Haploids in conifer species Characterization and chromosomal integrity of a maritime pine cell line

Haploids are a valuable tool for genomic studies in higher plants, especially those with huge genome size and long juvenile periods, such as conifers. In these species, megagametophyte cultures have been widely used to obtain haploid callus and somatic embryogenic lines. One of the main problems associated with tissue culture is the potential genetic instability of the regenerants. Because of this, chromosomal stability of the callus and/or somatic embryos should also be assessed. To this end, chromosome counting, flow cytometry and genotyping using microsatellites have been reported. Here, we present an overview of the work done in conifers, with special emphasis on the production of a haploid cell line in maritime pine (Pinus pinaster L.) and the use of a set of molecular markers, which includes Single Nucleotide Polymorphisms (SNPs) and microsatellites or Single Sequence Repeats (SSRs), to validate chromosomal integrity confirming the presence of all chromosomic arms. © 2016 by the authors.

Bibliographic Details

Main Authors:	Cabezas Martínez, José Antonio, Morcillo, M., Vélez, M. D., Díaz, L., Segura, J., Cervera, M. T., Arrillaga, I.
Format:	journal article biblioteca
Language:	English
Published:	Multidisciplinary Digital Publishing Institute 2016
Subjects:	Genetic stability, Genome sequencing, Haploid tissue, Molecular markers, Pinus pinaster,
Online Access:	http://hdl.handle.net/20.500.12792/5240 http://hdl.handle.net/10261/294936
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