Foot-and-mouth disease virus infection induces proteolytic cleavage of PTB, eIF3a,b, and PABP RNA-binding proteins

Foot-and-mouth disease virus infection induces proteolytic cleavage of PTB, eIF3a,b, and PABP RNA-binding proteins

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Foot-and-mouth disease virus (FMDV) infection induces major changes in the host cell including the shutoff of cellular protein synthesis. Here, protein extracts from FMDV-infected cells have been used to monitor changes in the profile of RNA-binding factors interacting with regulatory regions of the viral RNA. Relevant differences have been detected in the pattern of interaction with proteins prepared from either infected or uninfected cells with RNA probes encompassing the internal ribosome entry site (IRES), the 5′ and 3′end regions. The binding patterns obtained for two divergent FMDV isolates showed differences depending on the viral isolate used. The identity of the host proteins giving a shifted binding pattern to RNA regulatory regions has been inferred by immunoblotting. Our results show that polypyrimidine tract-binding protein (PTB) and two subunits of translation initiation factor eIF3 interacting with the IRES undergo proteolytic processing during FMDV infection. In addition, poly(A)-binding protein (PABP), interacting with the 3′end of the viral RNA is partially processed. Proteolysis of eIF3a, eIF3b, PABP and PTB correlated with the extent of cytopathic effect induced by FMDV in infected cells. © 2007 Elsevier Inc. All rights reserved.

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Main Authors: Rodríguez Pulido, M., Serrano, P., Sáiz, M., Martínez-Salas, E.
Format: journal article biblioteca
Language:English
Published: Elsevier 2007
Subjects:FMDV, RNA-binding proteins, IRES, S region, 3′end, eIF3, PTB, PABP, Proteolytic cleavage, Infected cells,
Online Access:http://hdl.handle.net/20.500.12792/3812
http://hdl.handle.net/10261/294448
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spelling dig-inia-es-10261-2944482023-02-20T10:38:50Z Foot-and-mouth disease virus infection induces proteolytic cleavage of PTB, eIF3a,b, and PABP RNA-binding proteins Rodríguez Pulido, M. Serrano, P. Sáiz, M. Martínez-Salas, E. FMDV RNA-binding proteins IRES S region 3′end eIF3 PTB PABP Proteolytic cleavage Infected cells Foot-and-mouth disease virus (FMDV) infection induces major changes in the host cell including the shutoff of cellular protein synthesis. Here, protein extracts from FMDV-infected cells have been used to monitor changes in the profile of RNA-binding factors interacting with regulatory regions of the viral RNA. Relevant differences have been detected in the pattern of interaction with proteins prepared from either infected or uninfected cells with RNA probes encompassing the internal ribosome entry site (IRES), the 5′ and 3′end regions. The binding patterns obtained for two divergent FMDV isolates showed differences depending on the viral isolate used. The identity of the host proteins giving a shifted binding pattern to RNA regulatory regions has been inferred by immunoblotting. Our results show that polypyrimidine tract-binding protein (PTB) and two subunits of translation initiation factor eIF3 interacting with the IRES undergo proteolytic processing during FMDV infection. In addition, poly(A)-binding protein (PABP), interacting with the 3′end of the viral RNA is partially processed. Proteolysis of eIF3a, eIF3b, PABP and PTB correlated with the extent of cytopathic effect induced by FMDV in infected cells. © 2007 Elsevier Inc. All rights reserved. 2023-02-20T10:38:50Z 2023-02-20T10:38:50Z 2007 journal article Virology 364(2): 466-474 (2007) 0042-6822 http://hdl.handle.net/20.500.12792/3812 http://hdl.handle.net/10261/294448 10.1016/j.virol.2007.03.013 en open Elsevier
institution INIA ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-inia-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del INIA España
language English
topic FMDV
RNA-binding proteins
IRES
S region
3′end
eIF3
PTB
PABP
Proteolytic cleavage
Infected cells
FMDV
RNA-binding proteins
IRES
S region
3′end
eIF3
PTB
PABP
Proteolytic cleavage
Infected cells
spellingShingle FMDV
RNA-binding proteins
IRES
S region
3′end
eIF3
PTB
PABP
Proteolytic cleavage
Infected cells
FMDV
RNA-binding proteins
IRES
S region
3′end
eIF3
PTB
PABP
Proteolytic cleavage
Infected cells
Rodríguez Pulido, M.
Serrano, P.
Sáiz, M.
Martínez-Salas, E.
Foot-and-mouth disease virus infection induces proteolytic cleavage of PTB, eIF3a,b, and PABP RNA-binding proteins
description Foot-and-mouth disease virus (FMDV) infection induces major changes in the host cell including the shutoff of cellular protein synthesis. Here, protein extracts from FMDV-infected cells have been used to monitor changes in the profile of RNA-binding factors interacting with regulatory regions of the viral RNA. Relevant differences have been detected in the pattern of interaction with proteins prepared from either infected or uninfected cells with RNA probes encompassing the internal ribosome entry site (IRES), the 5′ and 3′end regions. The binding patterns obtained for two divergent FMDV isolates showed differences depending on the viral isolate used. The identity of the host proteins giving a shifted binding pattern to RNA regulatory regions has been inferred by immunoblotting. Our results show that polypyrimidine tract-binding protein (PTB) and two subunits of translation initiation factor eIF3 interacting with the IRES undergo proteolytic processing during FMDV infection. In addition, poly(A)-binding protein (PABP), interacting with the 3′end of the viral RNA is partially processed. Proteolysis of eIF3a, eIF3b, PABP and PTB correlated with the extent of cytopathic effect induced by FMDV in infected cells. © 2007 Elsevier Inc. All rights reserved.
format journal article
topic_facet FMDV
RNA-binding proteins
IRES
S region
3′end
eIF3
PTB
PABP
Proteolytic cleavage
Infected cells
author Rodríguez Pulido, M.
Serrano, P.
Sáiz, M.
Martínez-Salas, E.
author_facet Rodríguez Pulido, M.
Serrano, P.
Sáiz, M.
Martínez-Salas, E.
author_sort Rodríguez Pulido, M.
title Foot-and-mouth disease virus infection induces proteolytic cleavage of PTB, eIF3a,b, and PABP RNA-binding proteins
title_short Foot-and-mouth disease virus infection induces proteolytic cleavage of PTB, eIF3a,b, and PABP RNA-binding proteins
title_full Foot-and-mouth disease virus infection induces proteolytic cleavage of PTB, eIF3a,b, and PABP RNA-binding proteins
title_fullStr Foot-and-mouth disease virus infection induces proteolytic cleavage of PTB, eIF3a,b, and PABP RNA-binding proteins
title_full_unstemmed Foot-and-mouth disease virus infection induces proteolytic cleavage of PTB, eIF3a,b, and PABP RNA-binding proteins
title_sort foot-and-mouth disease virus infection induces proteolytic cleavage of ptb, eif3a,b, and pabp rna-binding proteins
publisher Elsevier
publishDate 2007
url http://hdl.handle.net/20.500.12792/3812
http://hdl.handle.net/10261/294448
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