Transforming growth factor beta (TGFβ) pathway is essential for hypoblast and epiblast development in ovine post-hatching embryos
9 Pág.
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Format: | artículo biblioteca |
Language: | English |
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Elsevier
2023-01-15
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Subjects: | Embryo, Ovine, Post-blastocyst in vitro culture, ROCK inhibitor, TGFβ, |
Online Access: | http://hdl.handle.net/10261/308360 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100004837 https://api.elsevier.com/content/abstract/scopus_id/85142454200 |
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dig-inia-es-10261-3083602024-05-15T20:42:39Z Transforming growth factor beta (TGFβ) pathway is essential for hypoblast and epiblast development in ovine post-hatching embryos Galiano-Cogolludo, Beatriz Marigorta, Pilar Yus Girón, Víctor Bermejo Álvarez, Pablo Ramos Ibeas, Priscila European Commission Ministerio de Ciencia e Innovación (España) Galiano-Cogolludo, Beatriz [0000-0003-4687-8583] Marigorta, Pilar [0000-0002-6287-5780] Bermejo Álvarez, Pablo [0000-0001-9907-2626] Ramos Ibeas, Priscila [0000-0003-4578-0189] Bermejo Álvarez, Pablo [0000-0001-9907-2626] Embryo Ovine Post-blastocyst in vitro culture ROCK inhibitor TGFβ 9 Pág. The developmental failures occurring between blastocyst hatching and implantation in farm ungulates are a major cause of pregnancy losses. At the expanded blastocyst stage, three cell lineages emerge in the embryo: trophoblast, hypoblast and epiblast, the latter being the most vulnerable during post-hatching development. Transforming growth factor beta (TGFβ) signaling pathway is involved in hypoblast and epiblast development; however, previous in vitro functional studies are limited to the expanded blastocyst stage. In this study, we have analyzed the effect of TGFβ inhibition with 10, 20 or 40 μM SB431542 during ovine post-hatching developmental period using a recently developed culture system able to recapitulate major developmental landmarks. We have found a negative effect of TGFβ inhibition on hypoblast and epiblast development that could be partially reverted by Rho-associated protein kinase (ROCK) inhibitor Y-27632. Our findings provide new insights into the molecular networks regulating embryo development beyond the expanded blastocyst and could help to elucidate the causes of early pregnancy losses in farm ungulates. This work has been funded by the projects StG-757886-ELONGAN from the European Research Council to PBA and PID2021-122153NA-I00 from the Spanish Ministry of Science and Innovation to PRI. Peer reviewed 2023-05-08T08:58:54Z 2023-05-08T08:58:54Z 2023-01-15 artículo http://purl.org/coar/resource_type/c_6501 Theriogenology 196: 112-120 (2023) 1879-3231 http://hdl.handle.net/10261/308360 10.1016/j.theriogenology.2022.11.021 0093-691X http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100004837 36413867 2-s2.0-85142454200 https://api.elsevier.com/content/abstract/scopus_id/85142454200 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/EC//757886 info:eu-repo/grantAgreement/MICINN//PID2021-122153NA-I00 Departamento de Reproducción Animal Publisher's version https://doi.org/10.1016/j.theriogenology.2022.11.021 Sí open Elsevier |
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Embryo Ovine Post-blastocyst in vitro culture ROCK inhibitor TGFβ Embryo Ovine Post-blastocyst in vitro culture ROCK inhibitor TGFβ |
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Embryo Ovine Post-blastocyst in vitro culture ROCK inhibitor TGFβ Embryo Ovine Post-blastocyst in vitro culture ROCK inhibitor TGFβ Galiano-Cogolludo, Beatriz Marigorta, Pilar Yus Girón, Víctor Bermejo Álvarez, Pablo Ramos Ibeas, Priscila Transforming growth factor beta (TGFβ) pathway is essential for hypoblast and epiblast development in ovine post-hatching embryos |
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9 Pág. |
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European Commission |
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European Commission Galiano-Cogolludo, Beatriz Marigorta, Pilar Yus Girón, Víctor Bermejo Álvarez, Pablo Ramos Ibeas, Priscila |
format |
artículo |
topic_facet |
Embryo Ovine Post-blastocyst in vitro culture ROCK inhibitor TGFβ |
author |
Galiano-Cogolludo, Beatriz Marigorta, Pilar Yus Girón, Víctor Bermejo Álvarez, Pablo Ramos Ibeas, Priscila |
author_sort |
Galiano-Cogolludo, Beatriz |
title |
Transforming growth factor beta (TGFβ) pathway is essential for hypoblast and epiblast development in ovine post-hatching embryos |
title_short |
Transforming growth factor beta (TGFβ) pathway is essential for hypoblast and epiblast development in ovine post-hatching embryos |
title_full |
Transforming growth factor beta (TGFβ) pathway is essential for hypoblast and epiblast development in ovine post-hatching embryos |
title_fullStr |
Transforming growth factor beta (TGFβ) pathway is essential for hypoblast and epiblast development in ovine post-hatching embryos |
title_full_unstemmed |
Transforming growth factor beta (TGFβ) pathway is essential for hypoblast and epiblast development in ovine post-hatching embryos |
title_sort |
transforming growth factor beta (tgfβ) pathway is essential for hypoblast and epiblast development in ovine post-hatching embryos |
publisher |
Elsevier |
publishDate |
2023-01-15 |
url |
http://hdl.handle.net/10261/308360 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100004837 https://api.elsevier.com/content/abstract/scopus_id/85142454200 |
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