Two related low-temperature-inducible genes of Arabidopsis encode proteins showing high homology to 14-3-3 proteins, a family of putative kinase regulators
We have isolated two Rare Cold-Inducible (RCI1 and RCI2) cDNAs by screening a cDNA library prepared from cold-acclimated etiolated seedlings of Arabidopsis thaliana with a subtracted probe. RNA-blot hybridizations revealed that the expression of both RCI1 and RCI2 genes is induced by low temperature independently of the plant organ or the developmental stage considered. However, RCI1 mRNA accumulates faster and at higher levels than the RCI2 one indicating that these genes have differential responsiveness to cold stress. Additionally, when plants are returned to room temperature, RCI1 mRNA decreases faster than RCI2. In contrast to most of the cold-inducible plant genes characterized, the expression of RCI1 and RCI2 is not induced by ABA or water stress. The nucleotide sequences of RCI1 and RCI2 cDNAs predict two acidic polypeptides of 255 and 251 amino acids with molecular weights of 29 and 28 kDa respectively. The alignment of these polypeptides indicates that they have 181 identical amino acids suggesting that the corresponding genes have a common origin. Sequence comparisons reveal no similarities between the RCI proteins and any other cold-regulated plant protein so far described. Instead, they demonstrate that the RCI proteins are highly homologous to a family of proteins, known as 14-3-3 proteins, which are thought to be involved in the regulation of multifunctional protein kinases. © 1994 Kluwer Academic Publishers.
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Format: | journal article biblioteca |
Language: | English |
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Springer
1994
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Subjects: | Arabidopsis, Cold acclimation, Cold-inducible genes, Freezing tolerance, 14-3-3 proteins, Protein kinase regulators, |
Online Access: | http://hdl.handle.net/20.500.12792/5203 http://hdl.handle.net/10261/293523 |
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dig-inia-es-10261-2935232023-02-20T10:29:20Z Two related low-temperature-inducible genes of Arabidopsis encode proteins showing high homology to 14-3-3 proteins, a family of putative kinase regulators Jarillo Quiroga, José Antonio Capel, J. Leyva, A. Martínez-Zapater, J. M. Salinas, J. Arabidopsis Cold acclimation Cold-inducible genes Freezing tolerance 14-3-3 proteins Protein kinase regulators We have isolated two Rare Cold-Inducible (RCI1 and RCI2) cDNAs by screening a cDNA library prepared from cold-acclimated etiolated seedlings of Arabidopsis thaliana with a subtracted probe. RNA-blot hybridizations revealed that the expression of both RCI1 and RCI2 genes is induced by low temperature independently of the plant organ or the developmental stage considered. However, RCI1 mRNA accumulates faster and at higher levels than the RCI2 one indicating that these genes have differential responsiveness to cold stress. Additionally, when plants are returned to room temperature, RCI1 mRNA decreases faster than RCI2. In contrast to most of the cold-inducible plant genes characterized, the expression of RCI1 and RCI2 is not induced by ABA or water stress. The nucleotide sequences of RCI1 and RCI2 cDNAs predict two acidic polypeptides of 255 and 251 amino acids with molecular weights of 29 and 28 kDa respectively. The alignment of these polypeptides indicates that they have 181 identical amino acids suggesting that the corresponding genes have a common origin. Sequence comparisons reveal no similarities between the RCI proteins and any other cold-regulated plant protein so far described. Instead, they demonstrate that the RCI proteins are highly homologous to a family of proteins, known as 14-3-3 proteins, which are thought to be involved in the regulation of multifunctional protein kinases. © 1994 Kluwer Academic Publishers. 2023-02-20T10:29:20Z 2023-02-20T10:29:20Z 1994 journal article Plant Molecular Biology 25: 693-704 (1994) 0167-4412 http://hdl.handle.net/20.500.12792/5203 http://hdl.handle.net/10261/293523 10.1007/BF00029607 1573-5028 en none Springer |
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Arabidopsis Cold acclimation Cold-inducible genes Freezing tolerance 14-3-3 proteins Protein kinase regulators Arabidopsis Cold acclimation Cold-inducible genes Freezing tolerance 14-3-3 proteins Protein kinase regulators |
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Arabidopsis Cold acclimation Cold-inducible genes Freezing tolerance 14-3-3 proteins Protein kinase regulators Arabidopsis Cold acclimation Cold-inducible genes Freezing tolerance 14-3-3 proteins Protein kinase regulators Jarillo Quiroga, José Antonio Capel, J. Leyva, A. Martínez-Zapater, J. M. Salinas, J. Two related low-temperature-inducible genes of Arabidopsis encode proteins showing high homology to 14-3-3 proteins, a family of putative kinase regulators |
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We have isolated two Rare Cold-Inducible (RCI1 and RCI2) cDNAs by screening a cDNA library prepared from cold-acclimated etiolated seedlings of Arabidopsis thaliana with a subtracted probe. RNA-blot hybridizations revealed that the expression of both RCI1 and RCI2 genes is induced by low temperature independently of the plant organ or the developmental stage considered. However, RCI1 mRNA accumulates faster and at higher levels than the RCI2 one indicating that these genes have differential responsiveness to cold stress. Additionally, when plants are returned to room temperature, RCI1 mRNA decreases faster than RCI2. In contrast to most of the cold-inducible plant genes characterized, the expression of RCI1 and RCI2 is not induced by ABA or water stress. The nucleotide sequences of RCI1 and RCI2 cDNAs predict two acidic polypeptides of 255 and 251 amino acids with molecular weights of 29 and 28 kDa respectively. The alignment of these polypeptides indicates that they have 181 identical amino acids suggesting that the corresponding genes have a common origin. Sequence comparisons reveal no similarities between the RCI proteins and any other cold-regulated plant protein so far described. Instead, they demonstrate that the RCI proteins are highly homologous to a family of proteins, known as 14-3-3 proteins, which are thought to be involved in the regulation of multifunctional protein kinases. © 1994 Kluwer Academic Publishers. |
format |
journal article |
topic_facet |
Arabidopsis Cold acclimation Cold-inducible genes Freezing tolerance 14-3-3 proteins Protein kinase regulators |
author |
Jarillo Quiroga, José Antonio Capel, J. Leyva, A. Martínez-Zapater, J. M. Salinas, J. |
author_facet |
Jarillo Quiroga, José Antonio Capel, J. Leyva, A. Martínez-Zapater, J. M. Salinas, J. |
author_sort |
Jarillo Quiroga, José Antonio |
title |
Two related low-temperature-inducible genes of Arabidopsis encode proteins showing high homology to 14-3-3 proteins, a family of putative kinase regulators |
title_short |
Two related low-temperature-inducible genes of Arabidopsis encode proteins showing high homology to 14-3-3 proteins, a family of putative kinase regulators |
title_full |
Two related low-temperature-inducible genes of Arabidopsis encode proteins showing high homology to 14-3-3 proteins, a family of putative kinase regulators |
title_fullStr |
Two related low-temperature-inducible genes of Arabidopsis encode proteins showing high homology to 14-3-3 proteins, a family of putative kinase regulators |
title_full_unstemmed |
Two related low-temperature-inducible genes of Arabidopsis encode proteins showing high homology to 14-3-3 proteins, a family of putative kinase regulators |
title_sort |
two related low-temperature-inducible genes of arabidopsis encode proteins showing high homology to 14-3-3 proteins, a family of putative kinase regulators |
publisher |
Springer |
publishDate |
1994 |
url |
http://hdl.handle.net/20.500.12792/5203 http://hdl.handle.net/10261/293523 |
work_keys_str_mv |
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_version_ |
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