Study of the biosynthesis and functionality of polyphosphate in Bifidobacterium longum KABP042
Polyphosphate (poly-P) biosynthesis in bacteria has been linked to many physiological processes and has been characterized as an interesting functional molecule involved in intestinal homeostasis. We determined the capacity for poly-P production of 18 probiotic strains mainly belonging to Bifidobacterium and former Lactobacillus genera, showing that poly-P synthesis varied widely between strains and is dependent on the availability of phosphate and the growth phase. Bifidobacteria were especially capable of poly-P synthesis and poly-P kinase (ppk) genes were identified in their genomes together with a repertoire of genes involved in phosphate transport and metabolism. In Bifidobacterium longum KABP042, the strain we found with highest poly-P production, variations in ppk expression were linked to growth conditions and presence of phosphate in the medium. Moreover, the strain produced poly-P in presence of breast milk and lacto-N-tetraose increased the amount of poly-P synthesized. Compared to KABP042 supernatants low in poly-P, exposure of Caco-2 cells to KABP042 supernatants rich in poly-P resulted in decreased epithelial permeability and increased barrier resistance, induction of epithelial protecting factors such as HSP27 and enhanced expression of tight junction protein genes. These results highlight the role of bifidobacteria-derived poly-P as a strain-dependent functional factor acting on epithelial integrity.
Main Authors: | , , , , , , , |
---|---|
Other Authors: | |
Format: | artículo biblioteca |
Language: | English |
Published: |
Springer Nature
2023-07-08
|
Subjects: | Bifidobacterium, Bifidobacterium longum, Metabolism, Caco-2 Cells, Polyphosphates, Humans, Intestines, Microbiology, Probiotics, |
Online Access: | http://hdl.handle.net/10261/331642 http://dx.doi.org/10.13039/501100001872 https://api.elsevier.com/content/abstract/scopus_id/85164163342 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-inia-es-10261-331642 |
---|---|
record_format |
koha |
spelling |
dig-inia-es-10261-3316422024-05-17T20:41:26Z Study of the biosynthesis and functionality of polyphosphate in Bifidobacterium longum KABP042 Alcántara, Cristina Perez, Marta Huedo, Pol Altadill, Tatiana Espadaler Mazo, Jordi Arqués, Juan Luis Zúñiga, Manuel Monedero, Vicente Centro para el Desarrollo Tecnológico Industrial (España) #NODATA# #NODATA# #NODATA# #NODATA# #NODATA# #NODATA# #NODATA# #NODATA# Bifidobacterium Bifidobacterium longum Metabolism Caco-2 Cells Polyphosphates Humans Intestines Microbiology Probiotics Polyphosphate (poly-P) biosynthesis in bacteria has been linked to many physiological processes and has been characterized as an interesting functional molecule involved in intestinal homeostasis. We determined the capacity for poly-P production of 18 probiotic strains mainly belonging to Bifidobacterium and former Lactobacillus genera, showing that poly-P synthesis varied widely between strains and is dependent on the availability of phosphate and the growth phase. Bifidobacteria were especially capable of poly-P synthesis and poly-P kinase (ppk) genes were identified in their genomes together with a repertoire of genes involved in phosphate transport and metabolism. In Bifidobacterium longum KABP042, the strain we found with highest poly-P production, variations in ppk expression were linked to growth conditions and presence of phosphate in the medium. Moreover, the strain produced poly-P in presence of breast milk and lacto-N-tetraose increased the amount of poly-P synthesized. Compared to KABP042 supernatants low in poly-P, exposure of Caco-2 cells to KABP042 supernatants rich in poly-P resulted in decreased epithelial permeability and increased barrier resistance, induction of epithelial protecting factors such as HSP27 and enhanced expression of tight junction protein genes. These results highlight the role of bifidobacteria-derived poly-P as a strain-dependent functional factor acting on epithelial integrity. The authors gratefully acknowledge the financial support of this work by the Center for the Development of Industrial Technology (CDTI) of the Spanish Ministry of Science and Innovation (project number IDI-20220028). Peer reviewed 2023-07-21T12:53:46Z 2023-07-21T12:53:46Z 2023-07-08 artículo http://purl.org/coar/resource_type/c_6501 Scientific Reports 13 (1): 11076 (2023) http://hdl.handle.net/10261/331642 10.1038/s41598-023-38082-0 2045-2322 http://dx.doi.org/10.13039/501100001872 37422465 2-s2.0-85164163342 https://api.elsevier.com/content/abstract/scopus_id/85164163342 en Scientific reports Publisher's version https://doi.org/10.1038/s41598-023-38082-0 Sí open Springer Nature |
institution |
INIA ES |
collection |
DSpace |
country |
España |
countrycode |
ES |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-inia-es |
tag |
biblioteca |
region |
Europa del Sur |
libraryname |
Biblioteca del INIA España |
language |
English |
topic |
Bifidobacterium Bifidobacterium longum Metabolism Caco-2 Cells Polyphosphates Humans Intestines Microbiology Probiotics Bifidobacterium Bifidobacterium longum Metabolism Caco-2 Cells Polyphosphates Humans Intestines Microbiology Probiotics |
spellingShingle |
Bifidobacterium Bifidobacterium longum Metabolism Caco-2 Cells Polyphosphates Humans Intestines Microbiology Probiotics Bifidobacterium Bifidobacterium longum Metabolism Caco-2 Cells Polyphosphates Humans Intestines Microbiology Probiotics Alcántara, Cristina Perez, Marta Huedo, Pol Altadill, Tatiana Espadaler Mazo, Jordi Arqués, Juan Luis Zúñiga, Manuel Monedero, Vicente Study of the biosynthesis and functionality of polyphosphate in Bifidobacterium longum KABP042 |
description |
Polyphosphate (poly-P) biosynthesis in bacteria has been linked to many physiological processes and has been characterized as an interesting functional molecule involved in intestinal homeostasis. We determined the capacity for poly-P production of 18 probiotic strains mainly belonging to Bifidobacterium and former Lactobacillus genera, showing that poly-P synthesis varied widely between strains and is dependent on the availability of phosphate and the growth phase. Bifidobacteria were especially capable of poly-P synthesis and poly-P kinase (ppk) genes were identified in their genomes together with a repertoire of genes involved in phosphate transport and metabolism. In Bifidobacterium longum KABP042, the strain we found with highest poly-P production, variations in ppk expression were linked to growth conditions and presence of phosphate in the medium. Moreover, the strain produced poly-P in presence of breast milk and lacto-N-tetraose increased the amount of poly-P synthesized. Compared to KABP042 supernatants low in poly-P, exposure of Caco-2 cells to KABP042 supernatants rich in poly-P resulted in decreased epithelial permeability and increased barrier resistance, induction of epithelial protecting factors such as HSP27 and enhanced expression of tight junction protein genes. These results highlight the role of bifidobacteria-derived poly-P as a strain-dependent functional factor acting on epithelial integrity. |
author2 |
Centro para el Desarrollo Tecnológico Industrial (España) |
author_facet |
Centro para el Desarrollo Tecnológico Industrial (España) Alcántara, Cristina Perez, Marta Huedo, Pol Altadill, Tatiana Espadaler Mazo, Jordi Arqués, Juan Luis Zúñiga, Manuel Monedero, Vicente |
format |
artículo |
topic_facet |
Bifidobacterium Bifidobacterium longum Metabolism Caco-2 Cells Polyphosphates Humans Intestines Microbiology Probiotics |
author |
Alcántara, Cristina Perez, Marta Huedo, Pol Altadill, Tatiana Espadaler Mazo, Jordi Arqués, Juan Luis Zúñiga, Manuel Monedero, Vicente |
author_sort |
Alcántara, Cristina |
title |
Study of the biosynthesis and functionality of polyphosphate in Bifidobacterium longum KABP042 |
title_short |
Study of the biosynthesis and functionality of polyphosphate in Bifidobacterium longum KABP042 |
title_full |
Study of the biosynthesis and functionality of polyphosphate in Bifidobacterium longum KABP042 |
title_fullStr |
Study of the biosynthesis and functionality of polyphosphate in Bifidobacterium longum KABP042 |
title_full_unstemmed |
Study of the biosynthesis and functionality of polyphosphate in Bifidobacterium longum KABP042 |
title_sort |
study of the biosynthesis and functionality of polyphosphate in bifidobacterium longum kabp042 |
publisher |
Springer Nature |
publishDate |
2023-07-08 |
url |
http://hdl.handle.net/10261/331642 http://dx.doi.org/10.13039/501100001872 https://api.elsevier.com/content/abstract/scopus_id/85164163342 |
work_keys_str_mv |
AT alcantaracristina studyofthebiosynthesisandfunctionalityofpolyphosphateinbifidobacteriumlongumkabp042 AT perezmarta studyofthebiosynthesisandfunctionalityofpolyphosphateinbifidobacteriumlongumkabp042 AT huedopol studyofthebiosynthesisandfunctionalityofpolyphosphateinbifidobacteriumlongumkabp042 AT altadilltatiana studyofthebiosynthesisandfunctionalityofpolyphosphateinbifidobacteriumlongumkabp042 AT espadalermazojordi studyofthebiosynthesisandfunctionalityofpolyphosphateinbifidobacteriumlongumkabp042 AT arquesjuanluis studyofthebiosynthesisandfunctionalityofpolyphosphateinbifidobacteriumlongumkabp042 AT zunigamanuel studyofthebiosynthesisandfunctionalityofpolyphosphateinbifidobacteriumlongumkabp042 AT monederovicente studyofthebiosynthesisandfunctionalityofpolyphosphateinbifidobacteriumlongumkabp042 |
_version_ |
1802819772903063552 |