Foot-and-mouth disease virus infection induces proteolytic cleavage of PTB, eIF3a,b, and PABP RNA-binding proteins

Foot-and-mouth disease virus (FMDV) infection induces major changes in the host cell including the shutoff of cellular protein synthesis. Here, protein extracts from FMDV-infected cells have been used to monitor changes in the profile of RNA-binding factors interacting with regulatory regions of the viral RNA. Relevant differences have been detected in the pattern of interaction with proteins prepared from either infected or uninfected cells with RNA probes encompassing the internal ribosome entry site (IRES), the 5′ and 3′end regions. The binding patterns obtained for two divergent FMDV isolates showed differences depending on the viral isolate used. The identity of the host proteins giving a shifted binding pattern to RNA regulatory regions has been inferred by immunoblotting. Our results show that polypyrimidine tract-binding protein (PTB) and two subunits of translation initiation factor eIF3 interacting with the IRES undergo proteolytic processing during FMDV infection. In addition, poly(A)-binding protein (PABP), interacting with the 3′end of the viral RNA is partially processed. Proteolysis of eIF3a, eIF3b, PABP and PTB correlated with the extent of cytopathic effect induced by FMDV in infected cells. © 2007 Elsevier Inc. All rights reserved.

Bibliographic Details

Main Authors:	Rodríguez Pulido, M., Serrano, P., Sáiz, M., Martínez-Salas, E.
Format:	journal article biblioteca
Language:	English
Published:	Elsevier 2007
Subjects:	FMDV, RNA-binding proteins, IRES, S region, 3′end, eIF3, PTB, PABP, Proteolytic cleavage, Infected cells,
Online Access:	http://hdl.handle.net/20.500.12792/3812 http://hdl.handle.net/10261/294448
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