Sperm exposure to accessory gland secretions alters the transcriptomic response of the endometrium in cattle
9 Pàg.
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Other Authors: | |
| Format: | artículo biblioteca |
| Language: | English |
| Published: |
Elsevier
2024-04-01
|
| Subjects: | Accessory glands, Bull, Endometrium, Epididymis, Transcriptome, |
| Online Access: | http://hdl.handle.net/10261/350840 http://dx.doi.org/10.13039/501100001602 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100004837 https://api.elsevier.com/content/abstract/scopus_id/85183852136 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
dig-inia-es-10261-350840 |
|---|---|
| record_format |
koha |
| spelling |
dig-inia-es-10261-3508402024-10-26T20:47:59Z Sperm exposure to accessory gland secretions alters the transcriptomic response of the endometrium in cattle Sánchez, José María Rabaglino, M. B. Bagés Arnal, Sandra McDonald, Michael Behura, Susanta K. Spencer, T. E. Lonergan, P. Fernández-Fuertes, Beatriz European Commission Science Foundation Ireland Ministerio de Ciencia e Innovación (España) Sánchez, José María [0000-0003-3889-2342] Rabaglino, M. B. [0000-0002-0099-045X] Bagés Arnal, Sandra [0000-0003-3366-667X] Behura, Susanta K. [0000-0003-1057-2435] Spencer, T. E. [0000-0003-2815-766X] Fernández-Fuertes, Beatriz [0000-0003-2303-8733] Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] Accessory glands Bull Endometrium Epididymis Transcriptome 9 Pàg. In cattle, mating to intact, but not vasectomised, bulls has been shown to modify the endometrial transcriptome, suggesting an important role of sperm in the modulation of the uterine environment in this species. However, it is not clear whether these changes are driven by intrinsic sperm factors, or by factors of accessory gland (AG) origin that bind to sperm at ejaculation. Therefore, the aim of the present study was to determine whether ejaculated sperm, which are suspended in the secretions of the AGs, elicit a different endometrial transcriptomic response than epididymal sperm, which have never been exposed to AG factors. To this end, bovine endometrial explants collected from heifers in oestrus were (co-)incubated for 6 h alone (control), or with epididymal sperm or ejaculated sperm, following which transcriptomic changes in the endometrium were evaluated. Epididymal sperm elicited a more dramatic endometrial response than ejaculated sperm, in terms of the number of differentially expressed genes (DEGs). Indeed, RNA-sequencing data analysis revealed 1912 DEGs in endometrial explants exposed to epididymal sperm compared with control explants, whereas 115 DEGs were detected between endometrial explants exposed to ejaculated sperm in comparison to control explants. The top pathways associated with genes upregulated by epididymal sperm included T cell regulation and TNF, NF-KB and IL17 signalling. Interestingly, ejaculated sperm induced downregulation of genes associated with T cell immunity and Th17 differentiation, and upregulation of genes involved in NF-KB signalling, in comparison to epididymal sperm. These data indicate that factors of AG origin modulate the interaction between sperm and the endometrium in cattle. The authors wish to thank the National Cattle Breeding Centre for donating the fresh bull ejaculates used in this study, and Dr. John A. Browne (UCD) for his technical assistance. This project has received funding from the European Union's Horizon 2020 research and innovation program (Grant No. 792212); Science Foundation Ireland (Grant No. 16/IA/4474); and the Spanish Ministry of Science and Innovation (Grant No. IJC2019-039034-I). Peer reviewed 2024-03-18T17:15:41Z 2024-03-18T17:15:41Z 2024-04-01 artículo http://purl.org/coar/resource_type/c_6501 Theriogenology 218: 26-34 (2024) 0093-691X http://hdl.handle.net/10261/350840 10.1016/j.theriogenology.2024.01.037 1879-3231 http://dx.doi.org/10.13039/501100001602 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100004837 38295677 2-s2.0-85183852136 https://api.elsevier.com/content/abstract/scopus_id/85183852136 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/EC/H2020/792212 info:eu-repo/grantAgreement/MICINN//IJC2019-039034-I Departamento de Reproducción Animal Publisher's version https://doi.org/10.1016/j.theriogenology.2024.01.037 Sí open application/pdf Elsevier |
| institution |
INIA ES |
| collection |
DSpace |
| country |
España |
| countrycode |
ES |
| component |
Bibliográfico |
| access |
En linea |
| databasecode |
dig-inia-es |
| tag |
biblioteca |
| region |
Europa del Sur |
| libraryname |
Biblioteca del INIA España |
| language |
English |
| topic |
Accessory glands Bull Endometrium Epididymis Transcriptome Accessory glands Bull Endometrium Epididymis Transcriptome |
| spellingShingle |
Accessory glands Bull Endometrium Epididymis Transcriptome Accessory glands Bull Endometrium Epididymis Transcriptome Sánchez, José María Rabaglino, M. B. Bagés Arnal, Sandra McDonald, Michael Behura, Susanta K. Spencer, T. E. Lonergan, P. Fernández-Fuertes, Beatriz Sperm exposure to accessory gland secretions alters the transcriptomic response of the endometrium in cattle |
| description |
9 Pàg. |
| author2 |
European Commission |
| author_facet |
European Commission Sánchez, José María Rabaglino, M. B. Bagés Arnal, Sandra McDonald, Michael Behura, Susanta K. Spencer, T. E. Lonergan, P. Fernández-Fuertes, Beatriz |
| format |
artículo |
| topic_facet |
Accessory glands Bull Endometrium Epididymis Transcriptome |
| author |
Sánchez, José María Rabaglino, M. B. Bagés Arnal, Sandra McDonald, Michael Behura, Susanta K. Spencer, T. E. Lonergan, P. Fernández-Fuertes, Beatriz |
| author_sort |
Sánchez, José María |
| title |
Sperm exposure to accessory gland secretions alters the transcriptomic response of the endometrium in cattle |
| title_short |
Sperm exposure to accessory gland secretions alters the transcriptomic response of the endometrium in cattle |
| title_full |
Sperm exposure to accessory gland secretions alters the transcriptomic response of the endometrium in cattle |
| title_fullStr |
Sperm exposure to accessory gland secretions alters the transcriptomic response of the endometrium in cattle |
| title_full_unstemmed |
Sperm exposure to accessory gland secretions alters the transcriptomic response of the endometrium in cattle |
| title_sort |
sperm exposure to accessory gland secretions alters the transcriptomic response of the endometrium in cattle |
| publisher |
Elsevier |
| publishDate |
2024-04-01 |
| url |
http://hdl.handle.net/10261/350840 http://dx.doi.org/10.13039/501100001602 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100004837 https://api.elsevier.com/content/abstract/scopus_id/85183852136 |
| work_keys_str_mv |
AT sanchezjosemaria spermexposuretoaccessoryglandsecretionsaltersthetranscriptomicresponseoftheendometriumincattle AT rabaglinomb spermexposuretoaccessoryglandsecretionsaltersthetranscriptomicresponseoftheendometriumincattle AT bagesarnalsandra spermexposuretoaccessoryglandsecretionsaltersthetranscriptomicresponseoftheendometriumincattle AT mcdonaldmichael spermexposuretoaccessoryglandsecretionsaltersthetranscriptomicresponseoftheendometriumincattle AT behurasusantak spermexposuretoaccessoryglandsecretionsaltersthetranscriptomicresponseoftheendometriumincattle AT spencerte spermexposuretoaccessoryglandsecretionsaltersthetranscriptomicresponseoftheendometriumincattle AT lonerganp spermexposuretoaccessoryglandsecretionsaltersthetranscriptomicresponseoftheendometriumincattle AT fernandezfuertesbeatriz spermexposuretoaccessoryglandsecretionsaltersthetranscriptomicresponseoftheendometriumincattle |
| _version_ |
1816136618201841664 |