Detection and occurrence of melon yellow spot virus in Ecuador: an emerging threat to cucurbit production in the region
More than fifty viruses have been reported in cucurbit crops worldwide. In Ecuador, approximately 3,000 ha of watermelon, melon and cucumbers are cultivated annually, but there have been few studies to identify viruses responsible for epidemics. During this study, sequencing of double-stranded RNA (dsRNA) extracted from watermelon and melon leaves showing virus-like symptoms revealed the presence of Melon yellow spot virus (MYSV, genus Tospovirus) and the partially described Cucumis melo endornavirus (CmEV). Specific primers, designed to detect each virus, showed that MYSV was present in 40, 64 and 67 % of symptomatic watermelon, cucumber and melon samples, respectively. For CmEV, 95 % of both symptomatic and asymptomatic melon plants tested positive. However, the virus was not detected in watermelon or cucumber. Sequence comparisons showed nucleotide identities of 97 % and 94 % for the polymerase and the nucleocapsid protein, respectively, between the Ecuadorean MYSV and the one reported from Japan. To the best of our knowledge, this is the first report of MYSV and CmEV in Ecuador and the Americas.
Main Authors: | , , , , , , , |
---|---|
Format: | Journal articles biblioteca |
Language: | English |
Published: |
2014-10
|
Subjects: | MELÓN, CUCUMIS MELO, SANDÍA, CITRULLUS LANATUS, PEPINO, CUCUMIS SATIVUS, CUCURBITÁCEAS, TOSPOVIRUS, ENDORNAVIRUS, |
Online Access: | http://repositorio.iniap.gob.ec/handle/41000/4836 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-iniap-41000-4836 |
---|---|
record_format |
koha |
spelling |
dig-iniap-41000-48362018-04-19T20:21:58Z Detection and occurrence of melon yellow spot virus in Ecuador: an emerging threat to cucurbit production in the region European Journal of Plant Pathology 140(2):193–197 Quito-Avila, D.F. Peralta, E.L. Martin, R.R. Ibarra, M.A. Alvarez, R.A. Mendoza, A. Insuasti, María Luisa Ochoa L., José MELÓN CUCUMIS MELO SANDÍA CITRULLUS LANATUS PEPINO CUCUMIS SATIVUS CUCURBITÁCEAS TOSPOVIRUS ENDORNAVIRUS More than fifty viruses have been reported in cucurbit crops worldwide. In Ecuador, approximately 3,000 ha of watermelon, melon and cucumbers are cultivated annually, but there have been few studies to identify viruses responsible for epidemics. During this study, sequencing of double-stranded RNA (dsRNA) extracted from watermelon and melon leaves showing virus-like symptoms revealed the presence of Melon yellow spot virus (MYSV, genus Tospovirus) and the partially described Cucumis melo endornavirus (CmEV). Specific primers, designed to detect each virus, showed that MYSV was present in 40, 64 and 67 % of symptomatic watermelon, cucumber and melon samples, respectively. For CmEV, 95 % of both symptomatic and asymptomatic melon plants tested positive. However, the virus was not detected in watermelon or cucumber. Sequence comparisons showed nucleotide identities of 97 % and 94 % for the polymerase and the nucleocapsid protein, respectively, between the Ecuadorean MYSV and the one reported from Japan. To the best of our knowledge, this is the first report of MYSV and CmEV in Ecuador and the Americas. 2018-02-22T19:06:14Z 2018-02-22T19:06:14Z 2014-10 Revista Artículo 1573-8469 *EC-INIAP-BEESC-MGC. Quito ( European Journal of Plant Pathology 140(2):193–197) http://repositorio.iniap.gob.ec/handle/41000/4836 en p. 193–197 application/pdf E. E. Santa Catalina |
institution |
INIAP |
collection |
DSpace |
country |
Ecuador |
countrycode |
EC |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-iniap |
tag |
biblioteca |
region |
America del Sur |
libraryname |
Biblioteca INIAP |
language |
English |
topic |
MELÓN CUCUMIS MELO SANDÍA CITRULLUS LANATUS PEPINO CUCUMIS SATIVUS CUCURBITÁCEAS TOSPOVIRUS ENDORNAVIRUS MELÓN CUCUMIS MELO SANDÍA CITRULLUS LANATUS PEPINO CUCUMIS SATIVUS CUCURBITÁCEAS TOSPOVIRUS ENDORNAVIRUS |
spellingShingle |
MELÓN CUCUMIS MELO SANDÍA CITRULLUS LANATUS PEPINO CUCUMIS SATIVUS CUCURBITÁCEAS TOSPOVIRUS ENDORNAVIRUS MELÓN CUCUMIS MELO SANDÍA CITRULLUS LANATUS PEPINO CUCUMIS SATIVUS CUCURBITÁCEAS TOSPOVIRUS ENDORNAVIRUS Quito-Avila, D.F. Peralta, E.L. Martin, R.R. Ibarra, M.A. Alvarez, R.A. Mendoza, A. Insuasti, María Luisa Ochoa L., José Detection and occurrence of melon yellow spot virus in Ecuador: an emerging threat to cucurbit production in the region |
description |
More than fifty viruses have been reported in cucurbit crops worldwide. In Ecuador, approximately 3,000 ha of watermelon, melon and cucumbers are cultivated annually, but there have been few studies to identify viruses responsible for epidemics. During this study, sequencing of double-stranded RNA (dsRNA) extracted from watermelon and melon leaves showing virus-like symptoms revealed the presence of Melon yellow spot virus (MYSV, genus Tospovirus) and the partially described Cucumis melo endornavirus (CmEV). Specific primers, designed to detect each virus, showed that MYSV was present in 40, 64 and 67 % of symptomatic watermelon, cucumber and melon samples, respectively. For CmEV, 95 % of both symptomatic and asymptomatic melon plants tested positive. However, the virus was not detected in watermelon or cucumber. Sequence comparisons showed nucleotide identities of 97 % and 94 % for the polymerase and the nucleocapsid protein, respectively, between the Ecuadorean MYSV and the one reported from Japan. To the best of our knowledge, this is the first report of MYSV and CmEV in Ecuador and the Americas. |
format |
Revista |
topic_facet |
MELÓN CUCUMIS MELO SANDÍA CITRULLUS LANATUS PEPINO CUCUMIS SATIVUS CUCURBITÁCEAS TOSPOVIRUS ENDORNAVIRUS |
author |
Quito-Avila, D.F. Peralta, E.L. Martin, R.R. Ibarra, M.A. Alvarez, R.A. Mendoza, A. Insuasti, María Luisa Ochoa L., José |
author_facet |
Quito-Avila, D.F. Peralta, E.L. Martin, R.R. Ibarra, M.A. Alvarez, R.A. Mendoza, A. Insuasti, María Luisa Ochoa L., José |
author_sort |
Quito-Avila, D.F. |
title |
Detection and occurrence of melon yellow spot virus in Ecuador: an emerging threat to cucurbit production in the region |
title_short |
Detection and occurrence of melon yellow spot virus in Ecuador: an emerging threat to cucurbit production in the region |
title_full |
Detection and occurrence of melon yellow spot virus in Ecuador: an emerging threat to cucurbit production in the region |
title_fullStr |
Detection and occurrence of melon yellow spot virus in Ecuador: an emerging threat to cucurbit production in the region |
title_full_unstemmed |
Detection and occurrence of melon yellow spot virus in Ecuador: an emerging threat to cucurbit production in the region |
title_sort |
detection and occurrence of melon yellow spot virus in ecuador: an emerging threat to cucurbit production in the region |
publishDate |
2014-10 |
url |
http://repositorio.iniap.gob.ec/handle/41000/4836 |
work_keys_str_mv |
AT quitoaviladf detectionandoccurrenceofmelonyellowspotvirusinecuadoranemergingthreattocucurbitproductionintheregion AT peraltael detectionandoccurrenceofmelonyellowspotvirusinecuadoranemergingthreattocucurbitproductionintheregion AT martinrr detectionandoccurrenceofmelonyellowspotvirusinecuadoranemergingthreattocucurbitproductionintheregion AT ibarrama detectionandoccurrenceofmelonyellowspotvirusinecuadoranemergingthreattocucurbitproductionintheregion AT alvarezra detectionandoccurrenceofmelonyellowspotvirusinecuadoranemergingthreattocucurbitproductionintheregion AT mendozaa detectionandoccurrenceofmelonyellowspotvirusinecuadoranemergingthreattocucurbitproductionintheregion AT insuastimarialuisa detectionandoccurrenceofmelonyellowspotvirusinecuadoranemergingthreattocucurbitproductionintheregion AT ochoaljose detectionandoccurrenceofmelonyellowspotvirusinecuadoranemergingthreattocucurbitproductionintheregion AT quitoaviladf europeanjournalofplantpathology1402193197 AT peraltael europeanjournalofplantpathology1402193197 AT martinrr europeanjournalofplantpathology1402193197 AT ibarrama europeanjournalofplantpathology1402193197 AT alvarezra europeanjournalofplantpathology1402193197 AT mendozaa europeanjournalofplantpathology1402193197 AT insuastimarialuisa europeanjournalofplantpathology1402193197 AT ochoaljose europeanjournalofplantpathology1402193197 |
_version_ |
1756059996635791361 |