NMR Study on Laccase Polymerization of Kraft Lignin Using Different Enzymes Source
17 Pág.
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| Format: | artículo biblioteca |
| Language: | English |
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Multidisciplinary Digital Publishing Institute
2023-01-25
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| Subjects: | Kraft lignin, NMR characterization, Eucalypt, Laccase, Polymerization, |
| Online Access: | http://hdl.handle.net/10261/330212 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/100012818 https://api.elsevier.com/content/abstract/scopus_id/85147894135 |
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dig-inia-es-10261-3302122024-05-14T20:44:26Z NMR Study on Laccase Polymerization of Kraft Lignin Using Different Enzymes Source Ibarra Trejo, David García-Fuentevilla, Luisa Domínguez, Gabriela Martín-Sampedro, R. Hernández, Manuel Arias, María Enriqueta Santos, José I. Eugenio Martín, María Eugenia Comunidad de Madrid Agencia Estatal de Investigación (España) Ministerio de Ciencia e Innovación (España) Ibarra Trejo, David [0000-0003-3001-7308] García-Fuentevilla, Luisa [0000-0001-8255-0097] Domínguez, Gabriela [0000-0001-7358-0913] Martín-Sampedro, R. [0000-0003-2083-3723] Arias, María Enriqueta [0000-0002-6804-1779] Santos, José I. [0000-0002-5199-2332] Eugenio Martín, María Eugenia [0000-0003-4550-5400] Kraft lignin NMR characterization Eucalypt Laccase Polymerization 17 Pág. The usage of laccases is a sustainable and environmentally friendly approach to modifying the Kraft lignin structure for use in certain applications. However, the inherent structure of Kraft lignin, as well as that resulting from laccase modification, still presents challenges for fundamental comprehension and successful lignin valorization. In this study, bacterial and fungal laccases were employed to modify eucalypt Kraft lignin. To evaluate the type and range of the chemical and structural changes of laccase-treated lignins, different NMR techniques, including solution 1H and 2D NMR (heteronuclear single quantum correlation (HSQC)), and solid-state 13C NMR, were applied. Size exclusion chromatography and infrared spectroscopy were also used. Interestingly, HSQC analysis showed substantial changes in the oxygenated aliphatic region of lignins, showing an almost complete absence of signals corresponding to side-chains due to laccase depolymerization. Simultaneously, a significant loss of aromatic signals was observed by HSQC and 1H NMR, which was attributed to a deprotonation of the lignin benzenic rings due to polymerization/condensation by laccase reactions. Then, condensed structures, such as α-5', 5-5', and 4-O-5', were detected by HSQC and 13C NMR, supporting the increment in molecular weight, as well as the phenolic content reduction determined in lignins. This research was funded by Comunidad de Madrid via Project SUSTEC-CM S2018/EMT-4348; MCINN/AEI/10.13039/501100011033 and by “ERDF A way of making Europe” via Project RTI2018-096080-B-C22; and MCINN via Project TED2021-132122B-C21. Peer reviewed 2023-07-03T07:31:08Z 2023-07-03T07:31:08Z 2023-01-25 artículo http://purl.org/coar/resource_type/c_6501 International Journal of Molecular Sciences 24(3): e2359 (2023) 1661-6596 http://hdl.handle.net/10261/330212 10.3390/ijms24032359 1422-0067 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/100012818 36768678 2-s2.0-85147894135 https://api.elsevier.com/content/abstract/scopus_id/85147894135 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# S2018/EMT-4348 info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-096080-B-C22/ES/OBTENCION, MODIFICACION Y CARACTERIZACION DE DIFERENTES LIGNINAS RESIDUALES PARA LA PRODUCCION DE NANOFIBRAS POR ELECTROSPINNING/ info:eu-repo/grantAgreement/MCINN//TED2021-132122B-C21 Instituto de Ciencias Forestales (ICIFOR) Publisher's version https://doi.org/10.3390/ijms24032359 Sí open application/pdf Multidisciplinary Digital Publishing Institute |
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Kraft lignin NMR characterization Eucalypt Laccase Polymerization Kraft lignin NMR characterization Eucalypt Laccase Polymerization |
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Kraft lignin NMR characterization Eucalypt Laccase Polymerization Kraft lignin NMR characterization Eucalypt Laccase Polymerization Ibarra Trejo, David García-Fuentevilla, Luisa Domínguez, Gabriela Martín-Sampedro, R. Hernández, Manuel Arias, María Enriqueta Santos, José I. Eugenio Martín, María Eugenia NMR Study on Laccase Polymerization of Kraft Lignin Using Different Enzymes Source |
| description |
17 Pág. |
| author2 |
Comunidad de Madrid |
| author_facet |
Comunidad de Madrid Ibarra Trejo, David García-Fuentevilla, Luisa Domínguez, Gabriela Martín-Sampedro, R. Hernández, Manuel Arias, María Enriqueta Santos, José I. Eugenio Martín, María Eugenia |
| format |
artículo |
| topic_facet |
Kraft lignin NMR characterization Eucalypt Laccase Polymerization |
| author |
Ibarra Trejo, David García-Fuentevilla, Luisa Domínguez, Gabriela Martín-Sampedro, R. Hernández, Manuel Arias, María Enriqueta Santos, José I. Eugenio Martín, María Eugenia |
| author_sort |
Ibarra Trejo, David |
| title |
NMR Study on Laccase Polymerization of Kraft Lignin Using Different Enzymes Source |
| title_short |
NMR Study on Laccase Polymerization of Kraft Lignin Using Different Enzymes Source |
| title_full |
NMR Study on Laccase Polymerization of Kraft Lignin Using Different Enzymes Source |
| title_fullStr |
NMR Study on Laccase Polymerization of Kraft Lignin Using Different Enzymes Source |
| title_full_unstemmed |
NMR Study on Laccase Polymerization of Kraft Lignin Using Different Enzymes Source |
| title_sort |
nmr study on laccase polymerization of kraft lignin using different enzymes source |
| publisher |
Multidisciplinary Digital Publishing Institute |
| publishDate |
2023-01-25 |
| url |
http://hdl.handle.net/10261/330212 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/100012818 https://api.elsevier.com/content/abstract/scopus_id/85147894135 |
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