koha |
spelling |
dig-ipna-es-10261-1799812020-12-09T17:42:42Z Synthesis and effects on cell viability of flavonols and 3-methyl ether derivatives on human leukemia cells Burmistrova, Olga Marrero, María Teresa Estévez, Sara Welsch, Isabel Brouard, Ignacio Quintana, José Estévez, Francisco Ministerio de Ciencia e Innovación (España) European Commission Consejo Superior de Investigaciones Científicas (España) Fundación Universitaria de Las Palmas Death receptors Flavonoids Apoptosis Cytotoxicity Cell cycle Caspases Flavonoids are polyphenolic compounds which display an array of biological activities and are considered potential antitumor agents. Here we evaluated the antiproliferative activity of selected synthetic flavonoids against human leukemia cell lines. We found that 4′-bromoflavonol (flavonol 3) was the most potent. This compound inhibited proliferation in a concentration-dependent manner, induced apoptosis and blocked cell cycle progression at the S phase. Cell death was found to be associated with the cleavage and activation of multiple caspases, the activation of the mitogen-activated protein kinase pathway and the up-regulation of two death receptors (death receptor 4 and death receptor 5) for tumor necrosis factor-related apoptosis-inducing ligand. Moreover, combined treatments using 4′-bromoflavonol and TRAIL led to an increased cytotoxicity compared to single treatments. These results provide a basis for further exploring the potential applications of this combination for the treatment of cancer. This work was supported by the Spanish Ministry of Science and Innovation and the European Regional Development Fund (SAF2010-21380) and CSIC (Proyecto Intramural de Incorporación – 2007022). O. B. was supported by a research studentship from the Fundación Canaria Universitaria de Las Palmas. Peer Reviewed 2019-04-12T07:40:19Z 2019-04-12T07:40:19Z 2014-09-12 2019-04-12T07:40:19Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1016/j.ejmech.2014.07.010 issn: 0223-5234 e-issn: 1768-3254 European Journal of Medicinal Chemistry 84: 30-41 (2014) http://hdl.handle.net/10261/179981 10.1016/j.ejmech.2014.07.010 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100004837 https://doi.org/10.1016/j.ejmech.2014.07.010 Sí none Elsevier |
institution |
IPNA ES |
collection |
DSpace |
country |
España |
countrycode |
ES |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-ipna-es |
tag |
biblioteca |
region |
Europa del Sur |
libraryname |
Biblioteca del IPNA España |
topic |
Death receptors Flavonoids Apoptosis Cytotoxicity Cell cycle Caspases Death receptors Flavonoids Apoptosis Cytotoxicity Cell cycle Caspases |
spellingShingle |
Death receptors Flavonoids Apoptosis Cytotoxicity Cell cycle Caspases Death receptors Flavonoids Apoptosis Cytotoxicity Cell cycle Caspases Burmistrova, Olga Marrero, María Teresa Estévez, Sara Welsch, Isabel Brouard, Ignacio Quintana, José Estévez, Francisco Synthesis and effects on cell viability of flavonols and 3-methyl ether derivatives on human leukemia cells |
description |
Flavonoids are polyphenolic compounds which display an array of biological activities and are considered potential antitumor agents. Here we evaluated the antiproliferative activity of selected synthetic flavonoids against human leukemia cell lines. We found that 4′-bromoflavonol (flavonol 3) was the most potent. This compound inhibited proliferation in a concentration-dependent manner, induced apoptosis and blocked cell cycle progression at the S phase. Cell death was found to be associated with the cleavage and activation of multiple caspases, the activation of the mitogen-activated protein kinase pathway and the up-regulation of two death receptors (death receptor 4 and death receptor 5) for tumor necrosis factor-related apoptosis-inducing ligand. Moreover, combined treatments using 4′-bromoflavonol and TRAIL led to an increased cytotoxicity compared to single treatments. These results provide a basis for further exploring the potential applications of this combination for the treatment of cancer. |
author2 |
Ministerio de Ciencia e Innovación (España) |
author_facet |
Ministerio de Ciencia e Innovación (España) Burmistrova, Olga Marrero, María Teresa Estévez, Sara Welsch, Isabel Brouard, Ignacio Quintana, José Estévez, Francisco |
format |
artículo |
topic_facet |
Death receptors Flavonoids Apoptosis Cytotoxicity Cell cycle Caspases |
author |
Burmistrova, Olga Marrero, María Teresa Estévez, Sara Welsch, Isabel Brouard, Ignacio Quintana, José Estévez, Francisco |
author_sort |
Burmistrova, Olga |
title |
Synthesis and effects on cell viability of flavonols and 3-methyl ether derivatives on human leukemia cells |
title_short |
Synthesis and effects on cell viability of flavonols and 3-methyl ether derivatives on human leukemia cells |
title_full |
Synthesis and effects on cell viability of flavonols and 3-methyl ether derivatives on human leukemia cells |
title_fullStr |
Synthesis and effects on cell viability of flavonols and 3-methyl ether derivatives on human leukemia cells |
title_full_unstemmed |
Synthesis and effects on cell viability of flavonols and 3-methyl ether derivatives on human leukemia cells |
title_sort |
synthesis and effects on cell viability of flavonols and 3-methyl ether derivatives on human leukemia cells |
publisher |
Elsevier |
publishDate |
2014-09-12 |
url |
http://hdl.handle.net/10261/179981 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100004837 |
work_keys_str_mv |
AT burmistrovaolga synthesisandeffectsoncellviabilityofflavonolsand3methyletherderivativesonhumanleukemiacells AT marreromariateresa synthesisandeffectsoncellviabilityofflavonolsand3methyletherderivativesonhumanleukemiacells AT estevezsara synthesisandeffectsoncellviabilityofflavonolsand3methyletherderivativesonhumanleukemiacells AT welschisabel synthesisandeffectsoncellviabilityofflavonolsand3methyletherderivativesonhumanleukemiacells AT brouardignacio synthesisandeffectsoncellviabilityofflavonolsand3methyletherderivativesonhumanleukemiacells AT quintanajose synthesisandeffectsoncellviabilityofflavonolsand3methyletherderivativesonhumanleukemiacells AT estevezfrancisco synthesisandeffectsoncellviabilityofflavonolsand3methyletherderivativesonhumanleukemiacells |
_version_ |
1777669757595549696 |