DNA methylation increases throughout Arabidopsis development
We used amplified fragment length polymorphisms (AFLP) to analyze the stability of DNA methylation throughout Arabidopsis development. AFLP can detect genome-wide changes in cytosine methylation produced by DNA demethylation agents, such as 5-azacytidine, or specific mutations at the DDM1 locus. In both cases, cytosine demethylation is associated with a general increase in the presence of amplified fragments. Using this approach, we followed DNA methylation at methylation sensitive restriction sites throughout Arabidopsis development. The results show a progressive DNA methylation trend from cotyledons to vegetative organs to reproductive organs. © Springer-Verlag 2005.
Main Authors: | , , |
---|---|
Format: | journal article biblioteca |
Language: | English |
Published: |
Springer
2005
|
Subjects: | Arabidopsis, 5-azacytidine, ddm1 mutant, DNA methylation pattern, Plant development, |
Online Access: | http://hdl.handle.net/20.500.12792/3934 http://hdl.handle.net/10261/294493 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|