Antibiotic resistance genes as landscape anthropization indicators: Using a wild felid as sentinel in Chile
9 Pág.
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| Format: | artículo biblioteca |
| Language: | English |
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Elsevier BV
2020-02-10
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| Subjects: | Antimicrobial resistance, Bla(CTX-M), Landscape anthropization, Leopardus guigna, MecA, Public health, |
| Online Access: | http://hdl.handle.net/10261/343549 http://dx.doi.org/10.13039/100006363 http://dx.doi.org/10.13039/501100002850 http://dx.doi.org/10.13039/501100004630 https://api.elsevier.com/content/abstract/scopus_id/85075888234 |
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Antimicrobial resistance Bla(CTX-M) Landscape anthropization Leopardus guigna MecA Public health Antimicrobial resistance Bla(CTX-M) Landscape anthropization Leopardus guigna MecA Public health |
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Antimicrobial resistance Bla(CTX-M) Landscape anthropization Leopardus guigna MecA Public health Antimicrobial resistance Bla(CTX-M) Landscape anthropization Leopardus guigna MecA Public health Sacristán, Irene Esperón, Fernando Acuña, Francisca Aguilar, Emilio García, Sebastián López-Jara, María José Cevidanes, Aitor Neves, Elena Cabello-Stom, Javier Hidalgo-Hermoso, Ezequiel Poulin, Elie Millán, Javier Napolitano, Constanza Antibiotic resistance genes as landscape anthropization indicators: Using a wild felid as sentinel in Chile |
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9 Pág. |
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Fondo Nacional de Desarrollo Científico y Tecnológico (Chile) |
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Fondo Nacional de Desarrollo Científico y Tecnológico (Chile) Sacristán, Irene Esperón, Fernando Acuña, Francisca Aguilar, Emilio García, Sebastián López-Jara, María José Cevidanes, Aitor Neves, Elena Cabello-Stom, Javier Hidalgo-Hermoso, Ezequiel Poulin, Elie Millán, Javier Napolitano, Constanza |
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artículo |
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Antimicrobial resistance Bla(CTX-M) Landscape anthropization Leopardus guigna MecA Public health |
| author |
Sacristán, Irene Esperón, Fernando Acuña, Francisca Aguilar, Emilio García, Sebastián López-Jara, María José Cevidanes, Aitor Neves, Elena Cabello-Stom, Javier Hidalgo-Hermoso, Ezequiel Poulin, Elie Millán, Javier Napolitano, Constanza |
| author_sort |
Sacristán, Irene |
| title |
Antibiotic resistance genes as landscape anthropization indicators: Using a wild felid as sentinel in Chile |
| title_short |
Antibiotic resistance genes as landscape anthropization indicators: Using a wild felid as sentinel in Chile |
| title_full |
Antibiotic resistance genes as landscape anthropization indicators: Using a wild felid as sentinel in Chile |
| title_fullStr |
Antibiotic resistance genes as landscape anthropization indicators: Using a wild felid as sentinel in Chile |
| title_full_unstemmed |
Antibiotic resistance genes as landscape anthropization indicators: Using a wild felid as sentinel in Chile |
| title_sort |
antibiotic resistance genes as landscape anthropization indicators: using a wild felid as sentinel in chile |
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Elsevier BV |
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2020-02-10 |
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http://hdl.handle.net/10261/343549 http://dx.doi.org/10.13039/100006363 http://dx.doi.org/10.13039/501100002850 http://dx.doi.org/10.13039/501100004630 https://api.elsevier.com/content/abstract/scopus_id/85075888234 |
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dig-inia-es-10261-3435492024-05-16T20:42:53Z Antibiotic resistance genes as landscape anthropization indicators: Using a wild felid as sentinel in Chile Sacristán, Irene Esperón, Fernando Acuña, Francisca Aguilar, Emilio García, Sebastián López-Jara, María José Cevidanes, Aitor Neves, Elena Cabello-Stom, Javier Hidalgo-Hermoso, Ezequiel Poulin, Elie Millán, Javier Napolitano, Constanza Fondo Nacional de Desarrollo Científico y Tecnológico (Chile) Morris Animal Foundation National Geographic Society Mohamed bin Zayed Species Conservation Fund Australian Government Wild Felid Association Universidad Andrés Bello Sacristán, Irene [0000-0002-4169-4884] Esperón, Fernando [0000-0002-8810-5071] López-Jara, María José [0000-0002-9646-6483] Cevidanes, Aitor [0000-0003-1874-6399] Neves, Elena [0000-0002-1814-573X] Hidalgo-Hermoso, Ezequiel [0000-0002-1291-514X] Poulin, Elie [0000-0001-7736-0969] Millán, Javier [0000-0001-5608-781X] Napolitano, Constanza [0000-0002-7081-6975] Antimicrobial resistance Bla(CTX-M) Landscape anthropization Leopardus guigna MecA Public health 9 Pág. Antimicrobial resistance is a global emerging public health issue whose presence and impact in wildlife are widely unknown. Antimicrobial resistance genes (ARGs) are considered environmental contaminants, suitable to evaluate the degree of anthropic impact on wildlife and the environment. We used a wild felid, the guigna (Leopardus guigna), as a sentinel for the presence of ARGs in anthropized and pristine areas across their entire distribution range in Chile. We evaluated fecal samples from 51 wild guignas, collected between 2009 and 2018. Real-time PCR essays were employed to detect and quantify 22 selected ARGs in their fecal microbiome. All animals (100%) were positive for at least one ARG. The most prevalent ARG families were those that confer resistance to tetracycline (88.2%) and beta-lactamase (68.9%), with tet(Q) (60.8%), tet(W) (60.8%), and blaTEM (66.7%) as the most prevalent ARGs. Multi-resistance profiles were observed in 43% of the guignas. Statistically significant differences were found between anthropized and pristine areas for tet(Q) (p = 0.014), tet(W) (p = 0.0037), tetracycline family (p = 0.027), multi-resistance profile prevalence (p = 0.043) and tet(W) quantification (p = 0.004). Two animals from anthropized landscapes were positive for mecA, a gene associated with Staphylococcus aureus and other staphylococci resistant to methicillin, while three animals from anthropized areas were positive for blaCTX-M, that encodes class A extended-spectrum beta-lactamase. Both genes have been identified in bacteria causing relevant nosocomial infections worldwide. This is the first study on ARGs in wild felids from Chile and the first detection of mecA in South American wild felids. We observed an association between the degree of landscape anthropization and ARG prevalence, confirming that ARGs are important indicators of wildlife exposure to human activity/presence, with a widespread distribution. Our study was funded by CONICYT FONDECYT Iniciación, Chile 11150934 (CN), Morris Animal Foundation (MAF) Fellowship Training Award D15ZO-413 (CN), National Geographic Society Conservation Trust C309-15 (CN), Mohamed bin Zayed Species Conservation Fund 152510351 (CN), 2018 Endeavour Research Fellowship (Australian government) (CN), CONICYT PIA APOYO CCTE AFB170008 (CN, EP), the Wild Felid Association (IS), Fondo Interno UNAB Universidad Andres Bello, Chile DI-778-15/R (JM), Morris Animal Foundation D16Z-825 (JM), and CONICYT FONDECYT Regular 1161593 (JM, CN). Peer reviewed 2024-01-24T11:59:24Z 2024-01-24T11:59:24Z 2020-02-10 artículo http://purl.org/coar/resource_type/c_6501 Science of the Total Environment 703: e134900 (2020) 0048-9697 http://hdl.handle.net/10261/343549 10.1016/j.scitotenv.2019.134900 1879-1026 http://dx.doi.org/10.13039/100006363 http://dx.doi.org/10.13039/501100002850 http://dx.doi.org/10.13039/501100004630 31757538 2-s2.0-85075888234 https://api.elsevier.com/content/abstract/scopus_id/85075888234 en Centro de Investigación en Sanidad Animal (CISA) Publisher's version https://doi.org/10.1016/j.scitotenv.2019.134900 Sí open Elsevier BV |