Non-coding RNAs derived from the foot-and-mouth disease virus genome trigger broad antiviral activity against coronaviruses
12 Pág.
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| Main Authors: | , , , , , , , , , |
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| Format: | artículo biblioteca |
| Language: | English |
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Frontiers Media
2023-03-29
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| Subjects: | COVID-19, RNA-based therapy, SARS-CoV-2, Antiviral immunity, Coronaviruses, Foot-and-mouth disease virus (FMDV), Non-coding RNA, Type-I IFN, |
| Online Access: | http://hdl.handle.net/10261/330229 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100000780 https://api.elsevier.com/content/abstract/scopus_id/85152628012 |
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dig-inia-es-10261-3302292024-05-18T20:40:56Z Non-coding RNAs derived from the foot-and-mouth disease virus genome trigger broad antiviral activity against coronaviruses Rodríguez Pulido, Miguel Ramón Calvo Pinilla, Eva María Polo, Miryam Saiz Calahorra, Juan Carlos Fernández González, Raúl Pericuesta Camacho, Eva Gutiérrez-Adán, Alfonso Sobrino Castelló, Francisco Martín-Acebes, M. A. Sáiz, Margarita European Commission CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) Ministerio de Ciencia e Innovación (España) Rodríguez Pulido, Miguel Ramón [0000-0003-2101-8974] Calvo Pinilla, Eva María [0000-0002-4667-4081] Polo, Miryam [0000-0002-5222-2313] Saiz Calahorra, Juan Carlos [0000-0001-8269-5544] Fernández González, Raúl [0000-0003-1989-2945] Pericuesta Camacho, Eva [0000-0001-9387-3270] Gutiérrez-Adán, Alfonso [0000-0001-9893-9179] Sobrino, F. [0000-0003-4968-8291] Martín-Acebes, M. A. [0000-0001-6015-3613] Sáiz, Margarita [0000-0003-3893-7926] COVID-19 RNA-based therapy SARS-CoV-2 Antiviral immunity Coronaviruses Foot-and-mouth disease virus (FMDV) Non-coding RNA Type-I IFN 12 Pág. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of a potentially severe respiratory disease, the coronavirus disease 2019 (COVID-19), an ongoing pandemic with limited therapeutic options. Here, we assessed the anti-coronavirus activity of synthetic RNAs mimicking specific domains in the non-coding regions of the foot-and-mouth disease virus (FMDV) genome (ncRNAs). These molecules are known to exert broad-spectrum antiviral activity in cell culture, mice and pigs effectively triggering the host innate immune response. The ncRNAs showed potent antiviral activity against SARS-CoV-2 after transfection in human intestinal Caco-2 and lung epithelium Calu-3 2B4 cells. When the in vivo efficacy of the FMDV ncRNAs was assessed in K18-hACE2 mice, administration of naked ncRNA before intranasal SARS-CoV-2 infection significantly decreased the viral load and the levels of pro-inflammatory cytokines in the lungs compared with untreated infected mice. The ncRNAs were also highly efficacious when assayed against common human HCoV-229E and porcine transmissible gastroenteritis virus (TGEV) in hepatocyte-derived Huh-7 and swine testis ST cells, respectively. These results are a proof of concept of the pan-coronavirus antiviral activity of the FMDV ncRNAs including human and animal divergent coronaviruses and potentially enhance our ability to fight future emerging variants. This research work was funded by the European Commission – NextGenerationEU (Regulation EU 2020/2094), through CSIC´s Global Health Platform (PTI Salud Global), grants SGL 2103051 (to MS and FS) and SGL2103053 (to MM-A). It was further supported by the Spanish Ministry of Science and Innovation, grant PID2020-113184RB-C21 (to FS and MS) and by grant S018/BAA-4370 (co-financed by the Autonomous Community of Madrid and EC FEDER funds, to FS). MP was the recipient of a scholarship from the CSIC´s JAE Intro program. Peer reviewed 2023-07-03T09:13:48Z 2023-07-03T09:13:48Z 2023-03-29 artículo http://purl.org/coar/resource_type/c_6501 Frontiers in Immunology 14: e1166725 (2023) http://hdl.handle.net/10261/330229 10.3389/fimmu.2023.1166725 1664-3224 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100000780 37063925 2-s2.0-85152628012 https://api.elsevier.com/content/abstract/scopus_id/85152628012 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/EC//SGL 2103051 info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113184RB-C21/ES/VACUNAS PEPTIDICAS, ESTRATEGIAS ANTIVIRALES Y MECANISMOS DE EVASION INMUNE. APLICACIONES BIOTERAPEUTICAS/ S018/BAA-4370 Frontiers in immunology Publisher's version https://doi.org/10.3389/fimmu.2023.1166725 Sí open application/pdf Frontiers Media |
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COVID-19 RNA-based therapy SARS-CoV-2 Antiviral immunity Coronaviruses Foot-and-mouth disease virus (FMDV) Non-coding RNA Type-I IFN COVID-19 RNA-based therapy SARS-CoV-2 Antiviral immunity Coronaviruses Foot-and-mouth disease virus (FMDV) Non-coding RNA Type-I IFN |
| spellingShingle |
COVID-19 RNA-based therapy SARS-CoV-2 Antiviral immunity Coronaviruses Foot-and-mouth disease virus (FMDV) Non-coding RNA Type-I IFN COVID-19 RNA-based therapy SARS-CoV-2 Antiviral immunity Coronaviruses Foot-and-mouth disease virus (FMDV) Non-coding RNA Type-I IFN Rodríguez Pulido, Miguel Ramón Calvo Pinilla, Eva María Polo, Miryam Saiz Calahorra, Juan Carlos Fernández González, Raúl Pericuesta Camacho, Eva Gutiérrez-Adán, Alfonso Sobrino Castelló, Francisco Martín-Acebes, M. A. Sáiz, Margarita Non-coding RNAs derived from the foot-and-mouth disease virus genome trigger broad antiviral activity against coronaviruses |
| description |
12 Pág. |
| author2 |
European Commission |
| author_facet |
European Commission Rodríguez Pulido, Miguel Ramón Calvo Pinilla, Eva María Polo, Miryam Saiz Calahorra, Juan Carlos Fernández González, Raúl Pericuesta Camacho, Eva Gutiérrez-Adán, Alfonso Sobrino Castelló, Francisco Martín-Acebes, M. A. Sáiz, Margarita |
| format |
artículo |
| topic_facet |
COVID-19 RNA-based therapy SARS-CoV-2 Antiviral immunity Coronaviruses Foot-and-mouth disease virus (FMDV) Non-coding RNA Type-I IFN |
| author |
Rodríguez Pulido, Miguel Ramón Calvo Pinilla, Eva María Polo, Miryam Saiz Calahorra, Juan Carlos Fernández González, Raúl Pericuesta Camacho, Eva Gutiérrez-Adán, Alfonso Sobrino Castelló, Francisco Martín-Acebes, M. A. Sáiz, Margarita |
| author_sort |
Rodríguez Pulido, Miguel Ramón |
| title |
Non-coding RNAs derived from the foot-and-mouth disease virus genome trigger broad antiviral activity against coronaviruses |
| title_short |
Non-coding RNAs derived from the foot-and-mouth disease virus genome trigger broad antiviral activity against coronaviruses |
| title_full |
Non-coding RNAs derived from the foot-and-mouth disease virus genome trigger broad antiviral activity against coronaviruses |
| title_fullStr |
Non-coding RNAs derived from the foot-and-mouth disease virus genome trigger broad antiviral activity against coronaviruses |
| title_full_unstemmed |
Non-coding RNAs derived from the foot-and-mouth disease virus genome trigger broad antiviral activity against coronaviruses |
| title_sort |
non-coding rnas derived from the foot-and-mouth disease virus genome trigger broad antiviral activity against coronaviruses |
| publisher |
Frontiers Media |
| publishDate |
2023-03-29 |
| url |
http://hdl.handle.net/10261/330229 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100000780 https://api.elsevier.com/content/abstract/scopus_id/85152628012 |
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1802819761281695744 |