Deleterious effects of progestagen treatment in VEGF expression in corpora lutea of pregnant ewes
Contents The aim of the current study was to determine the possible effects of progestagen oestrous synchronization on vascular endothelial growth factor (VEGF) expression during sheep luteogenesis and the peri-implantation period and the relationship with luteal function. At days 9, 11, 13, 15, 17 and 21 of pregnancy, the ovaries from 30 progestagen treated and 30 ewes cycling after cloprostenol injection were evaluated by ultrasonography and, thereafter, collected and processed for immunohistochemical evaluation of VEGF; blood samples were drawn for evaluating plasma progesterone. The progestagen-treated group showed smaller corpora lutea than cloprostenol-treated and lower progesterone secretion. The expression of VEGF in the luteal cells increased with time in the cloprostenol group, but not in the progestagen-treated group, which even showed a decrease between days 11 and 13. In progestagen-treated sheep, VEGF expression in granulosa-derived parenchymal lobule capillaries was correlated with the size of the luteal tissue, larger corpora lutea had higher expression, and tended to have a higher progesterone secretion. In conclusion, the current study indicates the existence of deleterious effects from exogenous progestagen treatments on progesterone secretion from induced corpora lutea, which correlate with alterations in the expression of VEGF in the luteal tissue and, this, presumably in the processes of neoangiogenesis and luteogenesis. © 2010 Blackwell Verlag GmbH.
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dig-inia-es-20.500.12792-23772020-12-15T09:52:43Z Deleterious effects of progestagen treatment in VEGF expression in corpora lutea of pregnant ewes Letelier, C. A. Sanchez, M. A. Garcia-Fernandez, R. A. Sanchez, B. Garcia-Palencia, P. Gonzalez-Bulnes, A. Flores, J. M. Contents The aim of the current study was to determine the possible effects of progestagen oestrous synchronization on vascular endothelial growth factor (VEGF) expression during sheep luteogenesis and the peri-implantation period and the relationship with luteal function. At days 9, 11, 13, 15, 17 and 21 of pregnancy, the ovaries from 30 progestagen treated and 30 ewes cycling after cloprostenol injection were evaluated by ultrasonography and, thereafter, collected and processed for immunohistochemical evaluation of VEGF; blood samples were drawn for evaluating plasma progesterone. The progestagen-treated group showed smaller corpora lutea than cloprostenol-treated and lower progesterone secretion. The expression of VEGF in the luteal cells increased with time in the cloprostenol group, but not in the progestagen-treated group, which even showed a decrease between days 11 and 13. In progestagen-treated sheep, VEGF expression in granulosa-derived parenchymal lobule capillaries was correlated with the size of the luteal tissue, larger corpora lutea had higher expression, and tended to have a higher progesterone secretion. In conclusion, the current study indicates the existence of deleterious effects from exogenous progestagen treatments on progesterone secretion from induced corpora lutea, which correlate with alterations in the expression of VEGF in the luteal tissue and, this, presumably in the processes of neoangiogenesis and luteogenesis. © 2010 Blackwell Verlag GmbH. 2020-10-22T12:53:49Z 2020-10-22T12:53:49Z 2011 journal article http://hdl.handle.net/20.500.12792/2377 10.1111/j.1439-0531.2010.01692.x eng Attribution-NonCommercial-ShareAlike 4.0 International http://creativecommons.org/licenses/by-nc-sa/4.0/ open access |
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Contents The aim of the current study was to determine the possible effects of progestagen oestrous synchronization on vascular endothelial growth factor (VEGF) expression during sheep luteogenesis and the peri-implantation period and the relationship with luteal function. At days 9, 11, 13, 15, 17 and 21 of pregnancy, the ovaries from 30 progestagen treated and 30 ewes cycling after cloprostenol injection were evaluated by ultrasonography and, thereafter, collected and processed for immunohistochemical evaluation of VEGF; blood samples were drawn for evaluating plasma progesterone. The progestagen-treated group showed smaller corpora lutea than cloprostenol-treated and lower progesterone secretion. The expression of VEGF in the luteal cells increased with time in the cloprostenol group, but not in the progestagen-treated group, which even showed a decrease between days 11 and 13. In progestagen-treated sheep, VEGF expression in granulosa-derived parenchymal lobule capillaries was correlated with the size of the luteal tissue, larger corpora lutea had higher expression, and tended to have a higher progesterone secretion. In conclusion, the current study indicates the existence of deleterious effects from exogenous progestagen treatments on progesterone secretion from induced corpora lutea, which correlate with alterations in the expression of VEGF in the luteal tissue and, this, presumably in the processes of neoangiogenesis and luteogenesis. © 2010 Blackwell Verlag GmbH. |
format |
journal article |
author |
Letelier, C. A. Sanchez, M. A. Garcia-Fernandez, R. A. Sanchez, B. Garcia-Palencia, P. Gonzalez-Bulnes, A. Flores, J. M. |
spellingShingle |
Letelier, C. A. Sanchez, M. A. Garcia-Fernandez, R. A. Sanchez, B. Garcia-Palencia, P. Gonzalez-Bulnes, A. Flores, J. M. Deleterious effects of progestagen treatment in VEGF expression in corpora lutea of pregnant ewes |
author_facet |
Letelier, C. A. Sanchez, M. A. Garcia-Fernandez, R. A. Sanchez, B. Garcia-Palencia, P. Gonzalez-Bulnes, A. Flores, J. M. |
author_sort |
Letelier, C. A. |
title |
Deleterious effects of progestagen treatment in VEGF expression in corpora lutea of pregnant ewes |
title_short |
Deleterious effects of progestagen treatment in VEGF expression in corpora lutea of pregnant ewes |
title_full |
Deleterious effects of progestagen treatment in VEGF expression in corpora lutea of pregnant ewes |
title_fullStr |
Deleterious effects of progestagen treatment in VEGF expression in corpora lutea of pregnant ewes |
title_full_unstemmed |
Deleterious effects of progestagen treatment in VEGF expression in corpora lutea of pregnant ewes |
title_sort |
deleterious effects of progestagen treatment in vegf expression in corpora lutea of pregnant ewes |
publishDate |
2011 |
url |
http://hdl.handle.net/20.500.12792/2377 |
work_keys_str_mv |
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