Determination of phytate and lower inositol phosphates in Spanish legumes by HPLC methodology
The separation and quantitative determination of phytic acid (inositol hexaphosphate) and lower inositol phosphates (tri-, tetra- and penta-phosphates) was carried out using a HPLC method that included purification by anion-exchange chromatography, and analysis by ion-pair chromatography on a C18 reverse phase column. Samples of the most important legumes in the Mediterranean diet were analysed using this system. The different samples presented a variable content of phytic acid and different relative proportions of lower inositol phosphates. © 1995.
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| Main Authors: | , , , , |
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| Format: | journal article biblioteca |
| Language: | eng |
| Published: |
1995
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| Online Access: | http://hdl.handle.net/20.500.12792/4963 |
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