In situ random primer extension of metaphase chromosomes
We have developed a technique of random primer extension of fixed chromosomes that is applicable to both mouse and man. Human chromosomes are not homogeneously labeled with this technique; those regions corresponding to R-bands appear to be more sensitive than those identified as G-bands, whereas centromeric regions are not labeled. These results not only corroborate specific structural differences between distinct regions of mammalian genomes but also open up the possibility of assays with specific primers to test whether primer extension is useful for the identification of genes and families of sequences on chromosomes.
Saved in:
Main Authors: | García de la Vega, C., Martínez Zapater, J. M., López-Fernández, C., Goyanes, V., Mezzanotte, R., Gosálvez, J. |
---|---|
Format: | journal article biblioteca |
Language: | English |
Published: |
S. Karger AG
1990
|
Online Access: | http://hdl.handle.net/20.500.12792/5111 http://hdl.handle.net/10261/294893 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
In situ random primer extension of metaphase chromosomes
by: García de la Vega, C., et al.
Published: (1990) -
Non-random distribution of high density chromatin detected at opposite ends of T-banded human metaphase chromosomes
by: Santiñaque,Federico F., et al.
Published: (2007) -
A method to increase mitotic metaphase spreads in permanent chromosome preparations for karyotype studies of fishes
by: Reddy, P.V.G.K., et al.
Published: (1987) -
In vitro embryos production in bovine after metaphase plate nuclear transfer
by: CAIXETA, F. M. C., et al.
Published: (2018-01-16) -
Metaphase cytogenetics and single nucleotide polymorphism arrays in myeloid malignancies
by: Silva,Fernanda Borges da, et al.
Published: (2015)